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Plant Biotechnology Reports

, Volume 13, Issue 3, pp 285–292 | Cite as

Overexpression of the trehalose-6-phosphate phosphatase OsTPP3 increases drought tolerance in rice

  • Dagang Jiang
  • Weiting Chen
  • Jieer Gao
  • Fen Yang
  • Chuxiong ZhuangEmail author
Original Article

Abstract

Trehalose plays an important role in mediating stress responses in plants, and trehalose-6-phosphate synthases and trehalose-6-phosphate phosphatases are essential for trehalose biosynthesis. Here, we address the function of rice (Oryza sativa) OsTPP3. We analyzed the expression of OsTPP3 in different tissues and stress conditions, and generated OsTPP3-overexpressing rice plants. These plants showed a higher tolerance to simulated drought conditions (10% PEG treatment) than wild-type (WT) plants. Reverse-transcription quantitative PCR analysis indicated that transcript levels of genes related to stress responses and abscisic acid biosynthesis were significantly higher in the OsTPP3 overexpressors than in WT plants. These results highlight the importance of OsTPP3 in conferring drought tolerance in rice.

Keywords

Rice Trehalose OsTPP3 Drought tolerance ABA 

Notes

Acknowledgements

This study was supported by the National Natural Science Foundation of China (31100872) and Genetically Modified Breeding Major Projects (2016ZX08001-004).

Supplementary material

11816_2019_541_MOESM1_ESM.pdf (345 kb)
Supplementary material 1 (PDF 344 kb)

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Copyright information

© Korean Society for Plant Biotechnology 2019

Authors and Affiliations

  • Dagang Jiang
    • 1
  • Weiting Chen
    • 1
  • Jieer Gao
    • 1
  • Fen Yang
    • 1
  • Chuxiong Zhuang
    • 1
    Email author
  1. 1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life SciencesSouth China Agricultural UniversityGuangzhouChina

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