Applied Biochemistry and Biotechnology

, Volume 186, Issue 3, pp 662–680 | Cite as

Detoxification of Organosolv-Pretreated Pine Prehydrolysates with Anion Resin and Cysteine for Butanol Fermentation

  • Jing Li
  • Suan Shi
  • Maobing TuEmail author
  • Brain Via
  • Fubao Fuelbio Sun
  • Sushil Adhikari


Bioconversion of lignocellulose to biofuels suffers from the degradation compounds formed during pretreatment and acid hydrolysis. In order to achieve an efficient biomass to biofuel conversion, detoxification is often required before enzymatic hydrolysis and microbial fermentation. Prehydrolysates from ethanol organosolv-pretreated pine wood were used as substrates in butanol fermentation in this study. Six detoxification approaches were studied and compared, including overliming, anion exchange resin, nonionic resin, laccase, activated carbon, and cysteine. It was observed that detoxification by anion exchange resin was the most effective method. The final butanol yield after anion exchange resin treatment was comparable to the control group, but the fermentation was delayed for 72 h. The addition of Ca(OH)2 was found to alleviate this delay and improve the fermentation efficiency. The combination of Ca(OH)2 and anion exchange resin resulted in completion of fermentation within 72 h and acetone–butanol–ethanol (ABE) production of 11.11 g/L, corresponding to a yield of 0.21 g/g sugar. The cysteine detoxification also resulted in good detoxification performance, but promoted fermentation towards acid production (8.90 g/L). The effect of salt on ABE fermentation was assessed and the possible role of Ca(OH)2 was to remove the salts in the prehydrolysates by precipitation.


Detoxification Fermentation Butanol Organosolv pretreatment 


Funding Information

This study received financial support from the National Science Foundation (NSF-CBET 1555633), Southeastern Sun Grant Center, United States Department of Agriculture (USDA-2010-38502-21854), and the United States Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) through the Integrated Biomass Supply Systems (IBSS) project (2011-68005-30410).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biosystems EngineeringAuburn UniversityAuburnUSA
  2. 2.Department of Biomedical, Chemical and Environmental EngineeringUniversity of CincinnatiCincinnatiUSA
  3. 3.Center for Bioenergy and BioproductsAuburn UniversityAuburnUSA
  4. 4.Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina

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