The role of increased synovial fluid A disintegrin and metalloproteinase with thrombospondin motifs4 and serglycin levels in osteoarthritis
- 20 Downloads
The first research to determine synovial fluid ADAMTS4 and serglycin levels in osteoarthritis and OA progression.
We aimed to determine ADAMTS4 and serglycin levels, interactions, and changes in the synovial fluid of knee OA, and also to determine effective in OA progression.
A case-control study was carried out including a total of 88 participants (29 patients late OA [LOA], 28 early OA [EOA], and 30 controls). Synovial fluid serglycin and ADAMTS4 levels were measured by commercially available ELISA kits, and knee functions of the patients were evaluated with The Western Ontario and McMaster Universities Osteoarthritis score (WOMAC). Logistic regression analysis was applied for the associated with progression of OA.
Synovial fluid ADAMTS4 and serglycin levels were significantly higher in LOA than EOA and control groups (p < .001 and p < .001; p = .038 and p = .007, respectively). All parameters were evaluated after adjustment for age. LOA patients had significantly higher levels of WOMAC score than EOA and controls (p < .001 and p < .001). According to the logistic regression analysis, synovial fluid ADAMTS4, serglycin levels, and WOMAC score were found to be significantly associated with progression of OA.
KeywordsADAMTS4 Knee osteoarthritis Serglycin Synovial fluid WOMAC score
We would like to thank patients and staff who participated in the study.
The author did not receive financial support from any institution for this study.
Compliance with ethical standards
Conflict of interest
The author declares no conflict of interest.
- 11.Kellgren JH, Lawrence JS (1957) Radiological assessment of osteoarthrosis. Ann Rheum Dis 16:494e502Google Scholar
- 12.Bellamy N, Buchanan WW, Goldsmith CH, Campbell J, Stitt LW (1988) Validation study of WOMAC: a health status instrument for measuring clinically important patient relevant outcomes to anti-rheumatic drug therapy in patients with osteoarthritis of the hip or knee. J Rheumatol 15:1833–1840Google Scholar
- 14.Miller RG. (1966) Simultaneous statistical inference. McGraw-Hill: New YorkGoogle Scholar
- 16.He Y, Zheng Q, Jiang M, Sun S, Christiansen TG, Kassem M, Karsdal MA, Bay-Jensen AC (2015) The effect of protease inhibitors on the induction of osteoarthritis-related biomarkers in bovine full-depth cartilage explants. PLoS One 10(4):e0122700. https://doi.org/10.1371/journal.pone.0122700 CrossRefPubMedPubMedCentralGoogle Scholar
- 18.Roberts S, Evans H, Wright K, van Niekerk L, Caterson B, Richardson JB, Kumar KHS, Kuiper JH (2015) ADAMTS-4 activity in synovial fluid as a biomarker of inflammation and effusion. Osteoarthr Cartil 23(9):1622–1626. https://doi.org/10.1016/j.joca.2015.05.006 CrossRefPubMedPubMedCentralGoogle Scholar
- 19.Peng S, Zheng Q, Zhang X, Dai L, Zhu J, Pi Y, Hu X, Cheng W, Zhou C, Sha Y, Ao Y (2013) Detection of ADAMTS-4 activity using a fluorogenic peptide-conjugated Au nanoparticle probe in human knee synovial fluid. ACS Appl Mater Interfaces 5(13):6089–6096. https://doi.org/10.1021/am400854z. CrossRefPubMedGoogle Scholar
- 26.Kataoka Y, Ariyoshi W, Okinaga T, Kaneuji T, Mitsugi S, Takahashi T, Nishihara T (2013) Mechanisms involved in suppression of ADAMTS4 expression in synoviocytes by high molecular weight hyaluronic acid. Biochem Biophys Res Commun 432(4):580–585. https://doi.org/10.1016/j.bbrc.2013.02.043 CrossRefPubMedGoogle Scholar
- 27.Erwin WM, Islam D, Inman RD, Fehlings MG, Tsui FWL (2011) Notochordal cells protect nucleus pulposus cells from degradation and apoptosis: implications for the mechanisms of intervertebral disc degeneration. Arthritis Res Ther 13(6):R215. https://doi.org/10.1186/ar3548 CrossRefPubMedPubMedCentralGoogle Scholar