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Applied Biochemistry and Biotechnology

, Volume 187, Issue 1, pp 163–175 | Cite as

Reduction of Fumarate to Succinate Mediated by Fusobacterium varium

  • Nicholas C. McDonald
  • Robert L. WhiteEmail author
Article
  • 115 Downloads

Abstract

Accumulation of succinate as a fermentation product of Fusobacterium varium was enhanced when the anaerobic bacterium was grown on complex peptone medium supplemented with fumarate. Residual substrates and fermentation products were determined by proton NMR spectroscopy. Cells collected from the fumarate-supplemented medium (8–10 h after inoculation) supported the conversion of fumarate to succinate when suspended with fumarate and a co-substrate (glucose, sorbitol, or glycerol). Succinate production was limited by the availability of fumarate or reducing equivalents supplied by catabolism of a co-substrate via the Embden-Meyerhof-Parnas (EMP) pathway. The choice of reducing co-substrate influenced the yield of acetate and lactate as side products. High conversions of fumarate to succinate were achieved over pH 6.6–8.2 and initial fumarate concentrations up to 300 mM. However, at high substrate concentrations, intracellular retention of succinate reduced extracellular yields. Overall, the efficient utilization of fumarate (≤ 400 mM) combined with the significant extracellular accumulation of succinate (corresponding to ≥ 70% conversion) indicated the effective utilization of fumarate as a terminal electron acceptor by F. varium and the potential of the methodology for the bioproduction of succinate.

Keywords

Biotransformation Bioconversion Fumaric acid reduction Succinic acid Fusobacterium varium Anaerobic metabolism Fermentation Cell suspensions Sorbitol Glycerol 

Notes

Acknowledgements

This research was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC grant RGPIN/04536-2014). NMR and mass spectra were collected on instruments provided by NMR-3 and the Mass Spectrometry Laboratory, respectively (Dalhousie University).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.

Supplementary material

12010_2018_2817_MOESM1_ESM.docx (114 kb)
ESM 1 (DOCX 114 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of ChemistryDalhousie UniversityHalifaxCanada

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