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Analysis of antibiotic resistance of Escherichia coli isolated from the Yitong River in North-east China

  • Yangyang Yu
  • Xiaolin Zhu
  • Guanlan Wu
  • Chengzhi Wang
  • Xing YuanEmail author
Research Article
  • 1 Downloads
Part of the following topical collections:
  1. Special Issue—Environmental Antibiotics and Antibiotic Resistance

Abstract

The Yitong River is one of the largest secondary tributaries of the Songhua River. The area where the Yitong River flows is densely populated and contains the livestock and poultry breeding areas of northeast China. These areas introduce a high risk of antibiotic contamination. In this study, the concentrations of four types of typical antibiotics including quinolones, tetracyclines, sulfonamides, and trimethoprim were determined by solid phase extraction-high performance liquid chromatography. The antibiotic resistance of Escherichia coli caused by antibiotic pollution was investigated. The concentration of total coliforms in the Yitong River was detected by the plate counting method. The antibiotic resistance of E. coli to quinolones, tetracyclines, sulfonamides, and trimethoprim was analyzed by the Kirby-Bauer method. The results showed that the concentration of total coliforms in the summer was higher than that in the spring. There was a seasonal difference in the resistance rate of E. coli to antibiotics except trimethoprim. The antibiotic resistance to fluoroquinolones was relatively low. The resistance rate to tetracyclines was higher during the summer. Moreover, resistance to several antibiotics was observed in all sections. This study provides basic data for research on pollution characteristics and prevention of antibiotic exposure in rivers.

Keywords

Yitong River Coliform bacteria Antibiotic resistance Escherichia coli 

Notes

Acknowledgements

This work was supported by the Special S&T Project on Treatment and Control of Water Pollution (No. 2014ZX07201-011-008), the National Natural Science Foundation of China (Grant No. 51809044), Environmental Protection Project of Ecology and Environment Department of Jilin Province (2018-10), the China Postdoctoral Science Foundation (2018M630314), the Fundamental Research Funds for the Central Universities (No. 2412018QD020), and the Jilin Province Science and Technology Development Projects (No. 20190103136JH).

Supplementary material

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Copyright information

© Higher Education Press and Springer—Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Yangyang Yu
    • 1
  • Xiaolin Zhu
    • 1
  • Guanlan Wu
    • 1
  • Chengzhi Wang
    • 1
  • Xing Yuan
    • 1
    Email author
  1. 1.School of EnvironmentNortheast Normal UniversityChangchunChina

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