Advertisement

Differential physiological and molecular responses of three-leaf stage barley (Hordeum vulgare L.) under salt stress within hours

  • Cüneyt UçarlıEmail author
  • Filiz Gürel
Original Article
  • 32 Downloads

Abstract

Salt stress is first perceived by the plant roots and inhibits plant growth in the short-term by inducing osmotic stress caused by decreased water availability. In this study, 160 mM NaCl was applied to three-leaf-stage barley plants (Hordeum vulgare L. cv. Martı) for a short period (0, 2, and 26 h) Osmolyte accumulation and ion leakage was significantly increased after salt stress treatment compared with control conditons in both leaf and root tissues within 2 h. We have also found that expressions of transcription factors HvDRF2 and HvWRKY12, associated with abiotic stress including salinity and drought stress, were quite low in root and shoots in control conditions; however, salt stress resulted into upregulation of HvDRF2 expression as 28.8- and 26.6-fold in roots and leaves, respectively, within 26 h. While salt stress-induced significantly upregulation of HvPR1A (26.4-fold) HvNHX1 (sevenfold) in 2 h at P < 0.05 level, significant upregulation of HvMT2 (8.2-fold) and HvDHN3 (4.7-fold) was observed at 26 h after salt treatment in roots. In leaves, HvMT2 (12.7-fold), HvNHX1 (12.1-fold) and HvBAS1 (3.4-fold) were significantly upregulated under salt stress. Only HvLHCB mRNA level was significantly decreased as 2- and 5.6-fold in leaf tissues with salinityin 2 and 26 h, respectively.

Keywords

Salt stress Hordeum vulgare Osmolality HvBAS1 HvMT2 HvDRF2 HvPR1A 

Notes

Acknowledgements

This work was supported by the Scientific Research Projects Coordination Unit of Istanbul University, project no. 23966.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

