Differential physiological and molecular responses of three-leaf stage barley (Hordeum vulgare L.) under salt stress within hours

  • Cüneyt UçarlıEmail author
  • Filiz Gürel
Original Article


Salt stress is first perceived by the plant roots and inhibits plant growth in the short-term by inducing osmotic stress caused by decreased water availability. In this study, 160 mM NaCl was applied to three-leaf-stage barley plants (Hordeum vulgare L. cv. Martı) for a short period (0, 2, and 26 h) Osmolyte accumulation and ion leakage was significantly increased after salt stress treatment compared with control conditons in both leaf and root tissues within 2 h. We have also found that expressions of transcription factors HvDRF2 and HvWRKY12, associated with abiotic stress including salinity and drought stress, were quite low in root and shoots in control conditions; however, salt stress resulted into upregulation of HvDRF2 expression as 28.8- and 26.6-fold in roots and leaves, respectively, within 26 h. While salt stress-induced significantly upregulation of HvPR1A (26.4-fold) HvNHX1 (sevenfold) in 2 h at P < 0.05 level, significant upregulation of HvMT2 (8.2-fold) and HvDHN3 (4.7-fold) was observed at 26 h after salt treatment in roots. In leaves, HvMT2 (12.7-fold), HvNHX1 (12.1-fold) and HvBAS1 (3.4-fold) were significantly upregulated under salt stress. Only HvLHCB mRNA level was significantly decreased as 2- and 5.6-fold in leaf tissues with salinityin 2 and 26 h, respectively.


Salt stress Hordeum vulgare Osmolality HvBAS1 HvMT2 HvDRF2 HvPR1A 



This work was supported by the Scientific Research Projects Coordination Unit of Istanbul University, project no. 23966.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Korean Society for Plant Biotechnology 2019

Authors and Affiliations

  1. 1.Department of Molecular Biology and GeneticsIstanbul UniversityIstanbulTurkey

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