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Applied Biochemistry and Biotechnology

, Volume 189, Issue 2, pp 345–358 | Cite as

Characterization of Glucokinase Catalysis from a Pseudo-Dimeric View

  • Hanchi Chen
  • Xiaoqing Cai
  • Anjie Xu
  • Linjiang Zhu
  • Yuele Lu
  • Xiaolong ChenEmail author
  • Shijie LiuEmail author
Article
  • 286 Downloads

Abstract

Glucose phosphorylation by glucokinase exhibits a sigmoidal dependency on substrate concentration regardless of its simple structure. Dimorph mechanism suggested the existence of two enzymatic states with different catalytic properties, which has been shown to be plausible by structural analysis. However, the dimorph mechanism gives rise to a complicated or non-explicit non-closed mathematical form. It is neither feasible to apply the dimorph mechanism in effector characterizations. To improve the area of glucokinase study with stronger theoretical support and less complication in computation, we proposed the investigation of the enzyme from a pseudo-dimeric angle. The proposed mechanism started from the idealization of two monomeric glucokinase as a dimeric complex, which significantly simplified the glucose phosphorylation kinetics, while the differences in enzyme reconfiguration caused by variable substrates and effectors have been successfully characterized. The study presented a simpler and more reliable way in studying the properties of glucokinase and its effectors, providing guidelines of effector developments for hyperglycemia and hypoglycemia treatment.

Keywords

Glucokinase Biocatalysis Multiple substrates Kinetics Effector 

Notes

Funding Information

This study is financially support from the Zhejiang Provincial Natural Science Foundation of China (No. Y19B060022) and National Natural Science Foundation of China (No. 31601390).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institution of Fermentation EngineeringZhejiang University of TechnologyHangzhouChina
  2. 2.Department of Paper and Bioprocess EngineeringState University of New York College of Environmental Science and ForestrySyracuseUSA

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