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Transgenic Arabidopsis plants expressing CsBCATs affect seed germination under abiotic stress conditions

  • Jeong Hwan Lee
  • Ji Hoon Han
  • Young-Cheon Kim
  • Young Hee Lee
  • Jeum Kyu Hong
  • Sanghyeob LeeEmail author
Short Communication
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Abstract

We investigated the responses of transgenic Arabidopsis plants that express cucumber branched-chain aminotransferases (CsBCATs) under abiotic or biotic stresses. The expression of CsBCAT3 and CsBCAT7 was dramatically increased by various abiotic treatments. The germination of CsBCAT3- or CsBCAT7-expressing Arabidopsis seeds was increased under salt and dehydration treatments, which was correlated with the increased or decreased expression levels of the germination-responsive genes. Although salicylic acid and jasmonic acid increased CsBCAT2 or CsBCAT7 expression, transgenic Arabidopsis plants that express CsBCAT2 or CsBCAT7 did not show the disease resistance to bacteria or fungus. Our results indicate that the CsBCATs affect seed germination of plants under abiotic stresses.

Keywords

Abiotic stress Biotic stress Branched-chain amino acid Branched-chain amino acid transferase CsBCATs Transgenic Arabidopsis plants 

Notes

Acknowledgements

This work was supported by grants from the National Research Foundation of Korea, and the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ01329601) of the Rural Development Administration, Republic of Korea. We wish to express our gratitude towards Youjin Jung, Daeun Choi, and Kwanuk Lee for technical assistance.

Supplementary material

11816_2019_515_MOESM1_ESM.docx (3.3 mb)
Supplementary material 1 (DOCX 3384 KB)

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Copyright information

© Korean Society for Plant Biotechnology 2019

Authors and Affiliations

  • Jeong Hwan Lee
    • 1
    • 2
  • Ji Hoon Han
    • 1
  • Young-Cheon Kim
    • 1
  • Young Hee Lee
    • 3
  • Jeum Kyu Hong
    • 3
  • Sanghyeob Lee
    • 1
    Email author
  1. 1.Department of Bioindustry and Bioresource Engineering, Plant Engineering Research InstituteSejong UniversitySeoulRepublic of Korea
  2. 2.Division of Life SciencesChonbuk National UniversityJeonjuRepublic of Korea
  3. 3.Laboratory of Plant Pathology and Protection, Department of Horticultural ScienceGyeongnam National University of Science and Technology (GNTech)JinjuRepublic of Korea

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