Applied Biochemistry and Biotechnology

, Volume 186, Issue 3, pp 590–596 | Cite as

Direct Utilization of Non-pretreated Hydrolytic Liquid of Dried Distiller’s Grains with Solubles for Bio-Ethanol by Rhizopus arrhizus RH 7-13-9#

  • Huan Liu
  • Shiwei Zhang
  • Na Yu
  • Liting Dou
  • Li DengEmail author
  • Fang Wang
  • Tianwei Tan


Bio-ethanol, as an environment friendly and renewable fuel, has gained increasing worldwide attention and can be produced through the fermentation of the carbohydrates or sugar(s) fraction of biomass materials. Here, dried distiller’s grains with solubles (DDGS), a waste in production of bio-ethanol, were applied to prepare acid-hydrolytic liquid and it was directly used as seed culture and fermentation medium without pretreatment. Rhizopus arrhizus RH 7-13-9# cultured in non-pretreated acid-hydrolytic liquid with pH 4.7 for 30 h could utilize the concentrated hydrolytic liquid as well as the hydrolytic liquid mixed with glucose. A high yield of bio-ethanol was obtained. It was proven that common used medium could be replaced by non-pretreated acid-hydrolytic liquid to some extent. The pretreated process of acid-hydrolytic liquid was avoided that decreased feed stock cost and was significant for the utilization of acid-hydrolytic liquid from lignocellulose materials.


Bio-ethanol Dried distiller’s grains with solubles (DDGS) Fermentation Rhizopus arrhizus 



This research was financially supported by National Key research program (2016YFD0400601, 2017YFD0400603, 2017YFB0306900), the Natural Science Foundation of China (21476017), the Hong Kong, Macao, and Taiwan scientific and technological cooperation projects (2015DFT30050), the Amoy Industrial Biotechnology R&D and Pilot Conversion Platform (3502Z20121009), and the Fundamental Research Funds for the Central Universities (PYBZ1712).

Compliance with Ethical Standards

Conflicts of Interest

The authors declared that they have no conflicts of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Huan Liu
    • 1
  • Shiwei Zhang
    • 1
  • Na Yu
    • 1
  • Liting Dou
    • 1
  • Li Deng
    • 1
    • 2
    Email author
  • Fang Wang
    • 1
  • Tianwei Tan
    • 1
  1. 1.Beijing Bioprocess Key Laboratory, State Key Laboratory of Chemical Resource EngineeringBeijing University of Chemical TechnologyBeijingPeople’s Republic of China
  2. 2.Amoy - BUCT Industrial Bio-technovation InstituteAmoyPeople’s Republic of China

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