Transesterification of Waste Frying Oil and Soybean Oil by Combi-lipases Under Ultrasound-Assisted Reactions
- 263 Downloads
This work describes the use of an ultrasound system for the enzymatic transesterification of oils using combi-lipases as biocatalyst. The reactions were carried out evaluating the individual use of waste oil and fresh soybean oil, and the immobilized lipases CALB, TLL, and RML were used as biocatalysts. It was performed in a mixture design of three factors to obtain the ideal mixture of lipases according to the composition of fatty acids present in each oil, and the main reaction variables were optimized. After 18 h of reaction, ultrasound provided a biodiesel yield of about 90% when using soybean oil and 70% using the waste oil. The results showed that ultrasound technology, in combination with the application of enzyme mixtures, known as combi-lipases, and the use of waste oil, could be a promising route to reduce the overall process costs of enzymatic production of biodiesel.
KeywordsTransesterification reaction Biodiesel Waste oil Soybean oil Combi-lipase Ultrasound system
The authors would like to thank Mr. Ramiro Martinez (Novozymes, Spain S.A.) for kindly supplying the enzymes used in this research.
This work was supported by grants from the Brazilian Coordenação de Aperfoiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflicts of interest.
- 8.Alves, J. S., Vieira, N. S., Cunha, A. S., Silva, A. M., Zachia Ayub, M. A., Fernandez-Lafuente, R., & Rodrigues, R. C. (2014). Combi-lipase for heterogeneous substrates: a new approach for hydrolysis of soybean oil using mixtures of biocatalysts. RSC Advances, 4(14), 6863–6868.CrossRefGoogle Scholar
- 13.Trentin, C. M., Popiolki, A. S., Batistella, L., Rosa, C. D., Treichel, H., de Oliveira, D., & Oliveira, J. V. (2015). Enzyme-catalyzed production of biodiesel by ultrasound-assisted ethanolysis of soybean oil in solvent-free system. Bioprocess and Biosystems Engineering, 38(3), 437–448.CrossRefGoogle Scholar
- 19.Martins, A. B., Schein, M. F., Friedrich, J. L. R., Fernandez-Lafuente, R., Ayub, M. A. Z., & Rodrigues, R. C. (2013). Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: enhanced activity and operational stability. Ultrasonics Sonochemistry, 20(5), 1155–1160.CrossRefGoogle Scholar
- 20.AOCS (1998). Official methods and recommended practices of the American Oil Chemists Society 1–2, Champaign.Google Scholar
- 21.EN, 14103 (2001). Fat and oil derivatives - fatty acid methyl esters (FAME) - determination of esters and linolenic acid methyl esters content. European Committee for Standardization.Google Scholar
- 32.Deba, A. A., Tijani, H. I., Galadima, A. I., Mienda, B. S., Deba, F., & Zargoun, L. M. (2014). Waste cooking oil: a resourceful waste for lipase catalysed biodiesel production. International Journal of Scientific and Research Publications, 4, 1–12.Google Scholar
- 38.Batistella, L., Lerin, L. A., Brugnerotto, P., Danielli, A. J., Trentin, C. M., Popiolski, A., Treichel, H., Oliveira, J. V., & de Oliveira, D. (2012). Ultrasound-assisted lipase-catalyzed transesterification of soybean oil in organic solvent system. Ultrasonics Sonochemistry, 19(3), 452–458.CrossRefGoogle Scholar