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Enzyme-Catalyzed Production of FAME by Hydroesterification of Soybean Oil Using the Novel Soluble Lipase NS 40116

  • Daniela V. Rosset
  • João H. C. Wancura
  • Gustavo A. Ugalde
  • J. Vladimir Oliveira
  • Marcus V. TresEmail author
  • Raquel C. Kuhn
  • Sérgio L. Jahn
Article
  • 9 Downloads

Abstract

The performance of lipase NS 40116, a novel and promising soluble enzyme obtained from modified Thermomyces lanuginosus microorganism, was investigated in the production of biodiesel (fatty acid methyl esters—FAME) by hydroesterification. In order to investigate the potential of the biocatalyst in its soluble form, this work reports the effect of water content and enzyme load, as well as the recovery and reuse of the biocatalyst. A FAME yield of 94.30% after 12 h was achieved at 35 °C by combining 0.50 wt% of lipase, 15 wt% of water, and a methanol:oil molar ratio of 4.5:1. The analysis of the time course reaction suggests that the triacylglycerides (TAGs) are hydrolyzed by the enzyme in a first step, generating free fatty acids (FFAs), followed by the esterification of these FFAs into FAME. In relation to the reusability assays, the lipase kept approximately 90% of its catalytic activity after five cycles of reuse. In this context, the findings of this study demonstrate that lipase NS 40116 can efficiently catalyze hydroesterification reactions under mild conditions, arising as a competitive alternative for biodiesel synthesis.

Keywords

FAME NS 40116 lipase Biodiesel production Hydroesterification Lipase reuse 

Abbreviations

AV

Acid value

FTIR

Fourier transform infrared

FAME

Fatty acid methyl esters

FFA

Free fatty acids

KOH

Potassium hydroxide

TAG

Triacylglycerides

Notes

Funding Information

This work received financial support from the National Council of Technological and Scientific Development (CNPq) and the Research Support Foundation of the State of Rio Grande do Sul (FAPERGS) throughout this research, as well the Coordination for the Improvement of Higher Education Personnel (CAPES) for scholarships.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemical EngineeringFederal University of Santa MariaSanta MariaBrazil
  2. 2.Department of Crop ProtectionFederal University of Santa MariaSanta MariaBrazil
  3. 3.Department of Chemical and Food EngineeringFederal University of Santa CatarinaFlorianopolisBrazil
  4. 4.Laboratory of Agroindustrial Processes Engineering (LAPE)Federal University of Santa MariaCachoeira do SulBrazil

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