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Plant Biotechnology Reports

, Volume 12, Issue 5, pp 365–373 | Cite as

Characterization of high temperature-tolerant strains of Pyropia yezoensis

  • Yoon Ju Shin
  • Sung Ran Min
  • Da Yeon Kang
  • Jong-Min Lim
  • Eun-Jeong Park
  • Mi Sook Hwang
  • Dong-Woog Choi
  • Joon-Woo Ahn
  • Youn-Il Park
  • Won-Joong Jeong
Original Article
  • 119 Downloads

Abstract

High-temperature stress related to global warming reduces the growth and productivity of seaweeds. Thus, the development of new strains is urgently required for maintaining or even enhancing the productivity of useful seaweeds such as red alga Pyropia yezoenesis in an increasingly warmer sea environment. To develop competitive high-temperature-tolerant strains of P. yezoensis (Sugwawon no. 104), we screened libraries of gamma-irradiated strains and identified high-temperature-resistant (HTR) mutants. Our results showed that HTR-1 and HTR-2 grew well at higher temperatures that inhibited the growth of the wild-type strain. The efficiency of conchosporangium maturation and conchospore release of HTR-1 was similar to or higher than the wild-type strain. Moreover, thallus growth, pigment content, photosynthetic efficiency, and monospore release from the growing thallus in HTR-1 could be maintained even at high temperature. Taken together, our data demonstrate that HTR-1 may be suitable for industrial cultivation at sea, even at elevated temperatures.

Keywords

Pyropia yezoensis Conchocelis Gametophyte Gamma radiation mutants High temperature 

Notes

Acknowledgements

This work was supported by the Golden Seed Project, Ministry of Agriculture, Food, and Rural Affairs (MAFRA) and Rural Development Administration (RDA) (213008-05-2-SB820); the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Science and ICT (ABC-2011-0031343); and a Grant from KRIBB Research Initiative Program.

Supplementary material

11816_2018_499_MOESM1_ESM.docx (29 kb)
Supplementary material 1 (DOCX 29 KB)
11816_2018_499_MOESM2_ESM.pptx (3.5 mb)
Fig. S1 Sequences comparison for genetic deference between HTR-1 and wild type. a genomic PCR analysis of TRINITY_DN48859_c0_g2_i1 with specific primers (Table S1 and bold letters in b). b TRINITY_DN48859_c0_g2_i1 in HTR-1 was identified from transcriptomes based comparing and variant calling between wild type and mutant. Red letters represent 6 nts insertion in HTR-1 compared with wild-type sequence corresponding for TRINITY_DN48859_c0_g2_i1. Bold letters represent primers used for genomic PCR. Fig. S2 Efficiency of conchosporangium maturation of HTR-1 and HTR-2 mutant and wild-type strains following incubation at different culture conditions. a Fluorescence micrographs of conchosporangium. Different photoperiods of light and darkness below temperatures represent in parentheses. b Efficiency of conchosporangium maturation. Conchocelis from each strain (2 mg) were cultured for 8 weeks to mature the conchosporangium. Error bars represent the standard deviation of ten replicates. Scale bars represent 100 µm. Fig. S3 Fluorescence micrographs of monospores and germinated monospores on filament in the wild-type (WT) and mutant (HTR-1 and HTR-2) strains. Scale bars represent 100 µm. (PPTX 3557 KB)

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Copyright information

© Korean Society for Plant Biotechnology and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Plant Systems Engineering Research CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonSouth Korea
  2. 2.Department of Biological SciencesChungnam National UniversityDaejeonSouth Korea
  3. 3.Seaweed Research CenterNational Fisheries Research and Development InstituteMokpoSouth Korea
  4. 4.Department of Biology EducationChonnam National UniversityGwangjuSouth Korea
  5. 5.Advanced Radiation Technology InstituteKorea Atomic Energy Research InstituteJeongeupSouth Korea

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