Analysis of antibiotic resistance of Escherichia coli isolated from the Yitong River in North-east China

  • Yangyang Yu
  • Xiaolin Zhu
  • Guanlan Wu
  • Chengzhi Wang
  • Xing YuanEmail author
Research Article
Part of the following topical collections:
  1. Special Issue—Environmental Antibiotics and Antibiotic Resistance


The Yitong River is one of the largest secondary tributaries of the Songhua River. The area where the Yitong River flows is densely populated and contains the livestock and poultry breeding areas of northeast China. These areas introduce a high risk of antibiotic contamination. In this study, the concentrations of four types of typical antibiotics including quinolones, tetracyclines, sulfonamides, and trimethoprim were determined by solid phase extraction-high performance liquid chromatography. The antibiotic resistance of Escherichia coli caused by antibiotic pollution was investigated. The concentration of total coliforms in the Yitong River was detected by the plate counting method. The antibiotic resistance of E. coli to quinolones, tetracyclines, sulfonamides, and trimethoprim was analyzed by the Kirby-Bauer method. The results showed that the concentration of total coliforms in the summer was higher than that in the spring. There was a seasonal difference in the resistance rate of E. coli to antibiotics except trimethoprim. The antibiotic resistance to fluoroquinolones was relatively low. The resistance rate to tetracyclines was higher during the summer. Moreover, resistance to several antibiotics was observed in all sections. This study provides basic data for research on pollution characteristics and prevention of antibiotic exposure in rivers.


Yitong River Coliform bacteria Antibiotic resistance Escherichia coli 



This work was supported by the Special S&T Project on Treatment and Control of Water Pollution (No. 2014ZX07201-011-008), the National Natural Science Foundation of China (Grant No. 51809044), Environmental Protection Project of Ecology and Environment Department of Jilin Province (2018-10), the China Postdoctoral Science Foundation (2018M630314), the Fundamental Research Funds for the Central Universities (No. 2412018QD020), and the Jilin Province Science and Technology Development Projects (No. 20190103136JH).

