Use of Feruloyl Esterase as Laccase-Mediator System in Paper Bleaching

  • Aysegul Ozer
  • Fulya Ay Sal
  • Ali Osman Belduz
  • Hüseyin Kirci
  • Sabriye CanakciEmail author


In order to bleach the eucalyptus kraft pulp, two enzyme treatments involving feruloyl esterase and laccase were used in the TCF sequence. Hydroxycinnamic acids, which were released from lignin subunits by the activity of feruloyl esterase, were used as a natural mediator of laccase. The use of sequentially feruloyl esterase and laccase has much higher pulp bleaching effects than the individual enzymes. GthFAE, BmegLac and GthFAE+BmegLac treatments (X) reduced the kappa number of eucalyptus kraft pulps by indicating 9%, 18%, and 30% delignification rates, respectively. Just like in delignification rates, the highest brightness improvement was achieved from the GthFAE+BmegLac combination. The results of the present study indicated that the natural mediators, which are presented in the structure of lignin, could be used as laccase-mediators for pulp bleaching more efficiently and cost-effectively.


Feruloyl esterase Hydroxycinnamic acids Laccase Laccase-mediator system 



We would like to thank Dr. Yu Xia (Jiangnan University, China) for providing pMA0911 vector.

Funding Information

This work was partially supported by The Scientific and Technological Research Council of Turkey (grant number 113Z741).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Aysegul Ozer
    • 1
  • Fulya Ay Sal
    • 1
  • Ali Osman Belduz
    • 1
  • Hüseyin Kirci
    • 2
  • Sabriye Canakci
    • 1
    Email author
  1. 1.Department of Biology, Faculty of ScienceKaradeniz Technical UniversityTrabzonTurkey
  2. 2.Department of Forest Industrial Engineering, Faculty of ForestryKaradeniz Technical UniversityTrabzonTurkey

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