Food and Bioprocess Technology

, Volume 12, Issue 10, pp 1708–1720 | Cite as

Optimization of Pulsed Electric Fields-Assisted Extraction of Polyphenols from Potato Peels Using Response Surface Methodology

  • D. Frontuto
  • D. Carullo
  • S. M. Harrison
  • N. P. Brunton
  • G. Ferrari
  • J. G. Lyng
  • G. PataroEmail author
Original Paper


In this work, optimal pulsed electric fields-assisted extraction conditions were selected in order to intensify the extractability of polyphenol compounds with high antioxidant activity from potato peels. Effectiveness of PEF as cell disintegration technique was confirmed using both impedance measurements and scanning electron microscopy (SEM). Solid-liquid extraction (SLE) for both untreated and PEF pre-treated potato peels was optimized to determine the most effective solvent concentration (0–100% ethanol in water) as well as extraction temperature (20–50 °C) and time (30–240 min) using response surface methodology. Total phenolic compounds (TPC) and antioxidant activity (DPPH) of the extracts were determined. Results showed that the application of PEF prior to SLE has the potential to reduce duration, temperature, and consumption of solvent to achieve the same recovery yield of phenolic compounds. Under optimized conditions (54% ethanol, 233 min, and 50 °C for SLE; 52% ethanol, 230 min, and 50 °C for PEF), the extracts obtained from PEF pre-treated samples showed higher total phenolics yield (10%) and antioxidant activity (9%) as compared to the control extraction. Finally, HPLC-DAD analysis revealed the major classes of the detected polyphenolic compounds as chlorogenic, caffeic, syringic, protocatechuic, and p-coumaric acids, and no significant degradation of individual polyphenols due to PEF application was observed.


Pulsed electric fields (PEF) Solid-liquid extraction (SLE) Potato peels by-product Polyphenols HPLC Response surface methodology 



