Wuhan University Journal of Natural Sciences

, Volume 24, Issue 1, pp 30–36 | Cite as

An Automatic Solid Phase Extraction and Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry for Determination of Seven Microcystins at Ultra-Trace Levels in Surface Water

  • Fei ShenEmail author
  • Yanjuan Xu
  • Ye Wang
  • Jing Chen


A method was developed for the detection of seven microcystins (microcystin-LR, RR, YR, LA, LY, LW and LF) in surface water using automatic solid-phase extraction (A-SPE) coupled with ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The automated solid-phase extraction system was used to extract microcystins (MCs) from water samples. UPLC-MS/MS was used to determine MCs concentrations in just 5 min. Method detection limits were from 0.3 to 0.9 ng/L, microcystin recoveries ranged from 83.8% to 114%, and the relative standard deviation (RSD) varied from 5.6% to 12.5%. This analytical approach was found to be simple, highly sensitive, accurate, which required little manual operation. Additionally, to validate this analytical method, A-SPE+UPLC-MS/MS was applied to characterize the concentration of MCs in Taihu Lake, Wuxi, China.

Key words

microcystins automatic solid-phase extraction ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) 

CLC number

O 656.3 


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Copyright information

© Wuhan University and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Environmental and Civil EngineeringJiangnan UniversityJiangsuChina
  2. 2.Wuxi Environmental Monitoring Central StationJiangsuChina

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