Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

A Bio-Electrochemical Calculation Model for Color Decline Kinetics of Bruised “Shine Muscat” Fruit During Storage


Physical damage of fruits and vegetables during distribution accelerate their decline in quality during storage. This study was aimed to analyze the quality degradation that takes injuries into consideration by using estimation models. Estimation models for bruised “Shine Muscat” grapes during storage were investigated based on bio-electrochemical theory. An electrical indicator, LTO, defined as the length from the origin of the coordinate at the top of the circular arc of the Cole−Cole plot, was demonstrated to effectively quantify the degree of a bruise with high accuracy (r2 > 99). The color-change kinetics constant increased as LTO decreased, and a strong correlation was confirmed between these two parameters (p < 0.01). These results demonstrate that the LTO parameter can be used to characterize physical damage incurred by the fruit, and it may be used as an injury parameter to calculate their color-change kinetics constant during storage after physical damage.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4


  1. Angersbach, A., Heinz, V., & Knorr, D. (1999). Electrophysiological model of intact and processed plant tissues: cell disintegration criteria. Biotechnology Progress, 15(4), 753–762.

  2. Cubero, S., Lee, W. S., Aleixos, N., Albert, F., & Blasco, J. (2016). Automated systems based on machine vision for inspecting citrus fruits from the field to postharvest—a review. Food and Bioprocess Technology, 9(10), 1623–1639.

  3. Fava, J., Hodara, K., Nieto, A., Guerrero, S., Alzamora, S. M., & Castro, M. A. (2011). Structure (micro, ultra, nano), color and mechanical properties of Vitis labrusca L. (grape berry) fruits treated by hydrogen peroxide, UV–C irradiation and ultrasound. Food Research International, 44(9), 2938–2948.

  4. Feng, L., Zhang, M., Bhandari, B., & Guo, Z. (2018). Determination of postharvest quality of cucumbers using nuclear magnetic resonance and electronic nose combined with chemometric methods. Food and Bioprocess Technology, 11(12), 2142–2152.

  5. Hayden, R. I., Moyse, C. A., Calder, R. W., Crawford, D. P., & Fensom, D. S. (1969). Electrical impedance studies on potato and alfalfa tissue. Journal of Experimental Botany, 20(63), 177–200.

  6. Imaizumi, T., Tanaka, F., Hamanaka, D., Sato, Y., & Uchino, T. (2015). Effects of hot water treatment on electrical properties, cell membrane structure and texture of potato tubers. Journal of Food Engineering, 162, 56–62.

  7. Lee, Y., Watanabe, T., Nakaura, Y., Ando, Y., Nagata, M., & Yamamoto, K. (2019). Cultivar differences in electrical and mechanical property changes and tolerance in apples due to high hydrostatic pressure treatment. Postharvest Biology and Technology, 156, 110947.

  8. Martinez-Romero, D., Serrano, M., Carbonell, A., Burgos, L., Riquelme, F., & Valero, D. (2002). Effects of postharvest putrescine treatment on extending shelf life and reducing mechanical damage in apricot. Journal of Food Science, 67(5), 1706–1712.

  9. Matsumoto, H., & Ikoma, Y. (2016). Effect of postharvest temperature on the Muscat flavor and aroma volatile content in the berries of “Shine Muscat” (Vitis labruscana Baily × V. vinifera L.). Postharvest Biology and Technology, 112, 256–265.

  10. Meng, X., Li, B., Liu, J., & Tian, S. (2008). Physiological responses and quality attributes of table grape fruit to chitosan preharvest spray and postharvest coating during storage. Food Chemistry, 106(2), 501–508.

  11. Ministry of Agriculture, Forestry and Fisheries. (2018).,

  12. Mohsenin, N. N. (1970). Physical properties of plant and animal materials. Vol. 1. Structure, physical characteristics and mechanical properties. Physical properties of plant and animal materials. Vol. 1. Structure, physical characteristics and mechanical properties., 1.

  13. Opara, U. L., & Pathare, P. B. (2014). Bruise damage measurement and analysis of fresh horticultural produce—a review. Postharvest Biology and Technology, 91, 9–24.

  14. Palou, L., Crisosto, C. H., Garner, D., & Basinal, L. M. (2003). Effect of continuous exposure to exogenous ethylene during cold storage on postharvest decay development and quality attributes of stone fruits and table grapes. Postharvest Biology and Technology, 27(3), 243–254.

  15. Pathare, P. B., Opara, U. L., & Al-Said, F. A. J. (2013). Colour measurement and analysis in fresh and processed foods: a review. Food and Bioprocess Technology, 6(1), 36–60.

  16. Shin, Y., Ryu, J. A., Liu, R. H., Nock, J. F., & Watkins, C. B. (2008). Harvest maturity, storage temperature and relative humidity affect fruit quality, antioxidant contents and activity, and inhibition of cell proliferation of strawberry fruit. Postharvest Biology and Technology, 49(2), 201–209.

  17. Watanabe, T., Nakamura, N., Ando, Y., Kaneta, T., Kitazawa, H., & Shiina, T. (2018a). Application and simplification of cell-based equivalent circuit model analysis of electrical impedance for assessment of drop shock bruising in Japanese pear tissues. Food and Bioprocess Technology, 11(11), 2125–2129.

  18. Watanabe, T., Nakamura, N., Ota, N., & Shiina, T. (2018b). Estimation of changes in mechanical and color properties from the weight loss data of “Shine Muscat” fruit during storage. Journal of Food Quality, art. no. 7258029 (6 pages).

  19. Watanabe, T., Ando, Y., Orikasa, T., Kasai, S., & Shiina, T. (2018c). Electrical impedance estimation for apple fruit tissues during storage using Cole–Cole plots. Journal of Food Engineering, 221, 29–34.

  20. Wu, L., Ogawa, Y., & Tagawa, A. (2008). Electrical impedance spectroscopy analysis of eggplant pulp and effects of drying and freezing–thawing treatments on its impedance characteristics. Journal of Food Engineering, 87(2), 274–280.

  21. Yang, D., Li, D., Xu, W., Fu, Y., Wu, F., Liao, R., & Wang, J. (2018). Effects of packaging design with dual function films on quality of wax apples stored at ambient temperatures. Food and Bioprocess Technology, 11(10), 1904–1910.

  22. Zhang, M. I. N., & Willison, J. H. M. (1992). Electrical impedance analysis in plant tissues: the effect of freeze-thaw injury on the electrical properties of potato tuber and carrot root tissues. Canadian Journal of Plant Science, 72(2), 545–553.

Download references


This study was supported by the Kieikai Research Foundation.

Author information

Correspondence to Takashi Watanabe.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Watanabe, T. A Bio-Electrochemical Calculation Model for Color Decline Kinetics of Bruised “Shine Muscat” Fruit During Storage. Food Bioprocess Technol (2020).

Download citation


  • “Shine Muscat”
  • Electrical impedance estimation
  • Physical damage
  • Color change kinetics