Applied Biochemistry and Biotechnology

, Volume 186, Issue 3, pp 597–612 | Cite as

Effect of Enzymatic Digestion of Protein Derivatives Obtained from Mucuna pruriens L. on Production of Proinflammatory Mediators by BALB/c Mouse Macrophages

  • Edwin E. Martínez Leo
  • Victor E. Arana Argáez
  • Juan J. Acevedo Fernández
  • Rosa Moo Puc
  • Maira R. Segura CamposEmail author


Inflammation is considered to be a major risk factor for the pathogenesis of chronic non-communicable diseases. Macrophages are important immune cells, which regulate inflammation and host defense by secretion of proinflammatory mediators. Obtaining biopeptides by enzymatic hydrolysis adds value to proteins of vegetative origin, such as Mucuna pruriens L. The present study evaluated the effect of enzymatic digestion of protein derivatives obtained from M. pruriens L. on the production of proinflammatory mediators by BALB/c mouse macrophages. Five different molecular weight peptide fractions were obtained (F > 10, 5–10, 3–5, 1–3, and < 1 kDa, respectively). At 300 μg/mL, F5–10 kDa inhibited 50.26 and 61.00% NO and H2O2 production, respectively. Moreover, F5–10 kDa reduced the IL-6 and TNFα levels to 60.25 and 69.54%, respectively. After enzymatic digestive simulation, F5–10 kDa decreased the inflammatory mediators.


Bioactive compounds Anti-inflammatory activity Protein hydrolyzate Inflammation 



This paper is part of the “Scientific Research Development Directed to develop Protein Derivative of Mucuna pruriens with Potential Biological Activity for Prevention and / or Treatment of Chronic Diseases Associated with Overweight and Obesity” project funded by CONACYT-Mexico (Project 154307).

Compliance with Ethical Standards

Conflict of Interest

The authors declare they have no conflicts of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Edwin E. Martínez Leo
    • 1
  • Victor E. Arana Argáez
    • 2
  • Juan J. Acevedo Fernández
    • 3
  • Rosa Moo Puc
    • 4
  • Maira R. Segura Campos
    • 1
    Email author
  1. 1.Facultad de Ingeniería QuímicaUniversidad Autónoma de YucatánMéridaMexico
  2. 2.Facultad de QuímicaUniversidad Autónoma de YucatánMéridaMexico
  3. 3.Facultad de MedicinaUniversidad Autónoma del Estado de MorelosCuernavacaMexico
  4. 4.Centro Médico Ignacio García Téllez, Instituto Mexicano del Seguro SocialUnidad de Investigación Médica Yucatán, Unidad Médica de Alta EspecialidadMéridaMexico

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