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Applied Biochemistry and Biotechnology

, Volume 189, Issue 3, pp 1007–1019 | Cite as

Comparative Transcriptome Analysis of Recombinant Industrial Saccharomyces cerevisiae Strains with Different Xylose Utilization Pathways

  • Yun-Cheng Li
  • Cai-Yun Xie
  • Bai-Xue Yang
  • Yue-Qin TangEmail author
  • Bo Wu
  • Zhao-Yong Sun
  • Min Gou
  • Zi-Yuan Xia
Article

Abstract

A heterologous xylose utilization pathway, either xylose reductase–xylitol dehydrogenase (XR–XDH) or xylose isomerase (XI), is usually introduced into Saccharomyces cerevisiae to construct a xylose-fermenting strain for lignocellulosic ethanol production. To investigate the molecular basis underlying the effect of different xylose utilization pathways on the xylose metabolism and ethanol fermentation, transcriptomes of flocculating industrial strains with the same genetic background harboring different xylose utilization pathways were studied. A different source of xylA did not obviously affect the change of the strains transcriptome, but compared with the XR–XDH strain, several key genes in the central carbon pathway were downregulated in the XI strains, suggesting a lower carbon flow to ethanol. The carbon starvation caused by lower xylose metabolism in XI strains further influenced the stress response and cell metabolism of amino acid, nucleobase, and vitamin. Besides, the downregulated genes mostly included those involved in mitotic cell cycle and the cell division–related process. Moreover, the transcriptomes analysis indicated that the after integrate xylA in the δ region, the DNA and chromosome stability and cell wall integrity of the strains were affected to some extent. The aim of this was to provide some reference for constructing efficient xylose-fermenting strains.

Keywords

Bioethanol Saccharomyces cerevisiae Transcriptome Xylose isomerase Xylose reductase–xylitol dehydrogenase 

Notes

Funding information

This work was supported by the National Natural Science Foundation of China (31170093), the Talent Project for Science and Technology Innovation of Sichuan Province (2017RZ0021), and the Open Fund of Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture of China (2018-009).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interests.

Supplementary material

12010_2019_3060_MOESM1_ESM.docx (201 kb)
ESM 1 (DOCX 201 kb)
12010_2019_3060_MOESM2_ESM.xlsx (85 kb)
ESM 2 (XLSX 85 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Pharmacy and Biological EngineeringChengdu UniversityChengduChina
  2. 2.College of Architecture and EnvironmentSichuan UniversityChengduChina
  3. 3.Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, CAASChengduChina

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