Isolation and application of an ibuprofen-degrading bacterium to a biological aerated filter for the treatment of micro-polluted water

  • Bingjie Xu
  • Gang XueEmail author
  • Xing Yang
Research Article


Ibuprofen (IBU) is widely used in the world as anti-inflammatory drug, which posed health risk to the environment. A bacterium capable of degrading IBU was isolated from activated sludge in a sewage treatment plant. According to its morphological, physiologic, and biochemical characteristics, as well as 16S rRNA sequence analysis, the strain was identified as Serratia marcescens BL1 (BL1). Degradation of IBU required the presence of primary substrate. After a five-day cultivation with yeast powder at 30°C and pH 7, the highest degradation (93.47%±2.37%) was achieved. The process of BL1 degrading IBU followed first-order reaction kinetics. The BL1 strain was applied to a small biological aerated filter (BAF) device to form a biofilm with activated sludge. IBU removal by the BAF was consistent with the results of static tests. The removal of IBU was 32.01% to 44.04% higher than for a BAF without BL1. The indigenous bacterial community was able to effectively remove CODMn (permanganate index) and ammonia nitrogen in the presence of BL1.


Ibuprofen Biological aerated filter Degrading bacterium Serratia marcescens 



This work was funded by the National Natural Science Foundation of China (Grant Nos. 21767013 and 51741805) and the Natural Science Foundation of Jiangxi Province (No. 20151BA B213018).


