Applied Biochemistry and Biotechnology

, Volume 187, Issue 1, pp 338–351 | Cite as

Autotrophic, Heterotrophic, and Mixotrophic Nitrogen Assimilation for Single-Cell Protein Production by Two Hydrogen-Oxidizing Bacterial Strains

  • Junwei Dou
  • Yanmeng Huang
  • Haiwei Ren
  • Zhizhong Li
  • Qin Cao
  • Xiaofeng Liu
  • Dong Li


To recover a nitrogen resource from high-ammonia-nitrogen wastewater, two amphitrophic hydrogen-oxidizing bacteria (HOB), Paracoccus denitrificans Y5 and P. versutus D6, capable of nitrogen assimilation for single-cell protein (SCP) production were isolated. These two HOB strains could grow autotrophically with H2 as an electron donor, O2 as an electron acceptor, CO2 as a carbon source, and ammonia nitrogen (NH4+-N) as a nitrogen source. The cell molecular formulas of strains Y5 and D6 determined by autotrophic cultivation were C3.33H6.83O2.58N0.77 and C2.87H5.34O3.17N0.57, respectively. The isolated strains could synchronously remove NH4+-N and organic carbon and produce SCP via heterotrophic cultivation. The rates of removal of NH4+-N and soluble chemical oxygen demand reached 35.47 and 49.04%, respectively, for Y5 under mixotrophic cultivation conditions with biogas slurry as a substrate. SCP content of strains Y5 and D6 was 67.34–73.73% based on cell dry weight. Compared with soybean meal, the SCP of Y5 contained a variety of amino acids.


High-ammonia-nitrogen wastewater Hydrogen-oxidizing bacterium Nitrogen assimilation Single-cell protein Mixotrophy Paracoccus 



This study was supported jointly by the National Key R & D Program of China (2018YFD0501405), by the Youth Innovation Promotion Association CAS (2017423), the Key Project for Foreign Cooperation of the International Cooperation Bureau of the Chinese Academy of Sciences (182344KYSB20170009), the Science and Technology Service Network Initiative (STS) of the Chinese Academy of Sciences, the Key Laboratory of Environmental and Applied Microbiology of Chengdu Institute of Biology CAS (KLCAS-2016-10, KLCAS-2017-9), and the Chengdu Science and Technology Huimin Project (2016-HM02-00092-SF).

Compliance with Ethical Standards

Conflicts of Interest

The authors declare that there are no conflicts of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of BiologyChinese Academy of SciencesChengduPeople’s Republic of China
  2. 2.School of Life Science and EngineeringLanzhou University of TechnologyLanzhouPeople’s Republic of China

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