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Effect of Different Carbon Sources on Cellulase Production by Marine Strain Microbulbifer hydrolyticus IRE-31-192

  • Huan Liu
  • Liping Zeng
  • Yuhan Jin
  • Kaili Nie
  • Li DengEmail author
  • Fang Wang
Article
  • 23 Downloads

Abstract

Cellulase is an important enzyme that can be used to breakdown lignocellulose into glucose. Microbulbifer hydrolyticus IRE-31(ATCC 700072) is a kind of marine bacterium, which could grow in high salinity medium and has fast-strong growth ability. In this study, a novel strain was screened from Microbulbifer hydrolyticus IRE-31 through mutations to produce cellulase. The effect of different carbon sources on the growth as well as on the production of cellulase of the new strain was studied. Carboxymethyl-cellulase (CMCase) activity selected to represent cellulase was proven to be effectively promoted while xylose, galactose, and melibiose as well as glucose were used as carbon sources. When xylose and glucose were chosen to be further investigated, 472.57 U/L and 266.01 U/L CMCase activity were obtained from 30 g/L glucose and 10 g/L xylose, respectively. These results clarified the effect of different carbon sources on the production of cellulase, which laid a good foundation for the further research in the production of cellulase by marine bacteria.

Keywords

Marine bacteria Cellulase CMCase Fermentation 

Notes

Funding Information

This research was financially supported by the National Key Research Program (2016YFD0400601, 2017YFD0400603, 2017YFB0306900), the Natural Science Foundation of China (21476017), the Hong Kong, Macao, and Taiwan Scientific And Technological Cooperation Projects (2015DFT30050), and the Amoy Industrial Biotechnology R&D and Pilot Conversion Platform (3502Z20121009).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Huan Liu
    • 1
  • Liping Zeng
    • 1
  • Yuhan Jin
    • 1
  • Kaili Nie
    • 1
    • 2
  • Li Deng
    • 1
    • 2
    Email author
  • Fang Wang
    • 1
  1. 1.Beijing Bioprocess Key Laboratory, State Key Laboratory of Chemical Resource EngineeringBeijing University of Chemical TechnologyBeijingPeople’s Republic of China
  2. 2.Amoy - BUCT Industrial Bio-technovation InstituteAmoyPeople’s Republic of China

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