The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor

  • Orapin Udomsin
  • Gorawit Yusakul
  • Tharita Kitisripanya
  • Thaweesak Juengwatanatrakul
  • Waraporn PutalunEmail author


To address the high demand for Pueraria candollei var. mirifica (PM) used as the active ingredient in health products and its difficulty to cultivate in the field, the growth and production of deoxymiroestrol (DME) and isoflavonoid (ISF) phytoestrogens in PM cell suspensions were studied. In a 125-mL shake flask, the cell suspension produced DME [78.7 ± 8.79–116 ± 18.2 μg/g dry weight (DW)] and ISF (140 ± 6.83–548 ± 18.5 μg/g DW), which are the predominant ISF glycosides. While ISF aglycones accumulated in the PM cell suspension cultured in the airlift bioreactor. The DME content was increased to 976 ± 79.6 μg/g DW when the PM cell suspension was cultured in the 5-L scale bioreactor. The production of DME and ISF was enhanced by elicitors including methyl jasmonate (MJ), yeast extract (YE), and chitosan (CHI). MJ produced the highest induction of DME accumulation, while ISF accumulation was the highest with YE treatment. Analysis of catalase activity implied that the elicitors enhanced ROS production, which resulted in the enhancement of DME and ISF production and accumulation in PM cell suspension cultures. PM cell suspension culture is a promising source of beneficial PM phytoestrogens that exhibit bioactivity that may useful for the treatment of menopausal symptoms.


Pueraria candollei var. mirifica Cell suspension Bioreactor Deoxymiroestrol Isoflavonoid 



The authors thank Dr. Chaiyo Chaichantipyuth at the Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand, for providing authentic DME and KWA.

Funding Information

This research was financially supported by the Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand, and The Thailand Research Fund (IRN 61W0005).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

12010_2019_3094_MOESM1_ESM.docx (77 kb)
ESM 1 (DOCX 77 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Pharmaceutical SciencesKhon Kaen UniversityKhon KaenThailand
  2. 2.Research Group for Faculty of Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB)National Research University-Khon Kaen UniversityKhon KaenThailand
  3. 3.Drug and Cosmetics Excellence CenterWalailak UniversityThasalaThailand
  4. 4.School of PharmacyWalailak UniversityThasalaThailand
  5. 5.Department of Pharmacognosy, Faculty of PharmacyMahidol UniversityBangkokThailand
  6. 6.Faculty of Pharmaceutical SciencesUbon Ratchathani UniversityUbon RatchathaniThailand

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