  1. Abu Hasan H, Sheikh Abdullah S R, Al-Attabi A W N, Nash D A H, Anuar N, Abd. Rahman N, Sulistiyaning Titah H (2016). Removal of ibuprofen, ketoprofen, COD and nitrogen compounds from pharmaceutical wastewater using aerobic suspension-sequencing batch reactor (ASSBR). Separation and Purification Technology, 157(1): 215–221CrossRefGoogle Scholar
  2. Ali I, Singh P, Aboul-Enein H Y, Sharma B (2009). Chiral analysis of ibuprofen residues in water and sediment. Analytical Letters, 42(12): 1747–1760CrossRefGoogle Scholar
  3. Baghapour M A, Shirdarreh M R, Faramarzian M (2015). Amoxicillin removal from aqueous solutions using submerged biological aerated filter. Desalination and Water Treatment, 54(3): 790–801CrossRefGoogle Scholar
  4. Chu H, Cao D, Dong B, Qiang Z (2010). Bio-diatomite dynamic membrane reactor for micro-polluted surface water treatment. Water Research, 44(5): 1573–1579CrossRefGoogle Scholar
  5. Cycon M, Zmijowska A, Wójcik M, Piotrowska-Seget Z (2013). Biodegradation and bioremediation potential of diazinon-degrading Serratia marcescens to remove other organophosphorus pesticides from soils. Journal of Environmental Management, 117(1): 7–16CrossRefGoogle Scholar
  6. de Sousa D N, Mozeto A A, Carneiro R L, Fadini P S (2014). Electrical conductivity and emerging contaminant as markers of surface freshwater contamination by wastewater. Science of the Total Environment, 484(1): 19–26CrossRefGoogle Scholar
  7. Ferrando-Climent L, Collado N, Buttiglieri G, Gros M, Rodriguez-Roda I, Rodriguez-Mozaz S, Barceló D (2012). Comprehensive study of ibuprofen and its metabolites in activated sludge batch experiments and aquatic environment. Science of the Total Environment, 438(1): 404–413CrossRefGoogle Scholar
  8. Gao P, Wei X, Gu C, Wu X, Xue G, Shi W, Sun W (2015). Isolation and characterization of an erythromycin-degrading strain and application for bioaugmentation in a biological aerated filter. Water, Air, & Soil Pollution, 226(6): 190CrossRefGoogle Scholar
  9. Guo H, Yao J, Chen H, Wang J, Masakorala K, Jin Y, Richnow H H, Blake R E (2012). Substrate interactions during biodegradation of benzene/alkylbenzene mixtures by Rhodococcus sp. ustb-1. International Biodeterioration & Biodegradation, 75(1): 124–130CrossRefGoogle Scholar
  10. Halling-Sørensen B, Nors Nielsen S, Lanzky P F, Ingerslev F, Holten Lützhøft H C, Jørgensen S E (1998). Occurrence, fate and effects of pharmaceutical substances in the environment–a review. Chemosphere, 36(2): 357–393CrossRefGoogle Scholar
  11. Han S, Choi K, Kim J, Ji K, Kim S, Ahn B, Yun J, Choi K, Khim J S, Zhang X, Giesy J P (2010). Endocrine disruption and consequences of chronic exposure to ibuprofen in Japanese medaka (Oryzias latipes) and freshwater cladocerans Daphnia magna and Moina macrocopa. Aquatic Toxicology (Amsterdam, Netherlands), 98(3): 256–264CrossRefGoogle Scholar
  12. He S, Wang J, Ye L, Zhang Y, Yu J (2014). Removal of diclofenac from surface water by electron beam irradiation combined with a biological aerated filter. Radiation Physics and Chemistry, 105(1): 104–108CrossRefGoogle Scholar
  13. Hoseinzadeh E, Rezaee A, Hossini H (2016). Biological nitrogen removal in moving bed biofilm reactor using ibuprofen as carbon source. Water, Air, and Soil Pollution, 227(2): 46CrossRefGoogle Scholar
  14. Li X, Wang Y, Yuan S, Li Z, Wang B, Huang J, Deng S, Yu G (2014). Degradation of the anti-inflammatory drug ibuprofen by electroperoxone process. Water Research, 63(1): 81–93CrossRefGoogle Scholar
  15. Li Y, Wu B, Zhu G, Liu Y, Ng W J, Appan A, Tan S K (2016). Highthroughput pyrosequencing analysis of bacteria relevant to cometabolic and metabolic degradation of ibuprofen in horizontal subsurface flow constructed wetlands. Science of the Total Environment, 562(1): 604–613CrossRefGoogle Scholar
  16. Liang Y, Zeng F, Qiu G, Lu X, Liu X, Gao H (2009). Co-metabolic degradation of dimethoate by Raoultella sp. X1. Biodegradation, 20 (3): 363–373CrossRefGoogle Scholar
  17. Lin Y, Li D, Zeng S, He M (2016). Changes of microbial composition during wastewater reclamation and distribution systems revealed by high-throughput sequencing analyses. Frontiers of Environmental Science & Engineering, 10(3): 539–547CrossRefGoogle Scholar
  18. Lindholm-Lehto P C, Ahkola H S J, Knuutinen J S, Herve S H (2016). Widespread occurrence and seasonal variation of pharmaceuticals in surface waters and municipal wastewater treatment plants in central Finland. Environmental Science and Pollution Research International, 23(8): 7985–7997CrossRefGoogle Scholar
  19. Londoño Y A, Peñuela G A (2015). Biological removal of different concentrations of ibuprofen and methylparaben in a sequencing batch reactor (SBR). Water, Air, and Soil Pollution, 226(12): 393CrossRefGoogle Scholar
