Effect of High Pressure on Paracoccus denitrificans Growth and Polyhydroxyalkanoates Production from Glycerol
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The performance of fermentation under non-conventional conditions, such as high pressure (HP), is a strategy currently tested for different fermentation processes. In the present work, the purpose was to apply HP (10–50 MPa) to fermentation by Paracoccus denitrificans, a microorganism able to produce polyhydroxyalkanoates (PHA) from glycerol. In general, cell growth and glycerol consumption were both reduced by HP application, more extensively at higher pressure levels, such as 35 or 50 MPa. PHA production and composition was highly dependent on the pressure applied. HP was found to decrease polymer titers, but increase the PHA content in cell dry mass (%), indicating higher ability to accumulate these polymers in the cells. In addition, some levels of HP affected PHA monomeric composition, with the polymer produced at 10 and 35 MPa showing considerable differences relative to the ones obtained at atmospheric pressure. Therefore, it is possible to foresee that the changes in polymer composition may also affect its physical and mechanical properties. Overall, the results of this study demonstrated that HP technology (at specific levels) can be applied to P. denitrificans fermentations without compromising the ability to produce PHA, with potentially interesting effects on polymer composition.
KeywordsFermentation Cell growth Glycerol Stress High pressure
This work was supported by the FCT/MEC (QOPNA research Unit, FCT UID/QUI/00062/2019), through national funds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement. The authors Maria J. Mota and Rita P. Lopes were supported by FCT (Fundação para a Ciência e a Tecnologia), with the grants SFRH/BD/97061/2013 and SFRH/BD/97062/2013, respectively.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 2.Kolesárová, N., Hutnan, M., Bodík, I., & Špalková, V. (2011). Utilization of biodiesel by-products for biogas production. BioMed Research International, 2011, 126798.Google Scholar
- 5.Yamane, T., Chen, X., & Ueda, S. (1996). Growth-associated production of poly(3-hydroxyvalerate) from n-pentanol by a methylotrophic bacterium, Paracoccus denitrificans. Applied and Environmental Microbiology, 62(2), 380–384.Google Scholar
- 6.Ueda, S., Matsumoto, S., Takagi, A., & Yamane, T. (1992). Synthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from methanol and n-amyl alcohol by the methylotrophic bacteria Paracoccus denitrificans and Methylobacterium extorquens. Applied and Environmental Microbiology, 58(11), 3574–3579.Google Scholar
- 10.Mota, M. J., Lopes, R. P., Koubaa, M., Roohinejad, S., Barba, F. J., Delgadillo, I., & Saraiva, J. A. (2018). Fermentation at non-conventional conditions in food-and bio-sciences by the application of advanced processing technologies. Critical Reviews in Biotechnology, 38(1), 122–140.CrossRefGoogle Scholar
- 15.Follonier, S., Henes, B., Panke, S., & Zinn, M. (2012). Putting cells under pressure: a simple and efficient way to enhance the productivity of medium-chain-length polyhydroxyalkanoate in processes with Pseudomonas putida KT2440. Biotechnology and Bioengineering, 109(2), 451–461.CrossRefGoogle Scholar
- 19.Kato, C., & Qureshi, M. H. (1999). Pressure response in deep-sea piezophilic bacteria. Journal of Molecular Microbiology and Biotechnology, 1(1), 87–92.Google Scholar
- 26.Davis, R., Kataria, R., Cerrone, F., Woods, T., Kenny, S., O’Donovan, A., et al. (2013). Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains. Bioresource Technology, 150, 202–209.CrossRefGoogle Scholar
- 29.Üçisik-Akkaya, E., Ercan, O., Yesiladali, S. K., Öztürk, T., Ubay-Çokgör, E., Orhon, D., et al. (2009). Enhanced polyhydroxyalkanoate production by Paracoccus pantotrophus from glucose and mixed substrate. Fresenius Environmental Bulletin, 18(11), 2013–2022.Google Scholar
- 31.Huijberts, G. N., Eggink, G., De Waard, P., Huisman, G. W., & Witholt, B. (1992). Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers. Applied and Environmental Microbiology, 58(2), 536–544.Google Scholar
- 36.Shahid, S., Mosrati, R., Ledauphin, J., Amiel, C., Fontaine, P., Gaillard, J.-L., & Corroler, D. (2013). Impact of carbon source and variable nitrogen conditions on bacterial biosynthesis of polyhydroxyalkanoates: evidence of an atypical metabolism in Bacillus megaterium DSM 509. Journal of Bioscience and Bioengineering, 116(3), 302–308.CrossRefGoogle Scholar
- 37.Ribeiro, P. L. L., da Silva, A. C. M. S., Menezes Filho, J. A., & Druzian, J. I. (2015). Impact of different by-products from the biodiesel industry and bacterial strains on the production, composition, and properties of novel polyhydroxyalkanoates containing achiral building blocks. Industrial Crops and Products, 69, 212–223.CrossRefGoogle Scholar
- 39.Ribeiro, P. L. L., Souza Silva, G., & Druzian, J. I. (2016). Evaluation of the effects of crude glycerol on the production and properties of novel polyhydroxyalkanoate copolymers containing high 11-hydroxyoctadecanoate by Cupriavidus necator IPT 029 and Bacillus megaterium IPT 429. Polymers for Advanced Technologies, 27(4), 542–549.CrossRefGoogle Scholar