Supplementary material


  1. Al-Badaii F, Shuhaimi-Othman M (2015). Water pollution and its impact on the prevalence of antibiotic-resistant E. coli and total coliform bacteria: a study of the Semenyih River, Peninsular Malaysia. Water Quality, Exposure and Health, 7(3): 319–330CrossRefGoogle Scholar
  2. Chidamba L, Cilliers E, Bezuidenhout C C (2016). Spatial and temporal variations in pollution indicator bacteria in the Lower Vaal River, South Africa. Water Environment Research, 88(11): 2142–2149CrossRefGoogle Scholar
  3. CLSI (2013). Performance standards for antimicrobial susceptibility testing; Twenty-second informational supplement. CLSI document M100-S23. Wayne, PA: Clinical and Laboratory Standards InstituteGoogle Scholar
  4. Cullen I M, Manecksha R P, McCullagh E, Ahmad S, O’Kelly F, Flynn R, McDermott T E, Murphy P, Grainger R, Fennell J P, Thornhill J A (2013). An 11-year analysis of the prevalent uropathogens and the changing pattern of Escherichia coli antibiotic resistance in 38,530 community urinary tract infections, Dublin 1999–2009. Irish Journal of Medical Science, 182(1): 81–89CrossRefGoogle Scholar
  5. da Silva T F B X, Ramos D T, Dziedzic M, de Oliveira C M R, de Vasconcelos E C (2011). Microbiological quality and antibiotic resistance analysis of a brazilian water supply source. Water, Air, and Soil Pollution, 218(1–4): 611–618CrossRefGoogle Scholar
  6. Dong D, Zhang L, Liu S, Guo Z, Hua X (2016). Antibiotics in water and sediments from Liao River in Jilin Province, China: Occurrence, distribution, and risk assessment. Environmental Earth Sciences, 75(16): 1202CrossRefGoogle Scholar
  7. Du J, Zhao H, Liu S, Xie H, Wang Y, Chen J (2017). Antibiotics in the coastal water of the South Yellow Sea in China: Occurrence, distribution and ecological risks. Science of the Total Environment, 595: 521–527CrossRefGoogle Scholar
  8. Fram M S, Belitz K (2011). Occurrence and concentrations of pharmaceutical compounds in groundwater used for public drinking-water supply in California. Science of the Total Environment, 409(18): 3409–3417CrossRefGoogle Scholar
  9. Gao L, Shi Y, Li W, Niu H, Liu J, Cai Y (2012). Occurrence of antibiotics in eight sewage treatment plants in Beijing, China. Chemosphere, 86(6): 665–671CrossRefGoogle Scholar
  10. Gilroy E A, Klinck J S, Campbell S D, McInnis R, Gillis P L, de Solla S R (2014). Toxicity and bioconcentration of the pharmaceuticals moxifloxacin, rosuvastatin, and drospirenone to the unionid mussel Lampsilis siliquoidea. Science of the Total Environment, 487: 537–544CrossRefGoogle Scholar
  11. Gonzâlez-Pleiter M, Gonzalo S, Rodea-Palomares I, Leganés F, Rosal R, Boltes K, Marco E, Fernández-Piñas F (2013). Toxicity of five antibiotics and their mixtures towards photosynthetic aquatic organisms: Implications for environmental risk assessment. Water Research, 47(6): 2050–2064CrossRefGoogle Scholar
  12. Gulkowska A, Leung H W, So M K, Taniyasu S, Yamashita N, Yeung L W, Richardson B J, Lei A P, Giesy J P, Lam P K S (2008). Removal of antibiotics from wastewater by sewage treatment facilities in Hong Kong and Shenzhen, China. Water Research, 42(1–2): 395–403CrossRefGoogle Scholar
  13. Guyomard-Rabenirina S, Dartron C, Falord M, Sadikalay S, Ducat C, Richard V, Breurec S, Gros O, Talarmin A (2017). Resistance to antimicrobial drugs in different surface waters and wastewaters of Guadeloupe. PLoS One, 12(3): e0173155CrossRefGoogle Scholar
  14. Halling-Sørensen B, Nors Nielsen S, Lanzky P F, Ingerslev F, Holten Lützhøft H C, Jørgensen S E (1998). Occurrence, fate and effects of pharmaceutical substances in the environment: A review. Chemosphere, 36(2): 357–393CrossRefGoogle Scholar
  15. Hawkes M, Barton M, Conly J, Nicolle L, Barry C, Ford-Jones E L (2007). Community-associated MRSA: Superbug at our doorstep. Canadian Medical Association Journal, 176(1): 54–56CrossRefGoogle Scholar
