Isolation and Evaluation of New Antagonist Bacillus Strains for the Control of Pathogenic and Mycotoxigenic Fungi of Fig Orchards
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Bacillus is an antagonistic bacteria that shows high effectiveness against different phytopathogenic fungi and produces various lytic enzymes, such as chitosanase, chitinase, protease, and gluconase. The aim of this study is to determine Bacillus spp. for lytic enzyme production and to evaluate the antifungal effects of the selected strains for biocontrol of mycotoxigenic and phytopathogenic fungi. A total of 92 endospore-forming bacterial isolates from the 24 fig orchard soil samples were screened for chitosanase production, and six best chitosanolytic isolates were selected to determine chitinase, protease, and N-acetyl-β-hexosaminidase activity and molecularly identified. The antagonistic activities of six Bacillus strains against Aspergillus niger EGE-K-213, Aspergillus foetidus EGE-K-211, Aspergillus ochraceus EGE-K-217, and Fusarium solani KCTC 6328 were evaluated. Fungal spore germination inhibition and biomass inhibition activities were also measured against A. niger EGE-K-213. The results demonstrated that Bacillus mojavensis EGE-B-5.2i and Bacillus thuringiensis EGE-B-14.1i were more efficient antifungal agents against A. niger EGE-K-213. B. mojavensis EGE-B-5.2i has shown maximum inhibition of the biomass (30.4%), and B. thuringiensis EGE-B-14.1i has shown maximum inhibition of spore germination (33.1%) at 12 h. This is the first study reporting the potential of antagonist Bacillus strains as biocontrol agents against mycotoxigenic fungi of fig orchads.
KeywordsLytic enzymes Bacillus Biocontrol Mycotoxigenic fungi
This work was supported by the Research Project Fund of TUBITAK (104O339).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 3.Eltem, R., Taskin, E., & Pazarbasi, S. (2009). Biodiversity and flora of microfungi from sultana-type vineyard soil in Turkey. Fresenius Environmental Bulletin, 18, 82–86.Google Scholar
- 12.Heperkan, D., Moretti, A., Dikmen, C. D. and Logrieco, A. F. (2012). Toxigenic fungi and mycotoxin associated with figs in the Mediterranean area. Phytopathologia Mediterranea, 119–130.Google Scholar
- 15.Salako, A., Sholeye, O., & Dairo, O. (2011). Beyond pest control: a closer look at the health implication of pesticides usage. Journal of Toxicology and Environmental Health Sciences, 4, 37–42.Google Scholar
- 19.Choi, Y. J., Kim, E. J., Piao, Z., Yun, Y. C., & Shin, Y. C. (2004). Purification and characterization of chitosanase from Bacillus sp. strain KCTC 0377BP and its application for the production of chitosan oligosaccharides. Applied and Environmental Microbiology, 70(8), 4522–4531.CrossRefGoogle Scholar
- 20.Taskin, E., Eltem, R., da Silva, E. S., & de Souza, J. V. B. (2008). Screening of Aspergillus strains isolated from vineyards for pectinase production. Journal of Food Agriculture and Environment, 6, 5–7.Google Scholar
- 24.Claus, D. (1986). Genus Bacillus Cohn 1872, 174. Bergey's manual of systematic bacteriology, 2, 1105–1139.Google Scholar
- 26.Sri, R., Tanuwidjaja, F., Rukayadi, Y., Suwanto, A., Suhartono, M. T., Hwang, J. K., & Pyun, Y. R. (2004). Study of thermostable chitinase enzymes from Indonesian Bacillus K29-14. Journal of Microbiology and Biotechnology, 14, 647–652.Google Scholar
- 33.Chun, D.-S., Kang, D.-K., & Kim, H.-K. (2002). Isolation and enzyme production of a neutral protease-producing strain, Bacillus sp. DS-1. Korean Journal of Microbiology and Biotechnology, 30, 346–351.Google Scholar
- 41.Kamil, Z., Saleh, M., & Moustafa, S. (2007). Isolation and identification of rhizosphere soil chitinolytic bacteria and their potential in antifungal biocontrol. Global Journal of Molecular Science, 2, 57–66.Google Scholar
- 43.Sakai, K., Narihara, M., Kasama, Y., Wakayama, M., & Moriguchi, M. (1994). Purification and characterization of thermostable beta-N-acetylhexosaminidase of Bacillus stearothermophilus CH-4 isolated from chitin-containing compost. Applied and Environmental Microbiology, 60(8), 2911–2915.PubMedPubMedCentralGoogle Scholar
- 44.Aslim, B., Sağlam, N., & Beyatli, Y. (2002). Determination of some properties of Bacillus isolated from soil. Turkish Journal of Biology, 26, 41–48.Google Scholar
- 53.Snook, M. E., Mitchell, T., Hinton, D. M., & Bacon, C. W. (2009). Isolation and characterization of Leu7-surfactin from the endophytic bacterium Bacillus mojavensis RRC 101, a biocontrol agent for Fusarium verticillioides. Journal of Agricultural and Food Chemistry, 57(10), 4287–4292.CrossRefGoogle Scholar