Current Osteoporosis Reports

, Volume 17, Issue 4, pp 178–185 | Cite as

RNA-seq in Skeletal Biology

  • Ugur AyturkEmail author
Skeletal Biology and Regulation (M Forwood and A Robling, Section Editors)
Part of the following topical collections:
  1. Topical Collection on Skeletal Biology and Regulation


Purpose of Review

The goal of this paper is to review state-of-the-art transcriptome profiling methods and their recent applications in the field of skeletal biology.

Recent Findings

Next-generation sequencing of mRNA (RNA-seq) methods have been established and routinely used in skeletal biology research. RNA-seq has led to the identification of novel genes and transcription factors involved in skeletal development and disease, through its application in small and large animal models, as well as human tissue and cells. With the availability of advanced techniques such as single-cell RNA-seq, novel cell types in skeletal tissues are being identified.


As the sequencing technologies are rapidly evolving, the exciting discoveries supported by transcriptomics will continue to emerge and improve our understanding of the biology of the skeleton.


RNA-seq Single-cell RNA-seq Bone Cartilage Ligament Wnt signaling 


Compliance with Ethical Standards

Conflict of Interest

Ugur Ayturk declares no conflict of interest.

Human and Animal Rights and Informed Consent

This article does not contain any studies with human or animal subjects performed by any of the authors.


Papers of particular interest, published recently, have been highlighted as: • Of importance •• Of major importance

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Musculoskeletal Integrity ProgramHospital for Special SurgeryNew YorkUSA

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