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Wuhan University Journal of Natural Sciences

, Volume 24, Issue 5, pp 409–416 | Cite as

Pyrazole-Triazine Conjugate as Highly Selective and Sensitive Fluorescence Probe for Silver (I) Detection and Its Imaging in Living Cells

  • Yanhong Liu
  • Bingqiong Yu
  • Qingya Zhu
  • Kun YanEmail author
Chemistry and Physics
  • 8 Downloads

Abstract

A new fluorescence probe 2,2′-(6-(4-(diethylamino) phenyl)-1,3,5-triazine-2,4-diyl)bis(1H-pyrazole-1,3-diyl) diacetate (EATPA) based on 1, 3, 5-triazine was designed and synthesized. It exhibited distinct fluorescence quenching in the presence of silver ions that can be used for highly sensitive and selective detection of Ag+. Fluorescence titration and Job’s plot analysis revealed the formation of [Ag(EATPA)2]+ entity with high binding constant (1.43×104 L/mol) and low detection limit (0.882 µmol/L). Furthermore, live-cell imaging experiments demonstrated that EATPA is potentially applicable for the tracking of Ag+ in living cells.

Key words

fluorescence probe silver ions fluorescence quenching detection limit cell imaging 

CLC number

O 61 

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Copyright information

© Wuhan University and Springer-Verlag GmbH Germany 2019

Authors and Affiliations

  • Yanhong Liu
    • 1
  • Bingqiong Yu
    • 1
  • Qingya Zhu
    • 1
  • Kun Yan
    • 1
    Email author
  1. 1.College of Chemistry and Molecular SciencesWuhan UniversityWuhan, HubeiChina

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