Effect of Enzymatic Digestion of Protein Derivatives Obtained from Mucuna pruriens L. on Production of Proinflammatory Mediators by BALB/c Mouse Macrophages
- 76 Downloads
Inflammation is considered to be a major risk factor for the pathogenesis of chronic non-communicable diseases. Macrophages are important immune cells, which regulate inflammation and host defense by secretion of proinflammatory mediators. Obtaining biopeptides by enzymatic hydrolysis adds value to proteins of vegetative origin, such as Mucuna pruriens L. The present study evaluated the effect of enzymatic digestion of protein derivatives obtained from M. pruriens L. on the production of proinflammatory mediators by BALB/c mouse macrophages. Five different molecular weight peptide fractions were obtained (F > 10, 5–10, 3–5, 1–3, and < 1 kDa, respectively). At 300 μg/mL, F5–10 kDa inhibited 50.26 and 61.00% NO and H2O2 production, respectively. Moreover, F5–10 kDa reduced the IL-6 and TNFα levels to 60.25 and 69.54%, respectively. After enzymatic digestive simulation, F5–10 kDa decreased the inflammatory mediators.
KeywordsBioactive compounds Anti-inflammatory activity Protein hydrolyzate Inflammation
This paper is part of the “Scientific Research Development Directed to develop Protein Derivative of Mucuna pruriens with Potential Biological Activity for Prevention and / or Treatment of Chronic Diseases Associated with Overweight and Obesity” project funded by CONACYT-Mexico (Project 154307).
Compliance with Ethical Standards
Conflict of Interest
The authors declare they have no conflicts of interest.
- 3.Herrera, F., Ruiz, J., Betancur, D., & Segura, M. (2016). Potential therapeutic applications of Mucuna pruriens peptide fractions purified by high-performance liquid chromatography as angiotensin-converting enzyme inhibitors, antioxidants, antithrombotic and hypocholesterolemic agents. Journal of Medicinal Food, 19(2), 187–195.CrossRefGoogle Scholar
- 4.Hirota, T., Nonaka, A., Matsushita, A., Uchida, N., Ohki, K., Asakura, M., & Kitakaze, M. (2011). Milk casein-derived tripeptides, VPP and IPP induced NO production in cultured endothelial cells and endothelium-dependent relaxation of isolated aortic rings. Heart and Vessels, 26(5), 549–556.CrossRefGoogle Scholar
- 11.Abarikwu, S. O. (2014). Kolaviron, a natural flavonoid from the seeds of Garcinia kola, reduces LPS-induced inflammation in macrophages by combined inhibition of IL-6 secretion, and inflammatory transcription factors, ERK1/2, NF-kappaB, p38, Akt, p-c-JUN and JNK. Biochimica et Biophysica Acta, 1840(7), 2373–2381.CrossRefGoogle Scholar
- 17.Gómez, R., Hernández, H., Tamez, P., Tamez, R., Quintanilla, R., Monreal, E., & Rodríguez, C. (2010). Antitumor and immunomodulating potencial of Coriandrum sativum, Piper nigrum and Cinnamomum zeylanicum. Journal Natural Products, 3, 54–63.Google Scholar
- 18.HANNA Instruments. HI 3844 Test Kit de Peróxido de Hidrógeno.Google Scholar
- 19.ELISA technical guide and protocols TR0065, 0. TECH TIP #65. Thermo scientific. 2010 Thermo Fisher Scientific Inc.Google Scholar
- 21.Cu, T., Betancur, D., Gallegos, S., Sandoval, M., & Chel, L. (2015). Studies in vitro inhibition of the angiotensin-converting enzyme-I, hypotensive and antihypertensive effects of peptide fractions of V. unguiculata. Nutrición Hospitalaria, 32, 2117–2125.Google Scholar
- 23.Wu, R., Wu, C., Liu, D., Yang, X., Huang, J., Zhang, J., Liao, B., He, H. L., & Li, H. (2015). Overview of antioxidant peptides derived from marine resources: the sources, characteristic, purification, and evaluation methods. Applied Biochemistry and Biotechnology, 176(7), 1815–1833. https://doi.org/10.1007/s12010-015-1689-9.CrossRefPubMedGoogle Scholar
- 28.Wink, D. A., Nims, R. W., Darbyshire, J. F., Christodoulou, D., Hanbauer, I., Cox, G. W., Laval, F., Laval, J., Cook, J. A., Krishna, M. C., DeGraff, W. G., & Mitchell, J. B. (1994). Reaction kinetics for nitrosation of cysteine and glutathione in aerobic nitric oxide solutions at neutral pH. Insights into the fate and physiological effects of intermediates generated in the NO/O2 reaction. Chemical Research & Toxicology, 7(4), 519–525.CrossRefGoogle Scholar