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Study of Static Steam Explosion of Citrus sinensis Juice Processing Waste for the Isolation of Sugars, Pectic Hydrocolloids, Flavonoids, and Peel Oil

  • Christina DoradoEmail author
  • Randall G. Cameron
  • John A. Manthey
Original Paper

Abstract

In this work, citrus juice processing waste (CPW) from a local Florida processor was subjected to steam explosion using a static bench scale reactor at 130, 150, and 170 °C for 1, 2, 4, and 8 min hold times with the aim of identifying conditions necessary for maximizing the recovery of sugars, pectic hydrocolloids, flavonoids, and peel oil. Sugars in steam-exploded CPW, from two harvests each of Hamlin and Valencia oranges, were extracted using water or enzymatic hydrolysis. Hydrolysis resulted in a 32–39% increase in total sugars. While hydrolysis increased the amount of glucose or fructose, it did not necessarily reduce the temperatures and or hold times at which the maximum amounts of sugar were recovered. Peel oil in raw CPW was reduced by as much as 94% using steam explosion and has the potential to be recovered by condensation. More galacturonic acid was recovered at 150 and 170 °C and 1 min treatment time for both Hamlin and Valencia CPW but led to increased molecular weight, suspected to be caused by aggregation, and decreased intrinsic viscosity. The higher temperatures and hold times also allowed vastly improved recoveries of the otherwise insoluble flavanone glycoside, hesperidin, with a maximum yield of 64,611 ppm dw CPW. Other potential value-added materials such as hydroxycinnamates and the health-benefiting polymethoxylated flavones are also recoverable in high percent yields in the water washes after steam explosion. Estimates are provided of the value of these recoverable products in the CPW of the 2016–17 Florida citrus season.

Keywords

STEX Pectin Glucose Fructose Sucrose Hesperidin 

Notes

Acknowledgements

The authors would like to express their gratitude to Sandra Matlack, PeiLing Li, and Veronica Cook for providing technical support for experiments and analyses. The authors would also like to thank Justice Widman for providing technical support for peel oil analyses.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11947_2019_2300_MOESM1_ESM.docx (1.4 mb)
ESM 1 (DOCX 1.40 mb)