  1. Akyol, H., Riciputi, Y., Capanoglu, E., Caboni, M. F., & Verardo, V. (2016). Phenolic compounds in the potato and its byproducts: an overview. International Journal of Molecular Sciences, 17(6).
  2. Amado, I. R., Franco, D., Sánchez, M., Zapata, C., & Vázquez, J. A. (2014). Optimisation of antioxidant extraction from Solanum tuberosum potato peel waste by surface response methodology. Food Chemistry, 165, 290–299.CrossRefGoogle Scholar
  3. Anastácio, A., & Carvalho, I. S. (2013). Phenolics extraction from sweet potato peels: key factors screening through a Placket–Burman design. Industrial Crops and Products, 43, 99–105.CrossRefGoogle Scholar
  4. Asavasanti, S., Ersus, S., Ristenpart, W., Stroeve, P., & Barrett, D. M. (2010). Critical electric field strengths of onion tissues treated by pulsed electric fields. Journal of Food Science, 75(7), E433–E443.CrossRefGoogle Scholar
  5. Barba, F. J., Brianceau, S., Turk, M., Boussetta, N., & Vorobiev, E. (2015). Effect of alternative physical treatments (ultrasounds, pulsed electric fields, and high-voltage electrical discharges) on selective recovery of bio-compounds from fermented grape pomace. Food & Bioprocess Technology, 8(5), 1139–1148.CrossRefGoogle Scholar
  6. Barbosa-Pereira, L., Guglielmetti, A., & Zeppa, G. (2018). Pulsed electric field assisted extraction of bioactive compounds from cocoa bean shell and coffee silverskin. Food & Bioprocess Technology, 11(4), 818–835.CrossRefGoogle Scholar
  7. Bobinaitė, R., Pataro, G., Lamanauskas, N., Šatkauskas, S., Viškelis, P., & Ferrari, G. (2015). Application of pulsed electric field in the production of juice and extraction of bioactive compounds from blueberry fruits and their by-products. Journal of Food Science and Technology, 52(9), 5898–5905.CrossRefGoogle Scholar
  8. Boussetta, N., Vorobiev, E., Le, L., Cordin-Falcimaigne, A., & Lanoisellé, J.-L. (2012). Application of electrical treatments in alcoholic solvent for polyphenols extraction from grape seeds. LWT-Food Science and Technology, 46(1), 127–134.CrossRefGoogle Scholar
  9. Carullo, D., Abera, B. D., Casazza, A. A., Donsì, F., Perego, P., Ferrari, G., & Pataro, G. (2018). Effect of pulsed electric fields and high pressure homogenization on the aqueous extraction of intracellular compounds from the microalgae Chlorella vulgaris. Algal Research, 31, 60–69.CrossRefGoogle Scholar
  10. Chang, K. (2011). Polyphenol antioxidants from potato peels: extraction optimization and application to stabilizing lipid oxidation in foods. In Proceedings of the National Conference on Undergraduate Research (NCUR). New York, NY, USA.Google Scholar
  11. Donsì, F., Ferrari, G., & Pataro, G. (2010). Applications of pulsed electric field treatments for the enhancement of mass transfer from vegetable tissue. Food Engineering Reviews, 2(2), 109–130.CrossRefGoogle Scholar
  12. El Gharras, H. (2009). Polyphenols: food sources, properties and applications - a review. International Journal of Food Science and Technology, 44(12), 2512–2518.CrossRefGoogle Scholar
  13. FAOSTAT (2017) Data of crops production in the World and Europe. Data Division. Available online: Accessed 01 April 2019.
  14. Friedman, M. (1997). Chemistry, biochemistry, and dietary role of potato polyphenols. A review. Journal of Agricultural and Food Chemistry, 45(5), 1523–1540.CrossRefGoogle Scholar
  15. Galanakis, C. M. (2012). Recovery of high added-value components from food wastes: conventional, emerging technologies and commercialized applications. Trends in Food Science & Technology, 26(2), 68–87.CrossRefGoogle Scholar
  16. Hossain, M. B., Tiwari, B. K., Gangopadhyay, N., O’Donnell, C. P., Brunton, N. P., & Rai, D. K. (2014). Ultrasonic extraction of steroidal alkaloids from potato peel waste. Ultrasonics Sonochemistry, 21(4), 1470–1476.CrossRefGoogle Scholar
  17. Huang, H. W., Hsu, C. P., Yang, B. B., & Wang, C. Y. (2013). Advances in the extraction of natural ingredients by high pressure extraction technology. Trends in Food Science & Technology, 33(1), 54–62.CrossRefGoogle Scholar
  18. Kanatt, S. R., Chander, R., Radhakrishna, P., & Sharma, A. (2005). Potato peel extract a natural antioxidant for retarding lipid peroxidation in radiation processed lamb meat. Journal of Agricultural and Food Chemistry, 53(5), 1499–1504.CrossRefGoogle Scholar
  19. Lebovka, N., Bazhal, M., & Vorobiev, E. (2002). Estimation of characteristic damage time of food materials in pulsed-electric fields. Journal of Food Engineering, 54(4), 337–346.CrossRefGoogle Scholar
  20. López, N., Puértolas, E., Hernández-Orte, P., Álvarez, I., & Raso, J. (2009). Effect of a pulsed electric field treatment on the anthocyanins composition and other quality parameters of cabernet sauvignon freshly fermented model wines obtained after different maceration times. LWT-Food Science and Technology, 42(7), 1225–1231.CrossRefGoogle Scholar
