Efficient Production of Polymalic Acid by a Novel Isolated Aureobasidium pullulans Using Metabolic Intermediates and Inhibitors
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Polymalic acid (PMA) is a linear anionic polyester composed of l-malic acid monomers, which have potential applications as drug carriers, surgical suture, and biodegradable plastics. In this study, a novel strain of Aureobasidium pullulans var. melanogenum GXZ-6 was isolated and identified according to the morphological observation and deoxyribonucleic acid internal-transcribed spacer sequence analysis, and the product of PMA was characterized by FT-IR, 13C-NMR, and 1H-NMR spectra. The PMA titer of GXZ-6 reached 62.56 ± 1.18 g L−1 with productivity of 0.35 g L−1 h−1 using optimized medium with addition of metabolic intermediates (citrate and malate) and inhibitor (malonate) by batch fermentation in a 10-L fermentor. Besides that the malate for PMA synthesis in GXZ-6 might mainly come from the glyoxylate cycle, based on results, citrate, malate, malonate, and maleate increased while succinate and fumarate inhibited the production of PMA, which was different from that of other A. pullulans. This study provided a potential strain and a simple metabolic control strategy for high-titer production of PMA and shared novel information on the biosynthesis pathway of PMA in A. pullulans.
KeywordsPolymalic acid Aureobasidium pullulans var. melanogenum High-titer Metabolic control Biosynthesis pathway
This work was financially supported by the National Natural Science Foundation of China (21506039, 31760452, 31560448) and the Natural Science Foundation of Guangxi Province (2016GXNSFAA380140, 2015GXNSFBA139052).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 2.Ding, H., Helguera, G., Rodriguez, J. A., Markman, J., Luria-Perez, R., Gangalum, P., Portilla-Arias, J., Inoue, S., Daniels-Wells, T. R., Black, K., Holler, E., Penichet, M. L., & Ljubimova, J. Y. (2013). Polymalic acid nanobioconjugate for simultaneous immunostimulation and inhibition of tumor growth in HER2/neu-positive breast cancer. Journal of Controlled Release, 171(3), 322–329.CrossRefGoogle Scholar
- 11.Cao, W., Qi, B., Zhao, J., Qiao, C., Su, Y., & Wan, Y. (2013). Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing β-poly (malic acid) production by Aureobasidium pullulans ipe-1. Journal of Chemical Technology & Biotechnology, 88(5), 808–817.CrossRefGoogle Scholar
- 25.Gostincar, C., Ohm, R. A., Kogej, T., Sonjak, S., Turk, M., Zajc, J., Zalar, P., Grube, M., Sun, H., Han, J., Sharma, A., Chiniquy, J., Ngan, C. Y., Lipzen, A., Barry, K., Grigoriev, I. V., & Gunde-Cimerman, N. (2014). Genome sequencing of four Aureobasidium pullulans varieties: biotechnological potential, stress tolerance, and description of new species. BMC Genomics, 15(1), 549.CrossRefGoogle Scholar
- 28.Lee, B. S., & Holler, E. (2000). β-Poly(l-malate) production by non-growing microplasmodia of Physarum polycephalum effects of metabolic intermediates and inhibitors. FEMS Microbiology Letters, 193(1), 69–74.Google Scholar