References

  1. Alam-Eldein, S., Albrigo, G., Rouseff, R., & Tubeileh, A. (2017). Characterization of citrus peel maturation: Multivariate and multiple regression analyses. Acta Horticulturae, 1160, 349–356.CrossRefGoogle Scholar
  2. Anonymous (2018). Glycoproducts for life sciences - engineering and production. https://www.elicityl-oligotech.com/. Accessed 28 Aug 2018.
  3. Bailey, A., Leong, J., & Fitzgerald, N. (2015). Bioproducts to enable biofuels workshop summary report. Westminster: U.S. Department of Energy, Office of Energy Efficiency & Renewable Energy.CrossRefGoogle Scholar
  4. Boulda-Aguilar, M., & Lopez-Gomez, A. (2013). Production of bioethanol by fermentation of lemon (Citrus limon L.) peel wastes pretreated with steam explosion. Industrial Crops and Products, 41, 188–197.CrossRefGoogle Scholar
  5. Bozell, J. J., & Petersen, G. R. (2010). Technology Development for the production of biobased products from biorefinery carbohydrates-the US Department of Energy’s “top 10” revisted. Green Chemistry, 12(4), 539–554.CrossRefGoogle Scholar
  6. Braddock, R. J. (1999). Handbook of Citrus by-products and processing technology. New York: John Wiley & Sons.Google Scholar
  7. Burns, J. K., & Baldwin, E. A. (1994). Glycosidase activities in grapefruit flavedo, albedo and juice vesicles during maturation and senescence. Physiologia Plantarum, 90(1), 37–44.CrossRefGoogle Scholar
  8. Cameron, R. G., Chau, H. K., & Manthey, J. A. (2016). Continuous process for enhanced release and recovery of pectic hydrocolloids and phenolics from citrus biomass. Journal of Chemical Technology and Biotechnology, 91(10), 2597–2606.CrossRefGoogle Scholar
  9. Cameron, R. G., Chau, H. K., Hotchkiss, A. T., & Manthey, J. A. (2017). Release and recovery of pectic hydrocolloids and phenolics from culled citrus fruits. Food Hydrocolloids, 72, 52–61.CrossRefGoogle Scholar
  10. de Jong, E., Higson, A., Walsh, P., & Wellisch, M. (2012). Bio-based chemicals: value added products from biorefineries, international energy agency, bioenergy task 42: Biorefinery. Amsterdam, The Netherlands.Google Scholar
  11. FCM (2017). Florida Citrus mutual, glossary. Metric tons were calculated from the conversion of one field box being equivalent to 90 pounds of oranges. http://flcitrusmutual.com/citrus-101/glossary.aspx. Accessed 7 April 2017.
  12. FDOC (2017). Florida Department of Citrus, 2015–2016 Annual Processors Statistics Report. https://app.box.com/shared/6erp573bkl/1/76180930/133491859850/1. Accessed 7 April 2017.
  13. Fishman, M. L., Chau, H. K., Hoagland, P., & Ayyad, K. (2000). Characterization of pectin, flash-extracted from orange albedo by microwave heating, under pressure. Carbohydrate Research, 323(1–4), 126–138.Google Scholar
  14. Fishman, M. L., Walker, P. N., Chau, H. K., & Hotchkiss, A. T. (2003). Flash extraction of pectin from orange albedo by steam injection. Biomacromolecules, 4(4), 880–889.CrossRefGoogle Scholar
  15. Fishman, M. L., Chau, H. K., Hoagland, P. D., & Hotchkiss, A. T. (2006). Microwave-assisted extraction of lime pectin. Food Hydrocolloids, 20(8), 1170–1177.CrossRefGoogle Scholar
  16. Gelski, J. (2016). Food business news. https://www.foodbusinessnews.net/articles/8174-a-squeeze-on-pectin. Accessed 27 Aug 2018.
  17. Goodrich, R.M., & Braddock, R. J. (2004). University of Florida Institute of food and agricultural sciences extension. Major By-Products of the Florida Citrus Processing Industry. http://ufdcimages.uflib.ufl.edu/IR/00/00/20/62/00001/FS10700.pdf. Accessed 10 April 2017.
  18. Grohmann, K., Cameron, R. G., & Buslig, B. S. (1995). Fractionation and pretreatment of orange peel by dilute acid hydrolysis. Bioresource Technology, 54(2), 129–141.CrossRefGoogle Scholar
  19. Grohmann, K., Cameron, R., Kim, Y., Widmer, W., & Luzio, G. (2013). Extraction and recovery of pectic fragments from citrus processing waste for co-production with ethanol. Journal of Chemical Technology and Biotechnology, 88(3), 395–407.CrossRefGoogle Scholar
  20. Kertesz, Z.I. (1951). The pectic substances, 1st Ed. New York, NY: Interscience Publishers.Google Scholar
  21. Kesterson, J. W., & Hendrickson, R. (1958). Utilization of citrus by-products. Economic Botany, 12(2), 164–185.CrossRefGoogle Scholar
  22. Kimball, D. (1991). Citrus processing quality control and technology. New York: AVI/Van Nostrand Reinhold.CrossRefGoogle Scholar
  23. Maric, M., Grassino, A. N., Zhu, Z., Barba, F. J., Brncic, M., & Brncic, S. R. (2018). An overview of the traditional and innovative approaches for pectinextraction from plant food wastes and by-products: Ultrasound-,microwaves-, and enzyme-assisted extraction. Trends in Food Science & Technology, 76, 28–37.CrossRefGoogle Scholar
  24. NASS (2017a). National Agricultural Statistics Service, 2017 Citrus Fruits Summary. http://usda.mannlib.cornell.edu/usda/current/CitrFrui/CitrFrui-08-31-2017.pdf. Accessed 9 April 2018.
