Production of Whey-Derived DPP-IV Inhibitory Peptides Using an Enzymatic Membrane Reactor
Continuous processing in the production of peptides is an area of increased interest. In this study, an enzymatic membrane reactor (EMR) was developed whereby whey protein isolate was used as a substrate to prepare DPP-IV inhibitory and radical scavenging peptides via enzymatic hydrolysis. Two separate enzymes were tested: Corolase 2TS and Protamex in conventional batch processes and the EMR. Neither enzyme was considered effective at producing peptides with radical scavenging activity when measured using a DPPH assay. However, both enzymes were capable of producing DPP-IV inhibitory peptides. Corolase and Protamex both produced similar DPP-IV inhibition levels upon completion of batch experiments. In the EMR process, permeate in the Protamex run showed 33.7% lower IC50 value compared to the continuous Corolase run. Protamex was a better enzyme at producing the DPP-IV inhibitory effect. The continuous (EMR) production method showed an increased productivity over batch for both enzymes.
KeywordsEnzymatic membrane reactor DPP-IV inhibitory peptides Peptides with specific bioactivity Whey protein
Enterprise Ireland is acknowledged for financial support of this research.
- Aart, V., Catharina M., Zeeland-Wolbers V., Maria L., Gilst V., Hendrikus W., Nelissen B. and Maria J. (2009). "Egg protein hydrolysates." Patent, WO 128713: 2009.Google Scholar
- Adler-Nissen, J. (1986). Enzymic hydrolysis of food proteins. New York: Elsevier Applied Science Publishers.Google Scholar
- Ha, G. E., Chang, O. K., Jo, S. M., Han, G. S., Park, B. Y., Ham, J. S., & Jeong, S. G. (2015). Identification of antihypertensive peptides derived from low molecular weight casein hydrolysates generated during fermentation by Bifidobacterium longum KACC 91563. Korean Journal for Food Science of Animal Resources, 35(6), 738–747.CrossRefGoogle Scholar
- ISO F. (2009). Feed products–general guidelines for the determination of nitrogen by the Kjeldahl method. International Organization for Standardiza-tion, Geneva, Switzerland, 1871, 2009.Google Scholar
- Jakovetić, S., Luković, N., Jugović, B., Gvozdenović, M., Grbavčić, S., Jovanović, J., & Knežević-Jugović, Z. (2015). Production of antioxidant egg white hydrolysates in a continuous stirred tank enzyme reactor coupled with membrane separation unit. Food and Bioprocess Technology, 8(2), 287–300.CrossRefGoogle Scholar
- Janson, J.-C. (2012). Protein purification: Principles, high resolution methods, and applications, John Wiley & Sons.Google Scholar
- Jemil, I., Jridi, M., Nasri, R., Ktari, N., Salem, R. B. S.-B., Mehiri, M., Hajji, M., & Nasri, M. (2014). Functional, antioxidant and antibacterial properties of protein hydrolysates prepared from fish meat fermented by Bacillus subtilis A26. Process Biochemistry, 49(6), 963–972.CrossRefGoogle Scholar
- Noble, R. D. and S. A. Stern (1995). Membrane separations technology: Principles and applications, Elsevier.Google Scholar
- Seader, J. D. and E. J. Henley (2011). "Separation process principles." John Wiley and Sons, Inc.Google Scholar