  16. He X, Deng M, Wang Q, Yany Y, Yang Y, Nie X (2016). Residues and health risk assessment of quinolones and sulfonamides in cultured fish from Pearl River Delta, China. Aquaculture, 458: 38–46CrossRefGoogle Scholar
  17. Hu Y, Yan X, Shen Y, Di M, Wang J (2018). Antibiotics in surface water and sediments from Hanjiang River, Central China: Occurrence, behavior and risk assessment. Ecotoxicology and Environmental Safety, 157: 150–158CrossRefGoogle Scholar
  18. Jia A, Wan Y, Xiao Y, Hu J (2012). Occurrence and fate of quinolone and fluoroquinolone antibiotics in a municipal sewage treatment plant. Water Research, 46(2): 387–394CrossRefGoogle Scholar
  19. Kim G, Choi E, Lee D (2005). Diffuse and point pollution impacts on the pathogen indicator organism level in the Geum River, Korea. Science of the Total Environment, 350(1–3): 94–105CrossRefGoogle Scholar
  20. Kim J W, Ishibashi H, Yamauchi R, Ichikawa N, Takao Y, Hirano M, Koga M, Arizono K (2009). Acute toxicity of pharmaceutical and personal care products on freshwater crustacean (Thamnocephalus platyurus) and fish (Oryzias latipes). The Journal of Toxicological Sciences, 34(2): 227–232CrossRefGoogle Scholar
  21. Kim S C, Carlson K (2007). Temporal and spatial trends in the occurrence of human and veterinary antibiotics in aqueous and river sediment matrices. Environmental Science & Technology, 41(1): 50–57CrossRefGoogle Scholar
  22. Kim Y, Lee K B, Choi K (2016). Effect of runoff discharge on the environmental levels of 13 veterinary antibiotics: A case study of Han River and Kyungahn Stream, South Korea. Marine Pollution Bulletin, 107(1): 347–354CrossRefGoogle Scholar
  23. Le T X, Munekage Y (2004). Residues of selected antibiotics in water and mud from shrimp ponds in mangrove areas in Viet Nam. Marine Pollution Bulletin, 49(11–12): 922–929CrossRefGoogle Scholar
  24. Li S, Shi W, Li H, Xu N, Zhang R, Chen X, Sun W, Wen D, He S, Pan J, He Z, Fan Y (2018). Antibiotics in water and sediments of rivers and coastal area of Zhuhai City, Pearl River estuary, south China. Science of the Total Environment, 636: 1009–1019CrossRefGoogle Scholar
  25. Liang X, Chen B, Nie X, Shi Z, Huang X, Li X (2013). The distribution and partitioning of common antibiotics in water and sediment of the Pearl River Estuary, south China. Chemosphere, 92(11): 1410–1416CrossRefGoogle Scholar
  26. Liu J, Lu G, Wu D, Yan Z (2014). A multi-biomarker assessment of single and combined effects of norfloxacin and sulfamethoxazole on male goldfish (Carassius auratus). Ecotoxicology and Environmental Safety, 102: 12–17CrossRefGoogle Scholar
  27. Liu Q, Li M, Liu X, Zhang Q, Liu R, Wang Z, Shi X, Quan J, Shen X, Zhang F (2018). Removal of sulfamethoxazole and trimethoprim from reclaimed water and the biodegradation mechanism. Frontiers of Environmental Science & Engineering, 12(6): 6CrossRefGoogle Scholar
  28. Łuczkiewicz A, Jankowska K, Fudala-Książek S, Olałczuk-Neyman K (2010). Antimicrobial resistance of fecal indicators in municipal wastewater treatment plant. Water Research, 44(17): 5089–5097CrossRefGoogle Scholar
  29. Martins da Costa P, Vaz-Pires P, Bernardo F (2006). Antimicrobial resistance in Enterococcus spp. isolated in inflow, effluent and sludge from municipal sewage water treatment plants. Water Research, 40(8): 1735–1740CrossRefGoogle Scholar
  30. Morris A, Kellner J D, Low D E (1998). The superbugs: Evolution, dissemination and fitness. Current Opinion in Microbiology, 1(5): 524–529CrossRefGoogle Scholar
  31. Pan M, Chu L M (2018). Occurrence of antibiotics and antibiotic resistance genes in soils from wastewater irrigation areas in the Pearl River Delta region, southern China. Science of the Total Environment, 624: 145–152CrossRefGoogle Scholar
  32. Pintadoherrera M G, Wang C, Lu J, Chang Y, Chen W, Li X, Lara-Martín P A (2017). Distribution, mass inventories, and ecological risk assessment of legacy and emerging contaminants in sediments from the Pearl River Estuary in China. Journal of Hazardous Materials, 323 (Pt A): 128–138CrossRefGoogle Scholar
