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Arthropod-Plant Interactions

, Volume 13, Issue 6, pp 915–921 | Cite as

Concentrations of sunflower phenolics appear insufficient to explain resistance to floret- and seed-feeding caterpillars

  • Jarrad R. PrasifkaEmail author
  • Christopher M. Wallis
Original Paper
  • 47 Downloads

Abstract

Cultivated sunflowers, Helianthus annuus L., show significant variation in susceptibility to insect pests, though specific mechanisms of resistance are not clear. Plant secondary compounds, including phenolics, are often associated with resistance to insects and pathogens. Because several phenolics are present in sunflower florets, wild sunflowers and cultivated inbred lines were sampled to document natural variation in free phenolics and evaluate their potential effects on floret-feeding pests. Four di-O-caffeoylquinic acid isomers were the largest contributors to total phenolic content of disc florets. When identified phenolics were combined with unidentified flavonoid glycosides, total phenolic concentration in inbred lines varied fourfold (≈ 18–72 mg/g), and was significantly greater than that in wild sunflowers. Two lab assays with 50 or 100 mg/g chlorogenic acid showed inconsistent reductions in mass or increases in mortality of sunflower moth (Homoeosoma electellum Hulst) larvae after 9 days, and tests using floret tissue with low or high levels of total phenolics did not show an effect of tissue type on H. electellum development. Tests for correlation between total phenolics and field data on susceptibility of sunflowers to another floret-feeding pest, banded sunflower moth (Cochylis hospes Walsingham), did not show any significant association. Cumulatively, results suggest there may be minor effects of phenolic compounds on floret-feeding insects, but these alone are insufficient for plant defense. However, variation in concentration of phenolic compounds may remain valuable, both as a component of plant defense and a source of oxidative stability in sunflower oil.

Keywords

Sunflower Chlorogenic acid Host plant resistance Di-O-caffeoylquinic acid Sunflower moth 

Notes

Acknowledgements

We appreciate help from Tandi Thompson in sample preparation and Jamie Miller-Dunbar in collecting data from tests of larval development.

Supplementary material

11829_2019_9706_MOESM1_ESM.pdf (73 kb)
Supplementary material 1 (PDF 72 kb)
11829_2019_9706_MOESM2_ESM.pdf (85 kb)
Supplementary material 2 (PDF 85 kb)

