Applied Biochemistry and Biotechnology

, Volume 187, Issue 1, pp 140–151 | Cite as

Influence of Added Nutrients and Substrate Concentration in Biohydrogen Production from Winery Wastewaters Coupled to Methane Production

  • Julián Carrillo-Reyes
  • Blanca Aidé Albarrán-Contreras
  • Germán BuitrónEmail author


Winery wastewaters are acidic effluents with high content of organic matter and nutrients. Different initial values of chemical oxygen demand (COD), ranging from 4 to 50 g L−1, were tested in batch assays to evaluate the fermentative hydrogen production followed by a methane production step. The influence of adding a typical nutrient solution for hydrogen production was investigated. Nutrients include N–NH4, Mg, Fe, Co, Mn, I, Ni, and Zn. The best hydrogen production potential was obtained at a COD of 50 g L−1 without nutrient addition. This condition produced 528 mL H2 L−1. At a COD ≥ 35 g L−1, tests with only WW had a hydrogen potential 1.6 to 1.9 times higher than did tests where nutrients were added. The use of added nutrients reduced the hydrogen production by producing additional reduced acids, such as propionate and valerate. In a second stage, biomethane potential was evaluated using the effluent of a selected condition from hydrogen production tests. The methane production reached values of 207 ± 2.2 mL CH4 g−1 COD at 10 g COD L−1. The COD affected the specific methane production. The results of this study demonstrated the potential of winery effluents as a substrate for sequential hydrogen and methane production to increase the energy recovery from this effluent, with a maximum energetic yield and productivity of 7.15 kJ gCOD−1 and 11.51 kJ d−1.


Biohydrogen Biogas Winery wastewater Dark fermentation Methane Caproic acid 



The authors gratefully acknowledge the financial support of the projects Fondo de Sustentabilidad Energética SENER-CONACYT, Clúster Biocombustibles Gaseosos, 247006 and CONACYT 255537. The technical assistance of Karen Daniela Ramírez Martínez, Gloria Moreno, Jaime Pérez, and Ángel A. Hernández is also acknowledged.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica JuriquillaUniversidad Nacional Autónoma de MéxicoQueretaroMexico

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