  33. Pouzaud F, Dutot M, Martin C, Debray M, Warnet J M, Rat P (2006). Age-dependent effects on redox status, oxidative stress, mitochondrial activity and toxicity induced by fluoroquinolones on primary cultures of rabbit tendon cells. Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, 143(2): 0–241CrossRefGoogle Scholar
  34. Riva F, Zuccato E, Castiglioni S (2015). Prioritization and analysis of pharmaceuticals for human use contaminating the aquatic ecosystem in Italy. Journal of Pharmaceutical and Biomedical Analysis, 106: 71–78CrossRefGoogle Scholar
  35. Roberts P H, Thomas K V (2006). The occurrence of selected pharmaceuticals in wastewater effluent and surface waters of the lower Tyne catchment. Science of the Total Environment, 356(1–3): 143–153CrossRefGoogle Scholar
  36. Seoane M, Rioboo C, Herrero C, Cid Á (2014). Toxicity induced by three antibiotics commonly used in aquaculture on the marine microalga Tetraselmis suecica (Kylin) Butch. Marine Environmental Research, 101: 1–7CrossRefGoogle Scholar
  37. Song X, Liu R, Chen L, Kawagishi T (2017). Comparative experiment on treating digested piggery wastewater with a biofilm MBR and conventional MBR: Simultaneous removal of nitrogen and anti-biotics. Frontiers of Environmental Science & Engineering, 11(2): 11CrossRefGoogle Scholar
  38. Wollenberger L, Halling-Sørensen B, Kusk K O (2000). Acute and chronic toxicity of veterinary antibiotics to Daphnia magna. Chemosphere, 40(7): 723–730CrossRefGoogle Scholar
  39. Wu D, Huang Z, Yang K, Graham D, Xie B (2015). Relationships between antibiotics and antibiotic resistance gene levels in municipal solid waste leachates in Shanghai, China. Environmental Science & Technology, 49(7): 4122–4128CrossRefGoogle Scholar
  40. Wu M H, Que C J, Xu G, Sun Y F, Ma J, Xu H, Sun R, Tang L (2016). Occurrence, fate and interrelation of selected antibiotics in sewage treatment plants and their receiving surface water. Ecotoxicology and Environmental Safety, 132: 132–139CrossRefGoogle Scholar
  41. Wu X L, Xiang L, Yan Q Y, Jiang Y N, Li Y W, Huang X P, Li H, Cai Q Y, Mo C H (2014). Distribution and risk assessment of quinolone antibiotics in the soils from organic vegetable farms of a subtropical city, southern China. Science of the Total Environment, 487(1): 399–406CrossRefGoogle Scholar
  42. Xiao H (2014). Pharmacokinetic of Compound Sulfadiazine in GIFT. Dissertation for the Master Degree. Shanghai: Shanghai Ocean University (in Chinese)Google Scholar
  43. Xu W, Zhang G, Li X, Zou S, Li P, Hu Z, Li J (2007). Occurrence and elimination of antibiotics at four sewage treatment plants in the Pearl River Delta (PRD), South China. Water Research, 41(19): 4526–4534CrossRefGoogle Scholar
  44. Yao L, Wang Y, Tong L, Li Y, Deng Y, Guo W, Gan Y (2015). Seasonal variation of antibiotics concentration in the aquatic environment: A case study at Jianghan Plain, central China. Science of the Total Environment, 527–528: 56–64CrossRefGoogle Scholar
  45. Yu Z, Jiang L, Yin D (2011). Behavior toxicity to Caenorhabditis elegans transferred to the progeny after exposure to sulfamethoxazole at environmentally relevant concentrations. Journal of Environmental Sciences-China, 23(2): 294–300CrossRefGoogle Scholar
  46. Zhang S H, Lv X, Han B, Gu X, Wang P F, Wang C, He Z (2015). Prevalence of antibiotic resistance genes in antibiotic-resistant Escherichia coli isolates in surface water of Taihu Lake Basin, China. Environmental Science and Pollution Research International, 22(15): 11412–11421CrossRefGoogle Scholar

Copyright information

© Higher Education Press and Springer—Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Yangyang Yu
    • 1
  • Xiaolin Zhu
    • 1
  • Guanlan Wu
    • 1
  • Chengzhi Wang
    • 1
  • Xing Yuan
    • 1
    Email author
  1. 1.School of EnvironmentNortheast Normal UniversityChangchunChina

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