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Applied Biochemistry and Biotechnology

, Volume 186, Issue 3, pp 547–562 | Cite as

Novel Naphthalimide Derivatives as Selective G-Quadruplex DNA Binders

  • Ufuk Yildiz
  • Burak Coban
Article
  • 179 Downloads

Abstract

A new derivate of 4-bromo-1,8-naphthalic anhydride and its quaternized analogue have been prepared and characterized. The interactions of both derivatives with human telomere quadruplex-DNA and ds-DNA have been comparatively studied by UV-visible (UV-Vis), fluorescent intercalator displacement assays, competition dialysis, circular dichroism (CD), agarose gel electrophoresis, and polyacrylamide gel electrophoresis. The results show that both derivatives can stabilize G-quadruplexes DNA, and they show different binding affinities for G-quadruplexes-DNA and ds-DNA. All spectroscopic studies have shown that the derivatives have a modest selectivity for G-quadruplex versus ds-DNA.

Keywords

4-Bromo-1,8-naphthalic anhydride Quaternized Naphthalimide DNA binding G-Quadruplex DNA selectivity 

Notes

Acknowledgements

We are grateful for the support of Bulent Ecevit University with grant no. 2014-72118496-04.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.

Supplementary material

12010_2018_2749_MOESM1_ESM.docx (7 mb)
ESM 1 (DOCX 7212 kb)

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Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of Arts and SciencesBulent Ecevit UniversityZonguldakTurkey

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