  20. Marchlewicz A, Domaradzka D, Guzik U, Wojcieszynska D (2016). Bacillus thuringiensis B1(2015b) is a gram-positive bacteria able to degrade naproxen and ibuprofen. Water, Air & Soil Pollution, 227(6): 197CrossRefGoogle Scholar
  21. Marchlewicz A, Guzik U, Hupert-Kocurek K, Nowak A, Wilczynska S, Wojcieszynska D (2017). Toxicity and biodegradation of ibuprofen by Bacillus thuringiensis B1(2015b). Environmental Science and Pollution Research International, 24(8): 7572–7584CrossRefGoogle Scholar
  22. Ministry of Environmental Protection of the People’s Republic of China (1989). Water quality-Determination of permanganate index. Available online at (accessed July 19, 2018) (in Chinese)
  23. Ministry of Environmental Protection of the People’s Republic of China (2009). Water quality-Determination of ammonia nitrogen-Nessler’s reagent spectrophotometry. Available online at (accessed July 19, 2018) (in Chinese)
  24. Murdoch R W, Hay A G (2013). Genetic and chemical characterization of ibuprofen degradation by Sphingomonas Ibu-2. Microbiology, 159 (Pt_3): 621–632CrossRefGoogle Scholar
  25. Paíga P, Santos L H M L M, Amorim C G, Araújo A N, Montenegro M C, Pena A, Delerue-Matos C (2013). Pilot monitoring study of ibuprofen in surface waters of north of Portugal. Environmental Science and Pollution Research International, 20(4): 2410–2420CrossRefGoogle Scholar
  26. Pakshirajan K, Chugh D, Saravanan P (2008). Feasibility of m-cresol degradation using an indigenous mixed microbial culture with glucose as co-substrate. Clean Technologies and Environmental Policy, 10(3): 303–308CrossRefGoogle Scholar
  27. Parolini M, Binelli A, Provini A (2011). Chronic effects induced by ibuprofen on the freshwater bivalve Dreissena polymorpha. Ecotoxicology and Environmental Safety, 74(6): 1586–1594CrossRefGoogle Scholar
  28. Phan H V, Hai F I, Mcdonald J A, Khan S J, Zhang R, Price W E, Broeckmann A, Nghiem L D (2015). Nutrient and trace organic contaminant removal from wastewater of a resort town: comparison between a pilot and a full scale membrane bioreactor. International Biodeterioration & Biodegradation, 102(1): 40–48CrossRefGoogle Scholar
  29. Pietrini F, Di Baccio D, Aceña J, Pérez S, Barceló D, Zacchini M (2015). Ibuprofen exposure in Lemna gibba L.: Evaluation of growth and phytotoxic indicators, detection of ibuprofen and identification of its metabolites in plant and in the medium. Journal of Hazardous Materials, 300(1): 189–193CrossRefGoogle Scholar
  30. Qim Y, Li D, Yang H (2007). Population diversity and community structure of bacteria on biofilms in a potable water pretreating bioreactor. Chinese Journal of Applied and Environmental Biology, 13(1): 104–107Google Scholar
  31. Shen P, Chen X (2007). Microbiology experiment. In: Shen P, Chen X, eds. Physiological and Biochemical Experiment for Microorganism Identification. 4th ed. Beijing: Higher Education Press, 111–127(in Chinese)Google Scholar
  32. Tran N H, Li J, Hu J, Ong S L (2014a). Occurrence and suitability of pharmaceuticals and personal care products as molecular markers for raw wastewater contamination in surface water and groundwater. Environmental Science and Pollution Research International, 21(6): 4727–4740CrossRefGoogle Scholar
  33. Tran N H, Urase T, Ta T T (2014b). A preliminary study on the occurrence of pharmaceutically active compounds in hospital wastewater and surface water in Hanoi, Vietnam. Clean- Soil Air Water, 42(1): 267–275CrossRefGoogle Scholar
  34. Trzcinski A P, Ganda L, Kunacheva C, Zhang D Q, Lin L L, Tao G, Lee Y, Ng W J (2016). Characterization and biodegradability of sludge from a high rate A-stage contact tank and B-stage membrane bioreactor of a pilot-scale AB system treating municipal wastewaters. Water Science &Technology, 74(7): 1716–1725CrossRefGoogle Scholar
  35. Wang K, Li W, Gong X, Li X, Liu W, He C, Wang Z, Minh Q N, Chen C, Wang J (2014). Biological pretreatment of tannery wastewater using a full-scale hydrolysis acidification system. International Biodeterioration & Biodegradation, 95(Part A): 41–45CrossRefGoogle Scholar
  36. Zhang Y, Lv T, Carvalho P N, Arias C A, Chen Z, Brix H (2016). Removal of the pharmaceuticals ibuprofen and iohexol by four wetland plant species in hydroponic culture: plant uptake and microbial degradation. Environmental Science and Pollution Research International, 23(3): 2890–2898CrossRefGoogle Scholar
  37. Zhao W, Guo Y, Lu S, Yan P, Sui Q (2016). Recent advances in pharmaceuticals and personal care products in the surface water and sediments in China. Frontiers of Environmental Science & Engineering, 10(6): 2CrossRefGoogle Scholar

Copyright information

© Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Chemistry and Environmental EngineeringJiujiang UniversityJiujiangChina
  2. 2.College of Environmental Science and EngineeringDonghua UniversityShanghaiChina

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