Nanosilicalites as Support for β-Glucosidases Covalent Immobilization
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Many different materials have been tested for β-glucosidases immobilization. Such materials, however, often show a poor activity related to a low surface area of the support or even enzyme hindrance caused by entrapment inside porous matrix. In this context, the use of nanosized zeolites as enzymes support is quite new and may be an interesting alternative. The present work evaluates the immobilization of β-glucosidases in nanosized silicalites by covalent coupling. The new biocatalyst was able to convert 100% of cellobiose into glucose in 18 h at 50 °C and pH 5, retaining 85% of its activity after five cycles of reuse. A detailed investigation of the published literature indicates that, apparently, this is the first work concerning the immobilization of β-glucosidases on nanosized zeolites ever reported.
KeywordsEnzymes Immobilization Zeolites Cellobiose Silicalite β-glucosidase
The authors are grateful to Thomas Davies for the TEM analysis at the Research Complex at Harwell through the UK catalysis hub funded by EPSRC (portfolio grants EP/K014706/1, EP/K014668/1, EP/K014854/1, and EP/K014714/1). The authors gratefully acknowledge the financial support of CNPq, CAPES-Brazil, EPSRC (grant EP/K014773/1), the Department for Business Skills and Innovation (Regional Growth Fund, MicroBioRefinery project) and the Centre for Materials Discovery.
- 4.Figueira Jde, A., Dias, F. F., Sato, H. H., & Fernandes, P. (2011). Screening of supports for the immobilization of beta-glucosidase. Enzyme Research, 2011, 642460.Google Scholar
- 13.Maeda, R. N., Barcelos, C. A., Santa Anna, L. M., & Pereira Jr., N. (2013). Cellulase production by Penicillium funiculosum and its application in the hydrolysis of sugar cane bagasse for second generation ethanol production by fed batch operation. Journal of Biotechnology, 163, 38–44.CrossRefGoogle Scholar
- 14.Maeda, R. N., Serpa, V. I., Rocha, V. A. L., Mesquita, R. A. A., Anna, L. M. M. S., de Castro, A. M., Driemeier, C. E., Pereira, N., & Polikarpov, I. (2011). Enzymatic hydrolysis of pretreated sugar cane bagasse using Penicillium funiculosum and Trichoderma harzianum cellulases. Process Biochemistry, 46, 1196–1201.CrossRefGoogle Scholar