Wuhan University Journal of Natural Sciences

, Volume 24, Issue 1, pp 57–63 | Cite as

Accumulation and Elimination Kinetics of 2-Methylisoborneol in Crucian Carp (Carassius carassius)

  • Meng Yang
  • Jiaming Hu
  • Liming LiuEmail author
  • Xiaotao Shi
  • Guoyong Liu
  • Yanxia Zuo


2-methylisoborneol (2-MIB) is a common, earthy-smelling compound that causes taste and odor issues in aquaculture. To quantify the distribution, absorption, and depuration of 2-MIB in crucian carp (Carassius carassius), we detected and measured 2-MIB it via microwave, headspace solid-phase microextraction (HS-SPME), and gas chromatography before and after exposing fish to two different concentrations of 2-MIB (200 ng/L and 500 ng/L). The results showed that during the exposure time, the fish absorbed 2-MIB rapidly within 4 h. After exposure to 2-MIB for 120 h, the 2-MIB in gill and liver were rapidly removed. The processes of accumulation and elimination of 2-MIB in fish were investigated using the one-compartment kinetic model. The modeling results showed that the range of the uptake rate constant (k1) was 0.49-6.91. The range of the elimination rate constant (k2) was 0.05-0.27 and uptake rates were higher than elimination rates. The bioconcentration factor (BCF) was estimated according to the physicochemical properties of 2-MIB, and the bioaccumulation of 2-MIB in fish had a close correlation with the octanol/water partition coefficient (Kow).

