Enhanced Performance of Rhizopus oryzae Lipase by Reasonable Immobilization on Magnetic Nanoparticles and Its Application in Synthesis 1,3-Diacyglycerol

  • Jiong-feng Zhao
  • Jian-ping Lin
  • Li-rong Yang
  • Mian-bin WuEmail author


Nano-sized Fe3O4 was synthesized by chemical co-precipitation and subsequently modified with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde to introduce aldehyde group on its surface. With the help of “interface activation” by adding sucrose esters-11 as surfactant, lipase from Rhizopus oryzae was successfully immobilized onto the carrier with great enhancement of activity. The hydrolysis activity of immobilized enzyme were 9.16 times and 31.6 times of free enzyme when p-nitrophenol butyrate and p-nitrophenol palmitate were used as substrates. The thermo-stability of immobilized enzyme was also enhanced compared to free enzyme. The immobilized enzyme was successfully applied in synthesis of 1,3-diacyglycerols (1,3-DAG). The specific esterification activity of immobilized enzyme was about 1.5 times of the free enzyme. The immobilized enzyme showed good region-selectivity towards 1,3-diacyglycerols and retained nearly 80% of its activity after reused for 60 times, revealing a good industrial application prospect.


Nano-sized Fe3O4 Rhizopus oryzae lipase Interface activated Immobilization 1,3-diacyglycerols 



This work was supported by the National Natural Science Foundation of China [Grant No. 21376215]; National Basic Research Program of China [973, 2011CB710803]; and the National High-Tech Research and Development Program of China [863, 2012AA022302].

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Authors and Affiliations

  1. 1.Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological EngineeringZhejiang UniversityHangzhouChina

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