Effect of Biohythane Production from Distillery Spent Wash with Addition of Landfill Leachate and Sewage Wastewater

  • Vijaya Krishna Saranga
  • P. Kiran Kumar
  • Kavita Verma
  • D. Bhagawan
  • V. HimabinduEmail author
  • M. Lakshmi Narasu


Rapid development of the industrial and domestic sectors has led to the rise of several energy and environmental issues. In accordance with sustainable development and waste minimization issues, biohydrogen production along with biomethane production via two-stage fermentation process using microorganisms from renewable sources has received considerable attention. In the present study, biohythane production with simultaneous wastewater treatment was studied in a two-stage (Biohydrogen and Biomethane) fermentation process under anaerobic conditions. Optimization of high organic content (COD) distillery spent wash effluent (DSPW) with dilution using sewage wastewater was carried out. Addition of leachate as a nutrient source was also studied for effective biohythane production. The experimental results showed that the maximum biohythane production at optimized concentration (substrate concentration of 60 g/L with 30% of leachate as a nutrient source) was 67 mmol/L bio-H2 and with bio-CH4 production of 42 mmol/L.

Graphical Abstract


Biohydrogen Biomethane Biohythane Distillery spent wash Leachate 



We are thankful to the University Grants Commission (UGC) BSR Fellow, Government of India/Bharat Sarkar - Sanctions order No. F.18-1/2011(BSR) for providing facilities and Dr. D Bhagawan would like to thank the UGC for the encouragement to carry out the research work (order No. F./31-1/2017/PDFSS-2017-18- TEL-14164 and Dr. Kavita Verma would like to acknowledge the Department of Biotechnology (DBT-RA Program in Biotechnology and Life Sciences), Government of India/Bharat Sarkar.

Funding Information

This work received financial support from the University Grants Commission (UGC) BSR Fellow, Government of India/Bharat Sarkar – Sanctions, order no. F.18-1/2011(BSR).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Vijaya Krishna Saranga
    • 1
  • P. Kiran Kumar
    • 2
  • Kavita Verma
    • 2
  • D. Bhagawan
    • 2
  • V. Himabindu
    • 2
    Email author
  • M. Lakshmi Narasu
    • 1
  1. 1.Centre for Biotechnology, Institute of Science and TechnologyJawaharlal Nehru Technological University HyderabadHyderabadIndia
  2. 2.Centre for Alternative Energy Options, Institute of Science and TechnologyJawaharlal Nehru Technological University HyderabadHyderabadIndia

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