Corn Straw Residue: a Strategy for Lipase Immobilization

  • Renata Deda Mendonca FerreiraEmail author
  • Rodrigo Brackmann
  • Ernandes Benedito Pereira
  • Raquel Dalla Costa da Rocha


This work aims to study the immobilization of Candida rugosa lipase (CRL) onto corn straw residue. For this purpose, chemical, morphological, and textural characteristics of the corn straw; immobilization process by adsorption; and immobilized enzyme activity and storage stability were evaluated. The corn straw presented isoelectric point of 7.0, surface with hydroxyl bands being favorable to the immobilization process. An irregular surface was also observed with fibers and pores, which are mesoporous and macroporous, characteristics that demonstrate efficiency in mass transfer mechanisms. Upon immobilization, it was observed that adsorption velocity is proportional to the square of the available adsorption sites (pseudo-second-order), and that the immobilization occurs in monolayers (Langmuir isotherm). The adsorption process was favorable and considered as a chemical adsorption mechanism. After immobilization, the optimum temperature increased, the optimum pH reduced, and the affinity of the biocatalyst for the substrate decreased. Corn straw derivative demonstrated good thermal stability. Regarding storage stability, there was approximately 12% loss of activity after 60 days of storage at 4 °C. Considering that no treatment was applied to the corn straw, this result is satisfactory and shows good affinity between this support and CRL.


Candida rugosa Enzyme Adsorption Stability Activity 



The authors are grateful to UFPR-Palotina for the surface and pore size analyses.

Funding Information

This paper was financially supported by CAPES.

Compliance with Ethical Standards

There is no research involving human participants and/or animals.

Conflict of Interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Chemical DepartmentFederal Technological University of Paraná, campus Pato BrancoPato BrancoBrazil
  2. 2.College of Pharmaceutical ScienceFederal University of AlfenasAlfenasBrazil

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