  21. Luengo, E., Álvarez, I., & Raso, J. (2013). Improving the pressing extraction of polyphenols of orange peel by pulsed electric fields. Innovative Food Science & Emerging Technologies, 17, 79–84.CrossRefGoogle Scholar
  22. Luengo, E., Álvarez, I., & Raso, J. (2014). Improving carotenoid extraction from tomato waste by pulsed electric fields. Frontiers in Nutrition, 1, 1–10.CrossRefGoogle Scholar
  23. Onyeneho, S. N., & Hettiarachchy, N. S. (1993). Antioxidant activity, fatty acids and phenolic acids compositions of potato peels. Journal of the Science of Food and Agriculture, 62(4), 345–350.CrossRefGoogle Scholar
  24. Paleologou, I., Vasiliou, A., Grigorakis, S., & Makris, D. P. (2016). Optimisation of a green ultrasound-assisted extraction process for potato peel (Solanum tuberosum) polyphenols using bio-solvents and response surface methodology. Biomass Conversion and Biorefinery, 6(3), 289–299.CrossRefGoogle Scholar
  25. Parniakov, O., Barba, F. J., Grimi, N., Lebovka, N., & Vorobiev, E. (2014). Impact of pulsed electric fields and high voltage electrical discharges on extraction of high-added value compounds from papaya peels. Food Research International, 65, 337–343.CrossRefGoogle Scholar
  26. Parniakov, O., Barba, F. J., Grimi, N., Lebovka, N., & Vorobiev, E. (2016). Extraction assisted by pulsed electric energy as a potential tool for green and sustainable recovery of nutritionally valuable compounds from mango peels. Food Chemistry, 192, 842–848.CrossRefGoogle Scholar
  27. Pataro, G., Ferrari, G., & Donsì, F. (2011). Mass transfer enhancement by means of electroporation. In J. Markoš (Ed.), Mass transfer in chemical engineering processes (pp. 151–176). Rijeka: InTech ISBN 978-953-307-619-5.Google Scholar
  28. Pataro, G., Bobinaitė, R., Bobinas, Č., Šatkauskas, S., Raudonis, R., Visockis, M., Ferrari, G., & Viškelis, P. (2017). Improving the extraction of juice and anthocyanins from blueberry fruits and their by-products by application of pulsed electric fields. Food Bioprocess Technology, 10(9), 1595–1605. Scholar
  29. Pataro, G., Carullo, D., Siddique, M. A. B., Falcone, M., Donsì, F., & Ferrari, G. (2018). Improved extractability of carotenoids from tomato peels as side benefits of PEF treatment of tomato fruit for more energy-efficient steam-assisted peeling. Journal of Food Engineering, 233, 65–73.CrossRefGoogle Scholar
  30. Patra, M., Salonen, E., Terama, E., Vattulainen, I., Faller, R., Lee, B. W., Holopainen, J., & Karttunen, M. (2006). Under the influence of alcohol: the effect of ethanol and methanol on lipid bilayers. Biophysical Journal, 90(4), 1121–1135.CrossRefGoogle Scholar
  31. Pillet, F., Formosa-Dague, C., Baaziz, H., Dague, E., & Rols, M. P. (2016). Cell wall as a target for bacteria inactivation by pulsed electric fields. Scientific Reports, 6, 19778. Scholar
  32. Puértolas, E., Cregenzán, O., Luengo, E., Álvarez, I., & Raso, J. (2013). Pulsed-electric-field-assisted extraction of anthocyanins from purple-fleshed potato. Food Chemistry, 136(3-4), 1330–1336.CrossRefGoogle Scholar
  33. Rapisarda, P., Tomaino, A., Lo Cascio, R., Bonina, F., De Pasquale, A., & Saija, A. (1999). Antioxidant effectiveness as influenced by phenolic content of fresh orange juices. Journal of Agricultural and Food Chemistry, 47(11), 4718–4723.CrossRefGoogle Scholar
  34. Roselló-Soto, E., Parniakov, O., Deng, Q., Patras, A., Koubaa, M., Grimi, N., Boussetta, N., Tiwari, B. K., Vorobiev, E., Lebovka, N., & Barba, F. J. (2016). Application of non-conventional extraction methods: toward a sustainable and green production of valuable compounds from mushrooms. Food Engineering Reviews, 8(2), 214–234.CrossRefGoogle Scholar
  35. Samarin, A. M., Poorazarang, H., Hematyar, N., & Elhamirad, A. (2012). Phenolics in potato peels: extraction and utilization as natural antioxidants. World Applied Sciences Journal, 18, 191–195.Google Scholar
  36. Schieber, A., & Saldaña, M. D. (2009). Potato peels: a source of nutritionally and pharmacologically interesting compounds-a review. Food, 3, 23–29.Google Scholar
  37. Singh, P. P., & Saldaña, M. D. (2011). Subcritical water extraction of phenolic compounds from potato peel. Food Research International, 44(8), 2452–2458.CrossRefGoogle Scholar
  38. Wijngaard, H. H., Ballay, M., & Brunton, N. (2012). The optimisation of extraction of antioxidants from potato peel by pressurised liquids. Food Chemistry, 133(4), 1123–1130.CrossRefGoogle Scholar
  39. Wu, T., Yan, J., Liu, R., Marcone, M. F., Aisa, H. A., & Tsao, R. (2012). Optimization of microwave-assisted extraction of phenolics from potato and its downstream waste using orthogonal array design. Food Chemistry, 133(4), 1292–1298.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.UCD Institute of Food and Health, UCDDublin 4Ireland
  2. 2.Department of Industrial EngineeringUniversity of SalernoFiscianoItaly
  3. 3.ProdAl Scarl – University of SalernoFiscianoItaly

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