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Journal of Ocean University of China

, Volume 18, Issue 6, pp 1443–1450 | Cite as

Characterization and Functional Study on Octopus ocellatus Interleukin-17

  • Zan Li
  • Tingting Fan
  • Xintian Liu
  • Xiumei Liu
  • Weijun Wang
  • Qianqian Wang
  • Lunhe You
  • Lei Wang
  • Xiumei Wei
  • Jianmin YangEmail author
Article
  • 7 Downloads

Abstract

Interleukin-17 (IL-17), a prototype member of IL-17 family, plays an important role in defending against extracellular pathogens as a pro-inflammatory cytokine. The function and distribution of IL-17 have been extensively studied in many vertebrates. However, few study has focused on the role of IL-17 in invertebrates, especially in mollusks. In this study, an IL-17 homolog was identified in Octopus ocellatus, which was designated as OoIL-17. The phylogenetic analysis showed that OoIL-17 is clustered well with other invertebrate IL-17, indicating it is highly similar with the IL-17 of other invertebrates. The expression of OoIL-17 gene was analyzed with qRT-PCR in a variety of healthy tissues and the hemocytes infected with Vibro anguillarum or Micrococcus luteus. The mRNA of OoIL-17 gene is constitutively expressed at different levels in all examined tissues of healthy O. ocellatus, including mantle, stomach, hemocytes, muscle, gonad, hepatopancreas, systemic heart and gill. The lowest expression was observed in mantle while the highest was observed in hepatopancreas. The expression level of OoIL-17 gene is significantly up-regulated in O. ocellatus hemocytes upon infection with V. anguillarum and M. luteus, indicating its active involvement in the host immune response against bacterial pathogens. The results laid the foundation for further understanding the innate immune mechanisms of IL-17 in O. ocellatus and mollusks.

Key words

IL-17 Octopus ocellatus Vibro anguillarum Micrococcus luteus 

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Notes

Acknowledgements

This research was supported by the earmarked fund for the Modern Agro-Industry Technology Research System (No. CARS-49), the Natural Science Foundation of Shandong Province (No. ZR2019BC052), and the Marine and Fisheries Science and Technology Innovation Program of Shan- dong Province (No. 2017YY04).

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Copyright information

© Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019

Authors and Affiliations

  • Zan Li
    • 1
  • Tingting Fan
    • 1
  • Xintian Liu
    • 2
  • Xiumei Liu
    • 3
  • Weijun Wang
    • 1
  • Qianqian Wang
    • 1
  • Lunhe You
    • 1
  • Lei Wang
    • 4
  • Xiumei Wei
    • 5
  • Jianmin Yang
    • 1
    Email author
  1. 1.School of AgricultureLudong UniversityYantaiChina
  2. 2.Weihai Fisheries Technology Extension CenterWeihaiChina
  3. 3.College of Life SciencesYantai UniversityYantaiChina
  4. 4.College of Life SciencesLudong UniversityYantaiChina
  5. 5.School of Life SciencesEast China Normal UniversityShanghaiChina

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