Key words

earthy-musty odor bioaccumulation elimination 2-methylisoborneol (2-MIB) fish 

CLC number

S 949 


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  1. [1]
    Lovell R T. New off-flavors in pond-cultured channel catfish [J]. Aquaculture, 1983, 30 (1-4): 329–334.CrossRefGoogle Scholar
  2. [2]
    Nakajima M, Ogura T, Kusama Y, et al. Inhibitory effects of odor substances, geosmin and 2-methylisoborneol, on early development of sea urchins [J]. Water Research, 1996, 30(10): 2508–2511.CrossRefGoogle Scholar
  3. [3]
    Gerber N, Lechevalier H. Geosmin, an earthly-smelling substance isolated from actinomycetes [J]. Applied and Environmental Microbiology, 1965, 13(6): 935–938.Google Scholar
  4. [4]
    Robertson R F, Hammond A, Jauncey K, et al. An investigation into the occurrence of geosmin responsible for earthyemusty taints in UK farmed rainbow trout, Onchorhynchus mykiss [J]. Aquaculture, 2006, 259(1): 153–163.CrossRefGoogle Scholar
  5. [5]
    Burr G S, Wolters W R, Schrader K K, et al. Impact of depuration of earthy-musty off-flavors on fillet quality of Atlantic salmon, Salmo salar, cultured in a recirculating aquaculture system [J]. Aquacultural Engineering, 2012, 50(1): 28–36.CrossRefGoogle Scholar
  6. [6]
    From J, Hørlyck V. Sites of uptake of geosmin, a cause of earthy-flavor, in rainbow trout [J]. Canadian Journal of Fisheries and Aquatic Sciences, 1984, 41(8): 1224–1226.CrossRefGoogle Scholar
  7. [7]
    Robertson R F, Jauncey K, Beveridge M C M, et al. Depuration rates and the sensory threshold concentration of geosmin responsible for earthy-musty taint in rainbow trout, Onchorhynchus mykiss [J]. Aquaculture, 2005, 245(1): 89–99.CrossRefGoogle Scholar
  8. [8]
    Barber M C. A review and comparison of models for predicting dynamic chemical bioconcentration in fish [J]. Environmental Toxicology and Chemistry, 2003, 22(9): 1963–1992.CrossRefGoogle Scholar
  9. [9]
    Howgate P. Tainting of farmed fish by geosmin and 2-methyl-iso-borneol: A review of sensory aspects and of uptake/ depuration [J]. Aquaculture, 2004, 234(1-4): 155–181.CrossRefGoogle Scholar
  10. [10]
    Wang M Y, Yu W, Geyer H J. Two-compartment thermodynamic model for bioconcentration of hydrophobic organic chemicals by alga: Quantitative relationship between bioconcentration factor and surface area of marine algae or octanol/ water partition coefficient [J]. Chemosphere, 1997, 35(8): 1781–1797.CrossRefGoogle Scholar
  11. [11]
    Bertelsen S L, Hoffman A D, Gallinat C A, et al. Evaluation of lgK ow, and tissue lipid content as predictors of chemical partitioning to fish tissues [J]. Environmental Toxicology and Chemistry, 1998, 17(8): 1447–1455.Google Scholar
  12. [12]
    Yuan X C, Zhao W W. China Fishery Statistical Yearbook [M]. Beijing: China Statistics Press, 2016(Ch).Google Scholar
  13. [13]
    Yin S R, Xu L P. A preliminary study on the relationship between freshwater algae and the off-flavor in fishes [J]. Journal of Dalian Fisheries University, 2003, 18(2): 156–157(Ch).Google Scholar
  14. [14]
    Liu L, Li L, Zuo Y, et al. Adsorption of 2-methylisoborneol and geosmin by a low-cost hybrid adsorbent synthesized from fly ash and bentonite [J]. Journal of Water Supply: Research and Technology-AQUA, 2011, 60(8): 478–485.CrossRefGoogle Scholar
  15. [15]
    Li L, Wan N, Gan N Q, et al. Annual dynamics and origins of the odorous compounds in the pilot experimental area of Lake Dianchi, China [J]. Water Science and Technology, 2007, 55(5): 43–50.CrossRefGoogle Scholar
  16. [16]
    Xu Y, Li W, Wu W Z, et al. Study on aquatic off-flavors in eutrophic Donghu Lake [J]. Acta Ecologica Sinica, 1999, 19 (2): 212–216(Ch).Google Scholar
  17. [17]
    Liu Q. Study on the Use of Potassium Permanganate in Combination with Damp Powered Activated Carbon for the Removal of Odor [D]. Shaanxi: Xi’an University of Architecture and Technology, 2004(Ch).Google Scholar
  18. [18]
    Yagi O, Sugiura N, Sudo R. Chemical and biological factors on the musty odor occurrence in Lake Kasumigaura [J]. Japanese Journal of Limnology, 2009, 46 (1): 32–40.CrossRefGoogle Scholar
  19. [19]
    Wang F, Lin S, Chen Y. Study on the existence, distribution and bioaccumulation of chlorophenols in crucian carps [J]. Journal of Hygiene Research, 1999, 28(3): 169–171.Google Scholar
  20. [20]
    Martin J F, Bennett L W, Graham W H. Off-flavor in the channel catfish (Ictalurus punctatus) due to 2-methylisoborneol and its dehydration products [J]. Iwa Publishing, 1988, 20(8-9): 99–105.Google Scholar
