Novel Role of Ethanol in Delaying Postharvest Physiological Deterioration and Keeping Quality in Cassava
- 105 Downloads
Massive economic losses and the decrease in quality of cassava are caused by postharvest physiological deterioration (PPD). However, an effective solution remains limited. In this study, the role of ethanol in the PPD of cassava was investigated and highlighted. Exogenous ethanol delayed PPD and reduced the accumulation of reactive oxygen species, while increased the underlying activities of superoxide dismutase, catalase, peroxidase, and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging. Moreover, exogenous ethanol increased the endogenous levels of ethylene and melatonin, all of which are negative regulators of PPD. Notably, this study found that exogenous ethanol reduced the degradation of starch, but enhanced ascorbic acid content and carotenoid content. In summary, these results revealed the novel role of ethanol in delaying PPD and improving the quality of cassava tubes without ethanol residue, suggesting an effective and promising way in cassava.
KeywordsCassava Ethanol Ethylene Postharvest physiological deterioration Quality
This research was supported by the Scientific Research Project of Higher Education in Hainan Education Department (No. Hnky2018-7) to Guoyin Liu, the startup funding, and the scientific research foundation of the Hainan University (No. kyqd1531) to Haitao Shi, and the crop science postgraduate innovation project of Hainan university tropical agriculture and forestry college (No.ZWCX2018017) to Bing Li.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- Ding, Y., Zhu, Z., Zhao, J., Nie, Y., Zhang, Y., Sheng, J., Meng, D., Mao, H., & Tang, X. (2016). Effects of postharvest brassinolide treatment on the metabolism of white button mushroom (Agaricus bisporus) in relation to development of browning during storage. Food and Bioprocess Technology, 9(8), 1327–1334.CrossRefGoogle Scholar
- Gao, J. F. (2007). Experimental guidance plant physiology (pp. 145–225). Beijing: Higher Education Press.Google Scholar
- Hattori, A., Migitaka, H., Iigo, M., Itoh, M., Yamamoto, K., Ohtani-Kaneko, R., Hara, M., Suzuki, T., & Reiter, R. J. (1995). Identification of melatonin in plants and its effects on plasma melatonin levels and binding to melatonin receptors in vertebrates. Biochemistry and Molecular Biology International, 35(3), 627–634.Google Scholar
- Hu, W., Kong, H., Guo, Y., Zhang, Y., Ding, Z., Tie, W., Yan, Y., Huang, Q., Peng, M., Shi, H., & Guo, A. (2016). Comparative physiological and transcriptomic analyses reveal the actions of melatonin in the delay of postharvest physiological deterioration of cassava. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2016.00736.
- Hu, W., Tie, W., Ou, W., Yan, Y., Kong, H., Zuo, J., Ding, X., Ding, Z., Liu, Y., Wu, C., Guo, Y., Shi, H., Li, K., & Guo, A. (2018). Crosstalk between calcium and melatonin affects postharvest physiological deterioration and quality loss in cassava. Postharvest Biology and Technology, 140, 42–49.CrossRefGoogle Scholar
- Li, M. L., Li, X., Li, J., Ji, Y., Han, C., Jin, P., & Zheng, Y. (2018). Responses of fresh-cut strawberries to ethanol vapor pretreatment: improved quality maintenance and associated antioxidant metabolism in gene expression and enzyme activity levels. Journal of Agricultural and Food Chemistry, 66(31), 8382–8390.CrossRefGoogle Scholar
- Liu, W. W., Qi, H., Xu, B., Li, Y., Tian, X., Jiang, Y., Xu, X., & Lv, D. (2012). Ethanol treatment inhibits internal ethylene concentrations and enhances ethyl ester production during storage of oriental sweet melons (Cucumis melo var. makuwa Makino). Postharvest Biology and Technology, 67, 75–83.CrossRefGoogle Scholar
- Moriwaki, T., Yamamoto, Y., Aida, T., Funahashi, T., Shishido, T., Asada, M., Prodhan, S., Komamine, A., & Motohashi, T. (2008). Overexpression of the escherichia coli catalase gene, katE, enhances tolerance to salinity stress in the transgenic indica rice cultivar, BR5. Plant Biotechnology Reports, 2(1), 41–46.CrossRefGoogle Scholar
- Saravanan, R., Ravi, V., Stephen, R., Thajudhin, S., & George, J. (2016). Post-harvest physiological deterioration of cassava (Manihot esculenta) − a review. Indian Journal Agriculture Science, 86(11), 1383–1390.Google Scholar
- Shi, H., & Chan, Z. (2014). The cysteine2/histidine2-type transcription factor ZINC FINGER OF ARABIDOPSIS THALIANA 6-activated C-REPEAT-BINDING FACTOR pathway is essential for melatonin-mediated freezing stress resistance in Arabidopsis. Journal of Pineal Research, 57(2), 185–191.CrossRefGoogle Scholar
- Tan, D. X., Chen, L. D., Poeggeler, B., Manchester, L. C., & Reiter, R. J. (1993). Melatonin: a potent, endogenous hydroxyl radical scavenger. Endocrine Journal, 1, 57–60.Google Scholar
- Uarrota, V. G., Costa Nunes, E., Peruch, L. A. M., Oliveira Neubert, E., Coelho, B., Moresco, R., Domínguez, M., Sánchez, T., Meléndez, J., Dufour, D., Ceballos, H., Lopez-Lavalle, L., Hershey, C., Rocha, M., & Maraschin, M. (2016). Toward better understanding of postharvest deterioration: biochemical changes in stored cassava (Manihot esculenta Crantz) roots. Food Science Nutrition, 4(3), 409–422.CrossRefGoogle Scholar
- Vanderschuren, H., Nyaboga, E., Poon, J. S., Baerenfaller, K., Grossmann, J., Hirsch-Hoffmann, M., Kirchgessner, N., Nanni, P., & Gruissem, W. (2014). Large-scale proteomics of the cassava storage root and identification of a target gene to reduce postharvest deterioration. Plant Cell, 26(5), 1913–1924.CrossRefGoogle Scholar
- Wei, Y., Hu, W., Wang, Q., Liu, W., Wu, C., Zeng, H., Yan, Y., Li, X., He, C., & Shi, H. (2016). Comprehensive transcriptional and functional analyses of melatonin synthesis genes in cassava reveal their novel role in hypersensitive-like cell death. Scientific Reports, 6, 35029.CrossRefGoogle Scholar