Applied Biochemistry and Biotechnology

, Volume 187, Issue 1, pp 90–100 | Cite as

Predicting Promiscuous T Cell Epitopes for Designing a Vaccine Against Streptococcus pyogenes

  • Samira Ebrahimi
  • Hassan Mohabatkar
  • Mandana Behbahani


One of the most dangerous human pathogens with high prevalence worldwide is Streptococcus pyogenes, which has major impacts on global morbidity and mortality. A major challenge for S. pyogenes vaccine development is the detection of epitopes that confer protection from infection by multiple S. pyogenes types. Our aim was to identify the most conserved and immunogenic antigens of S. pyogenes, which can be a potential candidate for vaccine design in the future. Eight important surface proteins were analyzed. Using different prediction servers, strongest epitopes were selected. They had the ability to stimulate the humoral and cell-mediated immune system. Molecular docking was performed for measuring free-binding energy of selected epitopes. Seven epitopes from three surface proteins were selected as potential candidates for vaccine development. Conservation of selected epitopes among different Streptococcus types was checked. Further in vitro and in vivo tests are required to validate the suitability of the epitopes for vaccine design.


Streptococcus pyogenes T cell epitope prediction Molecular docking Surface protein Vaccine design 


Funding information

This study was supported by the University of Isfahan.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Samira Ebrahimi
    • 1
  • Hassan Mohabatkar
    • 1
  • Mandana Behbahani
    • 1
  1. 1.Department of Biotechnology, Faculty of Advanced Sciences and TechnologiesUniversity of IsfahanIsfahanIran

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