References

  1. Agarwal PK, Shukla PS, Gupta K, Jha B (2013) Bioengineering for salinity tolerance in plants: state of the art. Mol Biotechnol 54:102–123PubMedCrossRefGoogle Scholar
  2. Ali S, Mir ZA, Tyagi A, Bhat JA, Chandrashekar N, Papolu PK, Rawat S, Grover A (2017) Identification and comparative analysis of Brassica juncea pathogenesis-related genes in response to hormonal, biotic and abiotic stresses. Acta Physiol Plant 39:268CrossRefGoogle Scholar
  3. Amitai-Zeigerson H, Scolnik PA, Bar-Zvi D (1995) Tomato Asr1mRNA and protein are transiently expressed following salt stress, osmotic stress and treatment with abscisic acid. Plant Sci 110:205–213CrossRefGoogle Scholar
  4. Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58CrossRefGoogle Scholar
  5. Battaglia M, Olvera-Carrillo Y, Garciarrubio A, Campos F, Covarrubias A (2008) The enigmatic LEA proteins and other hydrophilins. Plant Physiol 148:6–24PubMedPubMedCentralCrossRefGoogle Scholar
  6. Blake T, Blake V, Bowman J, Abdel-Haleem H (2011) Barley feed uses and quality improvement. In: Ullrich SE (ed) Barley: production improvement and uses. Wiley-Blackwell, Oxford, pp 522–531Google Scholar
  7. Deinlein U, Stephan AB, Horie T, Luo W, Xu G, Schroeder JI (2014) Plant salt-tolerance mechanisms. Trends Plant Sci 19:371–379PubMedPubMedCentralCrossRefGoogle Scholar
  8. Dietz KJ, Jacob S, Oelze ML, Laxa M, Tognetti V, de Miranda SMN, Baier M, Finkemeier I (2006) The function of peroxiredoxins in plant organelle redox metabolism. J Exp Biol 57:1697–1709Google Scholar
  9. Dominguez PG, Carrari F (2015) ASR1 transcription factor and its role in metabolism. Plant Signal Behav 10:e992751PubMedPubMedCentralCrossRefGoogle Scholar
  10. El-Tayeb MA (2005) Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regul 45:215–224CrossRefGoogle Scholar
  11. FAO (2017) FAOSTAT statistical database. https://www.fao.org/faostat/en/#data/QC. Accessed 17 Dec 2018
  12. FAO (2018) The state of food security and nutrition in the world: building climate resilience for food security and nutrition. https://www.fao.org/3/I9553EN/i9553en.pdf. Accessed 20 Dec 2018
  13. Fricke W, Akhiyarova G, Wei W, Alexandersson E, Miller A, Kjellbom PO et al (2006) The short-term growth response to salt of the developing barley leaf. J Exp Bot 57:1079–1095PubMedCrossRefGoogle Scholar
  14. Fukuda A, Chiba K, Maeda M, Nakamura A, Maeshima M, Tanaka Y (2004) Effect of salt and osmotic stresses on the expression of genes for the vacuolar H+-pyrophosphatase, H+-ATPase subunit A, and Na+/H+ antiporter from barley. J Exp Bot 55:585–594PubMedCrossRefGoogle Scholar
  15. Guo P, Baum M, Grando S, Ceccarelli S, Bai G, Li R, von Korff M, Varshney RK, Graner A, Valkoun J (2009) Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. J Exp Bot 60:3531–3544PubMedPubMedCentralCrossRefGoogle Scholar
  16. Gürel F, Öztürk NZ, Yörük E, Uçarlı C, Poyraz N (2016) Comparison of expression patterns of selected drought-responsive genes in barley (Hordeum vulgare L.) under shock-dehydration and slow drought treatments. Plant Growth Regul 80:183–193CrossRefGoogle Scholar
  17. Habte E, Müller LM, Shtaya M, Davis SJ, von Korff M (2014) Osmotic stress at the barley root affects expression of circadian clock genes in the shoot. Plant Cell Environ 37:1321–1337PubMedCrossRefGoogle Scholar
  18. Hasegawa PM (2013) Sodium (Na+) homeostasis and salt tolerance of plants. Environ Exp Bot 92:19–31CrossRefGoogle Scholar
  19. Hoagland DR, Arnon DI (1950) The water culture method for growing plants without soil. Calif AES Bull 347:1–32Google Scholar
  20. Hu L, Li H, Pang H, Fu J (2012) Responses of antioxidant gene, protein and enzymes to salinity stress in two genotypes of perennial ryegrass (Lolium perenne) differing in salt tolerance. J Plant Physiol 169:146–156PubMedCrossRefGoogle Scholar
  21. Jiang Q, Hu Z, Zhang H, Ma Y (2014a) Overexpression of GmDREB1 improves salt tolerance in transgenic wheat and leaf protein response to high salinity. Crop J 2:120–131CrossRefGoogle Scholar
  22. Jiang Q, Xu ZS, Wang F, Li MY, Ma J, Xiong AS (2014b) Effects of abiotic stresses on the expression of Lhcb1 gene and photosynthesis of Oenanthe javanica and Apium graveolens. Biol Plant 58:256–264CrossRefGoogle Scholar
  23. Kawasaki S, Borchert C, Deyholos M, Wang H, Brazille S, Kawaia K, Galbraitha D, Bohnerta HJ (2001) Gene expression profiles during the initial phase of salt stress in rice. Plant Cell 13:889–905PubMedPubMedCentralCrossRefGoogle Scholar
  24. König J, Baier M, Horling F, Kahmann U, Harris G, Schürmann P, Dietz KJ (2002) The plant-specific function of 2-Cys peroxiredoxin-mediated detoxification of peroxides in the redox-hierarchy of photosynthetic electron flux. PNAS USA 99(8):5738–5743PubMedCrossRefGoogle Scholar
  25. Langridge P, Fleury D (2011) Making the most of “omics” for crop breeding. Trends Biotechnol 29:33–40PubMedCrossRefGoogle Scholar
