Enhanced Inulin Saccharification by Self-Produced Inulinase from a Newly Isolated Penicillium sp. and its Application in d-Lactic Acid Production
- 195 Downloads
In order to find an alternative for commercial inulinase, a strain XL01 identified as Penicillium sp. was screened for inulinase production. The broth after cultivated was centrifuged, filtered, and used as crude enzyme for the following saccharification. At pH 5.0 and 50 °C, the crude enzyme released 84.9 g/L fructose and 20.7 g/L glucose from 120 g/L inulin in 72 h. In addition, simultaneous saccharification and fermentation of chicory flour for d-lactic acid production was carried out using the self-produced crude inulinase and Lactobacillus bulgaricus CGMCC 1.6970. A high d-lactic acid titer and productivity of 122.0 g/L and 1.69 g/(L h) was achieved from 120 g/L chicory flour in 72 h. The simplicity for inulinase production and the high efficiency for d-lactic acid fermentation provide a perspective and profitable industrial biotechnology for utilization of the inulin-rich biomass.
KeywordsInulinase Chicory flour Penicillium sp. d-Lactic acid Simultaneous saccharification and fermentation
This study was supported by the National Natural Science Foundation of China (51776099, 31300487), the Key Research and Development Program of Jiangsu Province of China (BF2015007). The authors are also grateful to the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 5.Li, L., Chen, C., Li, K., Wang, Y., Gao, C., Ma, C., & Xu, P. (2014). Efficient simultaneous saccharification and fermentation of inulin to 2,3-butanediol by thermophilic Bacillus licheniformis ATCC 14580. Applied and Environmental Microbiology, 80(20), 6458–6464.CrossRefPubMedPubMedCentralGoogle Scholar
- 7.Shi, N., Mao, W., He, X., Chi, Z., Chi, Z., & Liu, G. (2017). Co-expression of exo-inulinase and endo-inulinase genes in the oleaginous yeast Yarrowia lipolytica for efficient single cell oil production from inulin. Applied Biochemistry and Biotechnology. https://doi.org/10.1007/s12010-017-2659-1.
- 9.Park, J. M., Oh, B. R., Kang, I. Y., Heo, S. Y., Seo, J. W., Park, S. M., Hong, W. K., & Kim, C. H. (2017). Enhancement of 2, 3-butanediol production from Jerusalem artichoke tuber extract by a recombinant Bacillus sp. strain BRC1 with increased inulinase activity. Journal of Industrial Microbiology & Biotechnology, 44(7), 1107–1113.CrossRefGoogle Scholar
- 16.Smit, E., Leeflang, P., Glandorf, B., van Elsas, J. D., & Wernars, K. (1999). Analysis of fungal diversity in the wheat rhizosphere by sequencing of cloned PCR-amplified genes encoding 18S rRNA and temperature gradient gel electrophoresis. Applied and Environmental Microbiology, 65, 2614–2621, 6.Google Scholar
- 19.Singh, R. S., Chauhan, K., Singh, J., Pandey, A., & Larroche, C. (2018). Solid-state fermentation of carrot pomace for the production of inulinase by Penicillium oxalicum BGPUP-4. Food Technology and Biotechnology, 56(1).Google Scholar