  21. [21]
    Qiao F, Li H, Li D, et al. Comparative analysis of fatty acid profiles in different tissues of five economic fish species in winter and summer [J]. Journal of Fisheries of China, 2018, 42(1): 80–88(Ch).Google Scholar
  22. [22]
    Johnsen P B, Lloyd S W. Influence of fat content on uptake and depuration of the off-flavor 2-methylisoborneol by channel catfish (Ictalurus punctatus) [J]. Canadian Journal of Fisheries and Aquatic Sciences, 1992, 49 (11): 2406–2411.CrossRefGoogle Scholar
  23. [23]
    Pettit S L, Rodriguez-Gonzalez L, Michaels J T, et al. Parameters influencing the photocatalytic degradation of geosmin and 2-methylisoborneol utilizing immobilized TiO2 [J]. Catalysis Letters, 2014, 144(8): 1460–1465.CrossRefGoogle Scholar
  24. [24]
    Li L, Jiang M, Wang Y, et al. Accumulation kinetics of water accommodated fraction in No.0 fuel oil and Pinghu crude oil in Sparus macrocephlus [J]. Chinese Journal of Applied & Environmental Biology, 2014, 20(2): 286–290(Ch).Google Scholar
  25. [25]
    Yang Y P, Xiong G Q, Cheng W. Review on formation mechanism, analysis and removal technologies of fishy off-odor [J]. Food Science, 2009, 30(23): 533–538.Google Scholar
  26. [26]
    Bintein S, Devillers J, Karcher W. Nonlinear dependence of fish bioconcentration on n-octanol/water partition coefficient [J]. Sar and Qsar in Environmental Research, 1993, 1(1): 29–39.CrossRefGoogle Scholar
  27. [27]
    Kenaga E E, Goring C A. Relationship between water solubility, soil sorption, octanol-water partitioning, and concentration of chemicals in biota [C]// Fourth International Congress of Pesticide Chemistry. New York: Academic Press, 1979: V–33.Google Scholar
  28. [28]
    Song W, O’Shea K E. Ultrasonically induced degradation of 2-methylisoborneol and geosmin [J]. Water Research, 2007, 41(12): 2672–2678.CrossRefGoogle Scholar
  29. [29]
    Forrester P N, Prinyawiwatkul W, Godber J S, et al. Treatment of catfish fillets with citric acid causes reduction of 2-methylisoborneol, but not musty flavor [J]. Journal of Food Science, 2002, 67(7): 2615–2618.CrossRefGoogle Scholar
  30. [30]
    Mirelesdewitt C, Kleinholz C W, Ms D G V, et al. Evaluation of acid and alkaline processing to remove muddy off-flavors in channel catfish (Ictalurus punctatus) [J]. Journal of Aquatic Food Product Technology, 2007, 16(2): 77–90.CrossRefGoogle Scholar
  31. [31]
    Tucker C S. Off-flavor problems in aquaculture [J]. Reviews in Fisheries Science, 2000, 8(1): 45–88.CrossRefGoogle Scholar
  32. [32]
    Sklenar K S, Horne A J. Horizontal distribution of geosmin in a reservoir before and after copper treatment [J]. Water Science and Technology, 1999, 40(6): 229–237.CrossRefGoogle Scholar
  33. [33]
    Murray-Gulde C L, Heatley J E, Schwartzman A L, et al. Algicidal effectiveness of clearigate, cutrine-plus, and copper sulfate and margins of safety associated with their use [J]. Archives of Environmental Contamination and Toxicology, 2002, 43(1): 19–27.CrossRefGoogle Scholar
  34. [34]
    Lü H, Hu W, Xiong S, et al. Depuration and starvation improves flesh quality of grass carp (Ctenopharyngodon idella) [J]. Aquaculture Research, 2018, 49 (4): 3196–3206.CrossRefGoogle Scholar
  35. [35]
    Palmeri G, Turchini G M, Caprino F, et al. Biometric, nutritional and sensory changes in intensively farmed Murray cod (Maccullochella peelii peelii, Mitchell) following different purging times [J]. Food Chemistry, 2008, 107 (4): 1605–1615.CrossRefGoogle Scholar
  36. [36]
    Persson P E. Sensory properties and analysis of two muddy odour compounds, geosmin and 2-methylisoborneol, in water and fish [J]. Water Research, 1980, 14(8): 1113–1118.CrossRefGoogle Scholar
  37. [37]
    Yamprayoon J, Noomhorm A. Effects of preservation methods on geosmin content and off-flavor in Nile tilapia (Oreochomis niloticus) [J]. Journal of Aquatic Food Product Technology, 2000, 9(4): 95–107.CrossRefGoogle Scholar

Copyright information

© Wuhan University and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Meng Yang
    • 1
  • Jiaming Hu
    • 1
  • Liming Liu
    • 1
    Email author
  • Xiaotao Shi
    • 1
  • Guoyong Liu
    • 1
  • Yanxia Zuo
    • 2
  1. 1.Hubei International Scientific and Technological Cooperation Center of Ecological Conservation and Management in Three Gorges AreaHubeiChina
  2. 2.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiologyChinese Academy of SciencesHubeiChina

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