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The Mediation of NO-Enhanced Chilling Tolerance by GSK-3 in Postharvest Peach Fruit

  • Caifeng Jiao
  • Yuquan DuanEmail author
Original Paper
  • 44 Downloads

Abstract

The role of glycogen synthase kinase-3 (GSK-3) in nitric oxide (NO)-enhanced chilling tolerance in postharvest peach fruit was investigated. The fruits were immersed in sodium nitroprusside (SNP; exogenous NO donor) and bikinin (GSK-3 inhibitor). Results showed that the chilling injury (CI) index declined following the exposure of the peach fruit to exogenous SNP. SNP treatment also induced GSK-3 expression. Furthermore, SNP treatment reduced malondialdehyde (MDA) content and electrolyte leakage in the peach fruit. In addition, SNP treatment induced the increase in alternative oxidase (AOX) activity and the upregulation of the gene expression of 18.1-kDa class I heat shock protein (HSP), WRKY2, and C-repeat binding factor (CBF). The effects of SNP treatment were partly weakened by the addition of bikinin. These findings indicate that GSK-3 mediated the reduction of MDA content and electrolyte leakage and the activation of AOX, 18.1-kDa class I HSP, WRKY2, and CBF by NO, thereby inducing chilling tolerance in peach fruit.

Keywords

SNP GSK-3 MDA and electrolyte leakage AOX HSP, WRKY and CBF Peach fruit 

Abbreviations

GSK-3

Glycogen synthase kinase-3

NO

Nitric oxide

SNP

Sodium nitroprusside

CI

Chilling injury

MDA

Malondialdehyde

AOX

Alternative oxidase

HSP

Heat shock protein

CBF

C-Repeat binding factor

Notes

Funding Information

This project was supported by the National Natural Science Foundation of China (31871862).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport ProcessMinistry of Agriculture and Rural AffairsBeijingPeople’s Republic of China

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