Analysis of antibiotic resistance of Escherichia coli isolated from the Yitong River in North-east China
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The Yitong River is one of the largest secondary tributaries of the Songhua River. The area where the Yitong River flows is densely populated and contains the livestock and poultry breeding areas of northeast China. These areas introduce a high risk of antibiotic contamination. In this study, the concentrations of four types of typical antibiotics including quinolones, tetracyclines, sulfonamides, and trimethoprim were determined by solid phase extraction-high performance liquid chromatography. The antibiotic resistance of Escherichia coli caused by antibiotic pollution was investigated. The concentration of total coliforms in the Yitong River was detected by the plate counting method. The antibiotic resistance of E. coli to quinolones, tetracyclines, sulfonamides, and trimethoprim was analyzed by the Kirby-Bauer method. The results showed that the concentration of total coliforms in the summer was higher than that in the spring. There was a seasonal difference in the resistance rate of E. coli to antibiotics except trimethoprim. The antibiotic resistance to fluoroquinolones was relatively low. The resistance rate to tetracyclines was higher during the summer. Moreover, resistance to several antibiotics was observed in all sections. This study provides basic data for research on pollution characteristics and prevention of antibiotic exposure in rivers.
KeywordsYitong River Coliform bacteria Antibiotic resistance Escherichia coli
This work was supported by the Special S&T Project on Treatment and Control of Water Pollution (No. 2014ZX07201-011-008), the National Natural Science Foundation of China (Grant No. 51809044), Environmental Protection Project of Ecology and Environment Department of Jilin Province (2018-10), the China Postdoctoral Science Foundation (2018M630314), the Fundamental Research Funds for the Central Universities (No. 2412018QD020), and the Jilin Province Science and Technology Development Projects (No. 20190103136JH).
- CLSI (2013). Performance standards for antimicrobial susceptibility testing; Twenty-second informational supplement. CLSI document M100-S23. Wayne, PA: Clinical and Laboratory Standards InstituteGoogle Scholar
- Cullen I M, Manecksha R P, McCullagh E, Ahmad S, O’Kelly F, Flynn R, McDermott T E, Murphy P, Grainger R, Fennell J P, Thornhill J A (2013). An 11-year analysis of the prevalent uropathogens and the changing pattern of Escherichia coli antibiotic resistance in 38,530 community urinary tract infections, Dublin 1999–2009. Irish Journal of Medical Science, 182(1): 81–89CrossRefGoogle Scholar
- Gonzâlez-Pleiter M, Gonzalo S, Rodea-Palomares I, Leganés F, Rosal R, Boltes K, Marco E, Fernández-Piñas F (2013). Toxicity of five antibiotics and their mixtures towards photosynthetic aquatic organisms: Implications for environmental risk assessment. Water Research, 47(6): 2050–2064CrossRefGoogle Scholar
- Kim J W, Ishibashi H, Yamauchi R, Ichikawa N, Takao Y, Hirano M, Koga M, Arizono K (2009). Acute toxicity of pharmaceutical and personal care products on freshwater crustacean (Thamnocephalus platyurus) and fish (Oryzias latipes). The Journal of Toxicological Sciences, 34(2): 227–232CrossRefGoogle Scholar
- Pintadoherrera M G, Wang C, Lu J, Chang Y, Chen W, Li X, Lara-Martín P A (2017). Distribution, mass inventories, and ecological risk assessment of legacy and emerging contaminants in sediments from the Pearl River Estuary in China. Journal of Hazardous Materials, 323 (Pt A): 128–138CrossRefGoogle Scholar
- Pouzaud F, Dutot M, Martin C, Debray M, Warnet J M, Rat P (2006). Age-dependent effects on redox status, oxidative stress, mitochondrial activity and toxicity induced by fluoroquinolones on primary cultures of rabbit tendon cells. Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, 143(2): 0–241CrossRefGoogle Scholar
- Xiao H (2014). Pharmacokinetic of Compound Sulfadiazine in GIFT. Dissertation for the Master Degree. Shanghai: Shanghai Ocean University (in Chinese)Google Scholar