  26. Li H, Guo Q, Lan X, Zhou Q, Wei N (2014) Comparative expression analysis of five WRKY genes from Tibetan hulless barley under various abioticstresses between drought-resistant and sensitive genotype. Acta Physiol Plant 36:963973Google Scholar
  27. Mahajan S, Tuteja N (2005) Cold, salinity and drought stresses: an overview. Arch Biochem Biophys 444:139–158PubMedCrossRefGoogle Scholar
  28. Nayak SN, Balaji J, Upadhyaya HD, Hash CT, Kishor PBK, Chattopadhyay D, Rodriquez LM, Blair MW, Baum M, McNally K et al (2009) Isolation and sequence analysis of DREB2A homologues in three cereal and two legume species. Plant Sci 177:460–467CrossRefGoogle Scholar
  29. Ngara R, Ndimba BK (2014) Understanding the complex nature of salinity and drought-stress response in cereals using proteomics technologies. Proteomics 14:611–621PubMedCrossRefGoogle Scholar
  30. Öztürk ZN, Talamé V, Deyholos M, Michalowski CB, Galbraith DW, Gözükırmızı N, Tuberosa R, Bohnert HJ (2002) Monitoring large-scale changes in transcript abundance in drought-and salt-stressed barley. Plant Mol Biol 48:551–573CrossRefGoogle Scholar
  31. Pang Q, Chen S, Dai S, Wang Y, Chen Y, Yan X (2010) Comparative proteomics of salt tolerance in Arabidopsis thaliana and Thellungiella halophila. J Proteome Res 9:2584–2599PubMedCrossRefGoogle Scholar
  32. Parida AK, Das AB (2005) Salt tolerance and salinity effects on plants: a review. Ecotoxicol Environ Saf 60:324–349PubMedCrossRefGoogle Scholar
  33. Rushton PJ, Somssich IE, Ringler P, Shen QJ (2010) WRKY transcription factors. Trends Plant Sci 15:247–258PubMedCrossRefGoogle Scholar
  34. Saeed AI, Sharov V, White J, Li J, Liang W, Bhagabati N et al (2003) TM4: a free, open-source system for microarray data management and analysis. Biotechniques 34(2):374–378PubMedCrossRefGoogle Scholar
  35. Shelden MC, Roessner U (2013) Advances in functional genomics for investigating salinity stress tolerance mechanisms in cereals. Front Plant Sci 4:123PubMedPubMedCentralCrossRefGoogle Scholar
  36. Shinozaki K, Yamaguchi-Shinozaki K (2007) Gene networks involved in drought stress response and tolerance. J Exp Bot 58:221–227CrossRefGoogle Scholar
  37. Singh M, Kumar J, Singh S, Singh VP, Prasad SM (2015) Roles of osmoprotectants in improving salinity and drought tolerance in plants: a review. Rev Environ Sci Biol 14:407–426CrossRefGoogle Scholar
  38. Tavakkoli E, Fatehi F, Coventry S, Rengasamy P, McDonald GK (2011) Additive effects of Na+ and Cl ions on barley growth under salinity stress. J Exp Bot 62:2189–2203PubMedPubMedCentralCrossRefGoogle Scholar
  39. Tufan F, Uçarlı C, Tunalı B, Gürel F (2017) Analysis of early events in barley (Hordeum vulgare L.) roots in response to Fusarium culmorum infection. Eur J Plant Pathol 148:343–355CrossRefGoogle Scholar
  40. Ueda A, Kathiresan A, Inada M, Narita Y, Nakamura T, Shi W, Takabe T, Bennett J (2004) Osmotic stress in barley regulates expression of a different set of genes than salt stress does. J Exp Bot 55:2213–2218PubMedCrossRefGoogle Scholar
  41. UN DESA PD (2017) World Population Prospects: the 2017 revision, Key Findings and Advance Tables. Working Paper No. ESA/P/WP/248. New York, USAGoogle Scholar
  42. Vincent D, Ergul A, Bohlman MC, Tattersall EA, Tillett RL, Wheatley MD, Woolsey R, Quilici DR, Joets J, Schlauch K, Schooley DA, Cushman JC, Cramer GR (2007) Proteomic analysis reveals differences between Vitis vinifera L. cv. Chardonnay and cv. Cabernet Sauvignon and their responses to water deficit and salinity. J Exp Bot 58:1873–1892PubMedCrossRefGoogle Scholar
  43. Walia H, Wilson C, Wahid A, Condamine P, Cui X, Close TJ (2006) Expression analysis of barley (Hordeum vulgare L.) during salinity stress. Funct Integr Genom 6:143–156CrossRefGoogle Scholar
  44. Wu D, Shen Q, Qiu L, Han Y, Ye L, Jabeen Z, Shu Q, Zhang G (2014) Identification of proteins associated with ion homeostasis and salt tolerance in barley. Proteomics 14:1381–1392PubMedCrossRefGoogle Scholar
  45. Yang Z, Wu Y, Li Y, Ling HQ, Chu C (2009) OsMT1a, a type 1 metallothionein, plays the pivotal role in zinc homeostasis and drought tolerance in rice. Plant Mol Biol 70:219–229PubMedCrossRefGoogle Scholar
  46. Yang Y, He M, Zhu Z, Li S, Xu Y, Zhang C, Singer SD, Wang Y (2012) Identification of the dehydrin gene family from grapevine species and analysis of their responsiveness to various forms of abiotic and biotic stress. BMC Plant Biol 12:140PubMedPubMedCentralCrossRefGoogle Scholar
  47. Yousfi FE, Makhloufi E, Marande W, Ghorbel AW, Bouzayen M, Bergèse H (2017) Comparative analysis of WRKY genes potentially involved in salt stress responses in Triticum turgidum L. ssp. durum. Front Plant Sci 7:2034PubMedPubMedCentralCrossRefGoogle Scholar
  48. Zhang H, Han B, Wang T, Chen S, Li H, Zhang Y, Dai S (2012) Mechanisms of plant salt response: insights from proteomics. J Proteome Res 11:49–67PubMedCrossRefGoogle Scholar

Copyright information

© Korean Society for Plant Biotechnology 2019

Authors and Affiliations

  1. 1.Department of Molecular Biology and GeneticsIstanbul UniversityIstanbulTurkey

Personalised recommendations