Loop-Mediated Isothermal Amplification Using a Lab-on-a-Disc Device with Thin-film Phase Change Material
The design and fabrication of temperature measurement systems that facilitate successful realization of DNA amplification using a lab-on-a-disc (LOD) device are a highly challenging task. The major challenge lies in the fact that such a system must be directly attached to a heating chamber in a way that enables the accurate measurement of temperature of the chamber while allowing the LOD to rotate. This paper presents a temperature control system for implementing isothermal amplification of DNA samples using an LOD device. The proposed system utilizes a thin-film phase change material and non-contact heating system to remotely measure the actual temperature of the chamber and, if required, rapidly heat it to the desired temperature. The results of the experiments performed in this study demonstrate that the proposed system provides an automated platform for molecular amplification and exhibits an operational performance comparable to that of traditional microcentrifuge tube-based isothermal amplification systems.
KeywordsLab-on-a-disc POCT DNA amplification Centrifugal microfluidics Loop-mediated isothermal amplification
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 17.Moghimi, H., Moradi, A., Hamedi, J., & Basiri, M. (2016). Development of a loop-mediated isothermal amplification assay for rapid and specific identification of ACT producing Alternaria alternata, the agent of brown spot disease in tangerine. Applied Biochemistry and Biotechnology, 178(6), 1207–1219.CrossRefPubMedGoogle Scholar
- 20.Phaneuf, C., Light, Y. K., Tran, H., Singh, A. K., Koh, C. Y. (2015). Non-contact heating system for a centrifugal microlfuidic platform (no. SAND2015-8608C), Sandia National Laboratories (SNL-CA), Livermore, CA (United States).Google Scholar
- 26.Yoo, J. C. (2016). Bio drive apparatus, and assay method using the same, Patent No: US 9,279,818 B2 (Mar. 8).Google Scholar
- 27.Kuniyuki, K. (2007). Optical disk apparatus and a sliding driving mechanism for an optical pickup thereof, Patent No: US 7,266,060 B2 (Sep. 4).Google Scholar