Long-Term Monokaryotic Cultures of Pleurotus ostreatus var. florida Produce High and Stable Laccase Activity Capable to Degrade ß-Carotene

  • Diana LinkeEmail author
  • Alejandra B. Omarini
  • Meike Takenberg
  • Sebastian Kelle
  • Ralf G. Berger


An extracellular laccase (Lacc10) was discovered in submerged cultures of Pleurotus ostreatus var. florida bleaching ß-carotene effectively without the addition of a mediator (650 mU/L, pH 4). Heterologous expression in P. pastoris confirmed the activity and structural analyses revealed a carotenoid-binding domain, which formed the substrate-binding pocket and is reported here for the first time. In order to increase activity, 106 basidiospore-derived monokaryons and crosses of compatible progenies were generated. These showed high intraspecific variability in growth rate and enzyme formation. Seventy-two homokaryons exhibited a higher activity-to-growth-rate-relation than the parental dikaryon, and one isolate produced a very high activity (1800 mU/L), while most of the dikaryotic hybrids showed lower activity. The analysis of the laccase gene of the monokaryons revealed two sequences differing in three amino acids, but the primary sequences gave no clue for the diversity of activity. The enzyme production in submerged cultures of monokaryons was stable over seven sub-cultivation cycles.


Basidiomycota Dikaryons Monokaryons Hybrids Destaining Carotenoids Carotenoid-binding domain 



We thank the Institute of Technical Chemistry (Leibniz University Hannover) for the measurements of qPCR raw data and Ulrich Krings for contributing the ab initio peptide sequence.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Diana Linke
    • 1
    Email author
  • Alejandra B. Omarini
    • 2
    • 3
  • Meike Takenberg
    • 1
  • Sebastian Kelle
    • 1
  • Ralf G. Berger
    • 1
  1. 1.Institut für LebensmittelchemieLeibniz Universität HannoverHannoverGermany
  2. 2.Downstream Bioprocessing LabJacobs University Bremen gGmbHBremenGermany
  3. 3.INCITAP (CONICET-UNLPam) Consejo Nacional de Investigaciones Científicas y TécnicasUniversidad Nacional de La PampaSanta RosaArgentina

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