Applied Biochemistry and Biotechnology

, Volume 187, Issue 1, pp 253–265 | Cite as

Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation

  • Jinru Jia
  • Huayou ChenEmail author
  • Bangguo Wu
  • Fengjie Cui
  • Hua Fang
  • Hongcheng Wang
  • Zhong Ni


Multi-strain mixed fermentation can provide a relatively complete lignocellulosic enzyme system compared with single-strain fermentation. This study was firstly to screen strains which have a strong ability to hydrolyse rice straw (RS) enzymatically and enrich true protein (TP). Then, the conditions in the process of SSF, including the optimum inoculum size of mixed strains, inoculation ratio, and different inoculation time of N. crassa 14–8, were optimized. The experimental results showed that the highest TP content could be obtained by using N. crassa 14–8, C. utilis, and P. chrysosporium as mixed strains, and 5 mM Mn2+ and 50 mM veratryl alcohol were used as inducers of lignin peroxidase (LiP) to improve the efficiency of enzymatic hydrolysis. When N. crassa 14–8 was inoculated 1 day later than P. chrysosporium, the total inoculum size was 10%, and the optimum ratio of N. crassa 14–8 to P. chrysosporium was 1:2, the maximum TP yield (8.89%) was obtained, with 123.37% of its increase rate. This work proposed a technique with potential application in large-scale feedstuff protein conversion.


Neurospora crassa Multi-strain fermentation Solid-state fermentation True protein Rice straw 



This work was supported by the Key R&D Program of Jiangsu Province (Modern Agriculture), China (BE2017355); the Agricultural Sci-Tech Self-Innovation Program of Jiangsu Province, CX(17)3044, China; the Open Funding Project of National Key Laboratory of Biochemical Engineering; and Jiangsu Special Research and Development Grant for Northern Jiangsu Area, China (SZ-YC2017001).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jinru Jia
    • 1
    • 2
  • Huayou Chen
    • 1
    • 3
    Email author
  • Bangguo Wu
    • 1
  • Fengjie Cui
    • 1
    • 4
  • Hua Fang
    • 4
  • Hongcheng Wang
    • 1
  • Zhong Ni
    • 1
  1. 1.Institute of Life SciencesJiangsu UniversityZhenjiangP. R. China
  2. 2.Translational Research InstituteHenan Provincial People’s HospitalZhengzhouP. R. China
  3. 3.National Key Laboratory of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingP. R. China
  4. 4.Jiangsu Yancheng Yuanyao Biotechnology Co., Ltd.YanchengP. R. China

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