  25. NASS (2017b). National Agricultural Statistics Service, 2017 State Agriculture Overview (Florida). https://www.nass.usda.gov/Quick_Stats/Ag_Overview/stateOverview.php?state=FLORIDA. Accessed 9 April 2018.
  26. NASS (2018). National Agricultural Statistics Service, Florida Citrus Statistics 2016–2017. https://www.nass.usda.gov/Statistics_by_State/Florida/Publications/Citrus/Citrus_Statistics/2016-17/fcs1617.pdf. Accessed 16 July 2018.
  27. Negro, V., Mancini, G., Ruggeri, B., & Fino, D. (2016). Citrus waste as feedstock for bio-based products recovery: Review on limonene case study and energy valorization. Bioresource Technology, 214, 806–815.CrossRefGoogle Scholar
  28. Rafiq, S., Kaul, R., Sofi, S. A., Bashir, N., Nazir, F., & Nayik, G. A. (2018). Citrus peel as a source of functional ingredient: A review. Journal of the Saudi Society of Agricultural Sciences, 17(4), 351–358.CrossRefGoogle Scholar
  29. Rezzoug, S.-A., & Louka, N. (2009). Thermochemical process intensification for oil extraction from orange peels. Innovative Food Science and Emerging Technologies, 10(4), 530–536.CrossRefGoogle Scholar
  30. Rolin, C. (2002). Commercial pectin preparations (review article). Pectins and their Manipulation. G.B. and J.P. Knox (Eds.) Oxford, Great Britain: Blackwell Publishing, pp. 222–241.Google Scholar
  31. Rouse, A. H. (1953). Distribution of pectinesterase and total pectin in component parts of citrus fruits. Food Technology, 7(9), 360–362.Google Scholar
  32. Sharma, K., Mahato, N., Cho, M. H., & Lee, Y. R. (2017). Converting citrus wastes into value-added products: Economic and environmentally friendly approaches. Nutrition, 34, 29–46.CrossRefGoogle Scholar
  33. Shaw, P. E., Calkins, C. O., McDonald, R. E., Greany, P. D., Webb, J. C., Nisperos-Carriedo, M. O., & Barros, S. M. (1991). Changes in limonin and naringin levels in grapefruit albedo with maturity and the effects of gibberellic acid on these changes. Phytochemistry, 30(10), 3215–3219.CrossRefGoogle Scholar
  34. Straathof, A. J. J., & Bampouli, A. (2017). Potential of commodity chemicals to become bio-based according to maximum yields and petrochemical prices. Biofuels, Bioproducts and Biorefining, 11(5), 798–810.CrossRefGoogle Scholar
  35. Sweet Orange Essential oil with 90% D-limonene Cold Pressed Sweet Orange Oil (2018) Alibaba.com. https://www.alibaba.com/product-detail/Sweet-Orange-Essential-oil-with-90_60645336815.html?spm=a2700.7724857.main07.259.7d481c23je005y. Accessed 20 April 2018.
  36. Tucker, D. P. H., & Long, S. K. (1968). More by-products research needed. Citrus World, 4, 13–17.Google Scholar
  37. Vennestrøm, P. N., Osmundsen, C. M., Christensen, C. H., & Taarning, E. (2011). Beyond petrochemicals: The renewable chemicals industry. Angewandte Chemie, International Edition, 50, 10502 – 10509.CrossRefGoogle Scholar
  38. Wegener, S., Bornik, M.-A., & Kroh, L. W. (2015). d-Galacturonic acid: A highly reactive compound in nonenzymatic browning. 2. Formation of amino-specific degradation products. Journal of Agriculture and Food Chemistry, 63(28), 6457–6465.CrossRefGoogle Scholar
  39. Werpy, T., & Petersen, G. (Eds.). (2004). Top Value Added Chemical from Biomass Volume I-Results of Screening for Potential Candidates from Sugars and Synthesis Gas. United States Department of Energy, Office of Energy, efficiency and renewable energy. Springfield, Virginia.Google Scholar
  40. Widmer, W. (2011). Analysis of biomass sugars and galacturonic acid by gradient anion exchange chromatography and pulsed amperometric detection without post-column addition. Biotechnology Letters, 33(2), 365–368.CrossRefGoogle Scholar
  41. Widmer, W. W., & Stewart, D. (2006). Ethanol from citrus peel waste. Industrial Bioprocessing, 28(5), 5.Google Scholar
  42. Widmer, W., Stewart, D., Grohmann, K., & Wilkins, M. (2006). Method of pretreating citrus waste. US Patent 7,879,379.Google Scholar
  43. Widmer, W. W., Narciso, J. A., Grohmann, K., & Wilkins, M. R. (2009). Simultaneous saccharification and fermentation of orange processing waste to ethanol using Kluyveromyces marxianus. Biological Engineering, 2(1), 17–29.CrossRefGoogle Scholar
  44. Widmer, W., Zhou, W., & Grohmann, K. (2010). Pretreatment effects on orange processing waste for making ethanol by simultaneous saccharification and fermentation. Bioresource Technology, 101(14), 5242–5249.CrossRefGoogle Scholar
  45. Wilkins, M. R., Widmer, W. W., & Grohmann, K. (2007). Simultaneous saccharification and fermentation of citrus peel waste by Saccharomyces cerevisiae to produce ethanol. Process Biochemistry, 42(12), 1614–1619.CrossRefGoogle Scholar

Copyright information

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2019

Authors and Affiliations

  1. 1.U.S. Department of Agriculture, Agricultural Research Service, U.S. Horticultural Research LaboratoryFt. PierceUSA

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