Applied Biochemistry and Biotechnology

, Volume 187, Issue 1, pp 129–139 | Cite as

Phycocyanin Production by Aphanothece microscopica Nägeli in Synthetic Medium Supplemented with Sugarcane Vinasse

  • Dayane V. MoraisEmail author
  • Reinaldo G. Bastos


This study focused on the evaluation of mixotrophic and heterotrophic production of phycocyanin by A. microscopica, analysis of kinetic parameters, the effect of freezing and thawing on phycocyanin yield, and nutrient removal during heterotrophic growth. During mixotrophic growth, maximum phycocyanin yield (1.50 mgphycocyanin g−1biomass) was obtained after 12 h, while the heterotrophic cultivation yielded 1.39 mgphycocyanin g−1biomass. The mixotrophic cultivation of A. microscopica showed maximum specific growth rate of 0.025 h−1, against 0.010 h−1 for the photoautotrophic cultivation, and 0.08 h−1 in heterotrophic conditions. The mixotrophic cultivation had a specific rate of phycocyanin production of 9.86 mgphycocyanin mgbiomass−1 h−1, while the photoautotrophic had 2.81 mgphycocyanin mgbiomass−1 h−1, and the heterotrophic, 49.18 mgphycocyanin mgbiomass−1 h−1. Carbon and nitrogen contents present in sugarcane vinasse were decreased in 16.69 and 15.97%, respectively, after 6 h of heterotrophic growth. Thus, it was shown that the mixotrophic production of phycocyanin by Aphanothece microscopica Nägeli in BG11 medium supplemented with vinasse is feasible.


Phycobiliproteins Wastewater Microalgae Cyanobacteria Mixotrophic growth 



The authors would like to thank the Centre for Improvement of Higher Education Personnel (CAPES) for financial support and LAST/CCA/UFSCar for the sugarcane chemical analysis.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Center of Agricultural Sciences (CCA)Federal University of São CarlosArarasBrazil

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