References

  1. Beregovoy VH, Hein GL, Hammond RB (1989) Variations in flight phenology and new data on the distribution of the banded sunflower moth (Lepidoptera: Cochylidae). Environ Entomol 18:273–277CrossRefGoogle Scholar
  2. Brewer GJ, Anderson MD (1990) Modification of the effect of Bacillus thuringiensis on sunflower moth (Lepidoptera: Pyralidae) by dietary phenols. J Econ Entomol 83:2219–2224CrossRefGoogle Scholar
  3. Charlet LD, Aiken RM, Seiler GJ, Chirumamilla A, Hulke BS, Knodel JJ (2008) Resistance in cultivated sunflower to the sunflower moth (Lepidoptera: Pyralidae). J Agric Urban Entomol 25:245–257CrossRefGoogle Scholar
  4. Charlet LD, Seiler GJ, Miller JF, Hulke BS, Knodel JJ (2009) Resistance among cultivated sunflower germplasm to the banded sunflower moth (Lepidoptera: Tortricidae) in the Northern Great Plains. Helia 32:1–9CrossRefGoogle Scholar
  5. Charlet LD, Seiler GJ, Grady KA, Hulke BS, Chirumamilla A (2010) Resistance in cultivated sunflower germplasm to the red sunflower seed weevil (Coleoptera: Curculionidae) in the Northern Great Plains. J Kansas Entomol Soc 83:51–57CrossRefGoogle Scholar
  6. Cheynier V, Comte G, Davies KM, Lattanzio V, Martens S (2013) Plant phenolics: recent advances on their biosynthesis, genetics, and ecophysiology. Plant Physiol Biochem 72:1–20CrossRefGoogle Scholar
  7. Chirumamilla A, Knodel JJ, Charlet LD, Hulke BS, Foster SP, Ode PJ (2014) Ovipositional preference and larval performance of the banded sunflower moth (Lepidoptera: Tortricidae) and its larval parasitoids on resistant and susceptible lines of sunflower (Asterales: Asteraceae). Environ Entomol 43:58–68CrossRefGoogle Scholar
  8. De Leonardis A, Macciola V, Di Rocci A (2003) Oxidative stability of cold-pressed sunflower oil using phenolic compounds of the same seeds. J Sci Food Agric 83:523–528CrossRefGoogle Scholar
  9. FAO [Food and Agriculture Organization of the United Nations] (2017) FAO Food Outlook: Oilseeds. FAO, Rome, Italy. http://www.fao.org/fileadmin/templates/est/COMM_MARKETS_MONITORING/Oilcrops/Documents/Food_outlook_oilseeds/FO_June_2017.pdf. Accessed 1 Dec 2018
  10. Howe GA, Jander G (2008) Plant immunity to insect herbivores. Annu Rev Plant Biol 59:41–66CrossRefGoogle Scholar
  11. Ikonen A, Tahvanainen J, Roininen H (2001) Chlorogenic acid as an antiherbivore defence of willows against leaf beetles. Entomol Exp Appl 99:47–54CrossRefGoogle Scholar
  12. Karban R, Agrawal AA (2002) Herbivore offense. Annu Rev Ecol Syst 33:641–664CrossRefGoogle Scholar
  13. Leiss KA, Maltese F, Choi YH, Verpoorte R, Klinkhamer PGL (2009) Identification of chlorogenic acid as a resistance factor for thrips in chrysanthemum. Plant Physiol 150:1567–1575CrossRefGoogle Scholar
  14. Liang Q, Cui J, Li H, Liu J, Zhao G (2013) Florets of sunflower (Helianthus annuus L.): potential new sources of dietary fiber and phenolic acids. J Agric Food Chem 61:3435–3442CrossRefGoogle Scholar
  15. Michaud JP, Grant AK (2009) The nature of resistance to Dectes texanus (Col., Cerambycidae) in wild sunflower, Helianthus annuus. J Appl Entomol 133:518–523CrossRefGoogle Scholar
  16. Mikulic-Petkovsek M, Schmitzer V, Slatnar A, Stampar F, Veberic R (2012) Composition of sugars, organic acids, and total phenolics in 25 wild or cultivated berry species. J Food Sci 77:C1064–C1070CrossRefGoogle Scholar
  17. Mullin CA, Alfatafta AA, Harman JL, Serino AA, Everett SL (1991) Corn rootworm feeding on sunflower and other Compositae: influence of floral terpenoid and phenolic factors. In: Naturally occurring pest bioregulators. ACS Symposium series, pp 278–292Google Scholar
  18. Ossipov V, Haukioja E, Ossipova S, Hanhimaki S, Pihlaja K (2001) Phenolic and phenolic-related factors as determinants of suitability of mountain birch leaves to an herbivorous insect. Biochem Syst Ecol 29:223–240CrossRefGoogle Scholar
  19. Prasifka JR (2015) Variation in the number of capitate glandular trichomes in wild and cultivated sunflower germplasm and its potential for use in host plant resistance. Plant Genet Resour 13:68–74CrossRefGoogle Scholar
  20. Prasifka JR, Hulke BS (2016) Relative susceptibility of sunflower maintainer lines and resistance sources to natural infestations of the banded sunflower moth (Lepidoptera: Tortricidae). Can Entomol 148:736–741CrossRefGoogle Scholar
  21. Prasifka JR, Hulke BS, Seiler GJ (2014) Pericarp strength of sunflower and its value for plant defense against the sunflower moth, Homoeosoma electellum. Arthropod Plant Interact 8:101–107CrossRefGoogle Scholar
  22. Prasifka JR, Spring O, Conrad J, Cook LW, Palmquist DE, Foley ME (2015) Sesquiterpene lactone composition of wild and cultivated sunflowers and biological activity against an insect pest. J Agric Food Chem 63:4042–4049CrossRefGoogle Scholar
  23. Rogers CE, Thompson TE (1978) Resistance of wild Helianthus species to an aphid, Masonaphis masoni. J Econ Entomol 71:221–222CrossRefGoogle Scholar
  24. Rogers CE, Westbrook KE (1985) Sunflower moth (Lepidoptera: Pyralidae): overwintering and dynamics of spring emergence in the southern Great Plains. Environ Entomol 14:607–611CrossRefGoogle Scholar
  25. SAS Institute Inc (2016) Base SAS 9.4 procedures guide: statistical procedures, 5th edn. SAS Institute Inc., CaryGoogle Scholar
  26. Schneiter AA, Miller JF (1981) Description of sunflower growth stages. Crop Sci 21:901–903CrossRefGoogle Scholar
  27. Teetes GL, Randolph NM (1969) Some new host plants of the sunflower moth in Texas. J Econ Entomol 62:264–265CrossRefGoogle Scholar
  28. USDA [United States Department of Agriculture] (2017) Germplasm Resources Information Network—(GRIN). https://npgsweb.ars-grin.gov/gringlobal/search.aspx. Accessed 1 Dec 2018
  29. Wallis CM, Chen J (2012) Grapevine phenolic compounds in xylem sap and tissues are significantly altered during infection by Xylella fastidiosa. Phytopathol 102:816–826CrossRefGoogle Scholar
  30. Wallis C, Eyles A, Chorbadjian R, McSpadden-Gardner B, Hansen R, Cipollini D, Herms D, Bonello P (2008) Systemic induction of phloem secondary metabolism and its relationship to resistance to a canker pathogen in Austrian pine. New Phytol 177:767–778CrossRefGoogle Scholar
  31. Wilson RL (1990) Rearing the sunflower moth (Lepidoptera: Pyralidae) for use in field evaluation of sunflower germplasm. J Kans Entomol Soc 63:208–210Google Scholar

Copyright information

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2019

Authors and Affiliations

  1. 1.USDA-ARS, Edward T. Schafer Agricultural Research CenterFargoUSA
  2. 2.Crop Diseases, Pests and Genetics Research UnitUSDA-ARS, San Joaquin Valley Agricultural Sciences CenterParlierUSA

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