Journal of Central South University

, Volume 18, Issue 5, pp 1408–1412 | Cite as

Heavy metals accumulation effect in rabbit body fluids after smoking

  • Kuang-biao Zhong (钟狂飚)
  • Ming Gui (桂明)Email author
  • Li-yong Zhu (朱立勇)
  • Wei Li (李维)
  • Cha-xiang Guan (管茶香)
  • Fang-qiu Guo (郭方遒)


Concentration of heavy metals in blood and urine of rabbit after inhaling three different kinds of cigarette was studied through the animal passive smoking pattern. The samples were prepared by nitric acid solution digestion and determination of seven kinds of heavy metals including Hg, Se, Sn, Pb, Cd, Ni and Cr was performed by inductively coupled plasma-atomic emission spectrometry (ICP-AES). The ICP-AES method was established with good precision and accuracy, relative standard deviation (n=6) was between 2.9% and 5.9%, and the recovery was in the range of 95.0%–104.2%. Concentration of six heavy metals increases in some extent in blood and urine after period of smoking and the increasing of heavy metals in blood and urine all shows time dependence. Significantly higher heavy metal levels are observed in the blood and urine of the cigarette inhaling rabbits in the exposed group. The concentration of six kinds of heavy metals in the blood of the rabbit increases after 16 weeks exposing to cigarette smoking. Three times of Hg, ten times of Se and trace amount of Pb, Cd, Ni and Cr are detected in the blood after 16 weeks of smoking. For urine samples, about three times of Hg, two times of Se, five times of Pb and trace amount of Cd are detected after 16 weeks of inhalation of cigarette. Comparatively, higher concentration of heavy metals are detected after inhaling of Nise cigarette.

Key words

inductively coupled plasma-atomic emission spectrometry blood urine heavy metal element 


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  1. [1]
    Geneva: World Health Organization [EB/OL]. 2008-04-04.
  2. [2]
    XI Zhu-ge, CHAO Fu-huan, SUN Yong-mei, YANG Feng-dan, ZHANG Hua-shan, LI Guan-xian, LI Yuan. Study on the mechanism of oxidative damage of DNA induced by reactive oxygen species due to metal ions [J]. Acta Scientiae Circumstantiae, 2003, 23(5): 662–667.Google Scholar
  3. [3]
    SCHNEIDER G, KRIVAN V. Multi-elemental analysis of tobacco and smoke condensate by instrumental neutron activation analysis and atomic absorption spectrometry [J]. Int J Environ Anal Chem, 1993, 53(2): 87–100.CrossRefGoogle Scholar
  4. [4]
    ZHU Zhi-guo, WANG Gui-xian, CHEN Jing-hua. Determination of potassium, calcium, manganese, chromium and cadmium in 10 kinds of cigarettes by flame atomic absorption spectrophotometer [J]. Spectroscopy and Spectral Analysis, 1999, 19(2): 210–211.Google Scholar
  5. [5]
    VERMA S, YADAV S, SINGH I. Trace metal concentration in different Indian tobacco products and related health implications [J]. Food and Chemical Toxicology, 2010, 48(8/9): 2291–2297.CrossRefGoogle Scholar
  6. [6]
    ANGELOVA V, IVANOV K, IVANOVA R. Effect of chemical forms of lead, cadmium and zinc in polluted soils on their uptake by tobacco [J]. Journal of Plant Nutrition, 2004, 27(5): 757–773.CrossRefGoogle Scholar
  7. [7]
    QIU Xiao-hui, WEI De-ren. Effect of content of metal elements in blood serum [J]. Journal of Public Health, 1994, 10(1): 20–24.Google Scholar
  8. [8]
    GUPTA J P. Determination of yttrium and rare-earth elements in rocks by graphite-furnace atomic-absorption spectrometry [J]. Talanta, 1981, 28(1): 31–36.CrossRefGoogle Scholar
  9. [9]
    HAVEL J, MORENO C, HRDLICKA A, VALIENTE M. Spectrophotometric determination of rare earth elements by flow injection analysis based on their reaction with xylenol orange and cetylpyridinium bromide [J]. Talanta, 1994, 41(8): 1251–1254.CrossRefGoogle Scholar
  10. [10]
    BRAVERMAN D S. Determination of rare earth elements by liquid chromatographic separation using inductively coupled plasma mass spectrometric detection [J]. J Anal At Spectrom, 1992, 7(1): 43–46.CrossRefGoogle Scholar
  11. [11]
    WANG Xiao-yan, LI Yu-feng, LI Bai. Fast determination of heavy metals in human blood and urine samples by ICP-MS after simple dilution [J]. Chinese Journal of Analysis Laboratory, 2010, 29(6): 41–45.Google Scholar
  12. [12]
    CROUDACE I W, MARSHALL S. Determination of rare earth elements and yttrium in nine geochemical reference samples using a novel group separation procedure involving mixed-acid elution ion-exchange chromatography [J]. Geostand Newsl, 1991, 15(1): 139–145.CrossRefGoogle Scholar
  13. [13]
    GUPTA J P. Analysis of the CCRMP Oka-2 rare-earth reference mineral of the britholite-apatite series by electrothermal atomicabsorption and inductively-coupled plasma atomicemission spectrometry [J]. Talanta, 1987, 34(12): 1043–1047.CrossRefGoogle Scholar
  14. [14]
    EID M A, BROEKAERT J A C, TSCHÖPEL P. Application of ICPAES to the determination of rare earth elements in phosphate samples [J]. Fresenius J Anal Chem, 1992, 342(1/2): 107–112.CrossRefGoogle Scholar
  15. [15]
    WALSH J N, BUCKLEY F, BARKER J. The simultaneous determination of the rare-earth elements in rocks using inductively coupled plasma source spectrometry [J]. Chem Geol, 1981, 33(1/2/3/4): 141–153.CrossRefGoogle Scholar

Copyright information

© Central South University Press and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Kuang-biao Zhong (钟狂飚)
    • 1
  • Ming Gui (桂明)
    • 1
    Email author
  • Li-yong Zhu (朱立勇)
    • 1
  • Wei Li (李维)
    • 1
  • Cha-xiang Guan (管茶香)
    • 1
  • Fang-qiu Guo (郭方遒)
    • 2
  1. 1.Urology Surgery of The Third Xiangya HospitalCentral South UniversityChangshaChina
  2. 2.School of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina

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