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Applied Biochemistry and Biotechnology

, Volume 186, Issue 3, pp 764–778 | Cite as

Tissue Indices of Telomere Length and p53 in Patients with Different Gastrointestinal Tumors: Correlation with Clinicopathological Status

  • Hala M. ElBadre
  • Reham I. El-Mahdy
  • Nahed A. Mohamed
  • Madeha M. Zakhary
  • Doaa W. Maximous
Article
  • 54 Downloads

Abstract

Telomere length dysfunction is involved in the generation of genomic rearrangements that drive progression to malignancy. A set of serological markers for telomere dysfunction, namely chitinase and N-acetylglucosaminidase (NAG), DNA damage, and tissue alteration of p53 have been identified. The probability that genomic damage, accumulation of reactive oxygen species, and shorter telomeres may be related to the onset and advancement of gastrointestinal (GI) tumors. A total of 40 patients with GI tumors and 20 healthy controls with matched age and sex were included. Estimation of serum chitinase, NAG, lipid peroxide (LPER), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase by colorimetric methods, and p53 by ELISA were assessed. Related clinicopathological features were determined. Serological chitinase, NO, LPER, and p53 were significantly increased, SOD was significantly decreased (p ˂ 0.001 for each) in GI tumor patients compared with controls and correlated significantly with age. There was a significant correlation between telomere dysfunction indices, p53, oxidative stress indices, and malignant stages of GI cancer patients. Moreover, a significant difference in the mean serum levels of indices between control, malignant, and benign subjects was found. Accordingly, these biomarkers play an important role in the pathogenesis of GI cancer and their estimation may predict the GI tumor behavior.

Keywords

Chitinase Gastrointestinal tumors NAG Oxidative stress markers p53 Telomere dysfunction 

Notes

Funding

This research was funded by the authors themselves.

Compliance with Ethical Standards

The study was conducted in accordance with the Declaration of Helsinki and approved by the local Clinical Research Ethics Committee.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Hala M. ElBadre
    • 1
  • Reham I. El-Mahdy
    • 1
  • Nahed A. Mohamed
    • 1
  • Madeha M. Zakhary
    • 1
  • Doaa W. Maximous
    • 2
  1. 1.Department of Medical Biochemistry, Faculty of MedicineAssiut UniversityAssiutEgypt
  2. 2.Department of Surgical Oncology, South Egypt Cancer InstituteAssiut UniversityAssiutEgypt

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