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Journal of Central South University

, Volume 18, Issue 5, pp 1408–1412 | Cite as

Heavy metals accumulation effect in rabbit body fluids after smoking

  • Kuang-biao Zhong (钟狂飚)
  • Ming Gui (桂明)Email author
  • Li-yong Zhu (朱立勇)
  • Wei Li (李维)
  • Cha-xiang Guan (管茶香)
  • Fang-qiu Guo (郭方遒)
Article

Abstract

Concentration of heavy metals in blood and urine of rabbit after inhaling three different kinds of cigarette was studied through the animal passive smoking pattern. The samples were prepared by nitric acid solution digestion and determination of seven kinds of heavy metals including Hg, Se, Sn, Pb, Cd, Ni and Cr was performed by inductively coupled plasma-atomic emission spectrometry (ICP-AES). The ICP-AES method was established with good precision and accuracy, relative standard deviation (n=6) was between 2.9% and 5.9%, and the recovery was in the range of 95.0%–104.2%. Concentration of six heavy metals increases in some extent in blood and urine after period of smoking and the increasing of heavy metals in blood and urine all shows time dependence. Significantly higher heavy metal levels are observed in the blood and urine of the cigarette inhaling rabbits in the exposed group. The concentration of six kinds of heavy metals in the blood of the rabbit increases after 16 weeks exposing to cigarette smoking. Three times of Hg, ten times of Se and trace amount of Pb, Cd, Ni and Cr are detected in the blood after 16 weeks of smoking. For urine samples, about three times of Hg, two times of Se, five times of Pb and trace amount of Cd are detected after 16 weeks of inhalation of cigarette. Comparatively, higher concentration of heavy metals are detected after inhaling of Nise cigarette.

Key words

inductively coupled plasma-atomic emission spectrometry blood urine heavy metal element 

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Copyright information

© Central South University Press and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Kuang-biao Zhong (钟狂飚)
    • 1
  • Ming Gui (桂明)
    • 1
    Email author
  • Li-yong Zhu (朱立勇)
    • 1
  • Wei Li (李维)
    • 1
  • Cha-xiang Guan (管茶香)
    • 1
  • Fang-qiu Guo (郭方遒)
    • 2
  1. 1.Urology Surgery of The Third Xiangya HospitalCentral South UniversityChangshaChina
  2. 2.School of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina

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