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Expression of the sweet potato peroxidase gene swpa4 in Arabidopsis activates defense genes mediated by reactive oxygen species and nitric oxide

  • Yun-Hee KimEmail author
  • Byung-Wook Yun
  • Sang-Soo Kwak
Original Article
  • 25 Downloads

Abstract

Previously transgenic tobacco plants over-expressing the sweet potato peroxidase gene swpa4 exhibited increased tolerance to various stress conditions. Expression of swpa4 in tobacco also has a positive effect on expression of genes related to reactive oxygen species (ROS) and nitric oxide (NO). Expression levels of respiratory burst oxidase protein D (RBOHD), a major gene regulating ROS, and nitric oxide associated 1 (NOA1), a gene involved in NO generation, are significantly affected by peroxidase inhibitor treatments. In this study, swpa4 was expressed in the rbohD and noa1 knock-out mutants of Arabidopsis, as well as in wild-type plants. The transgenic plants showed increased levels of ROS and NO. Expression of swpa4 activated expression of genes related to ROS and NO. This suggested that swpa4 regulated H2O2 and NO homeostasis, thereby establishing a potential molecular link between the defense signaling pathways mediated by ROS and NO.

Keywords

Arabidopsis Nitric oxide Peroxidase Reactive oxygen species Sweet potato Tobacco 

Abbreviations

APX

Ascorbate peroxidase

CAT

Catalase

NOA

Nitric oxide associated

RBOH

Respiratory burst oxidase protein

RNS

Reactive nitrogen species

ROS

Reactive oxygen species

POD

Peroxidase

PR

Pathogenesis-related

SOD

Superoxide dismutase

Notes

Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2018R1A1A1A05018446), and the KRIBB Initiative Program.

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Copyright information

© Korean Society for Plant Biotechnology 2019

Authors and Affiliations

  1. 1.Department of Biology EducationGyeongsang National UniversityJinjuSouth Korea
  2. 2.Laboratory of Plant Functional Genomics, School of Applied BiosciencesKyungpook National UniversityDaeguSouth Korea
  3. 3.Plant Systems Engineering Research CenterKorea Research Institute of Bioscience and Biotechnology (KRIBB)DaejeonSouth Korea

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