Journal of Central South University

, Volume 18, Issue 2, pp 381–385 | Cite as

Effect of L-cysteine on bioleaching of Ni-Cu sulphide by A. manzaensis

  • Zhi-guo He (贺治国)
  • Jian-cun Zhao (赵健存)
  • Wan-jie Liang (梁万洁)
  • Yue-hua Hu (胡岳华)Email author
  • Guan-zhou Qiu (邱冠周)


The effect of L-cysteine in different concentrations on the bioleaching of Ni-Cu sulfide was studied with an extremely thermophilic archaea, Acidianus manzaensis. It is found that adding certain amounts of L-cysteine to the bioleaching system of Ni-Cu sulfide largely enhances the leaching rate. X-ray diffraction (XRD) patterns show the change of bioleached solid residues and the effect of L-cysteine on the surface charges of minerals. Zeta potential and IR spectra of mineral surface show that the interaction between L-cysteine and mineral leads to the formation of metal complex, which is propitious to the bioleaching of Ni-Cu sulfide by Acidianus manzaensis.

Key words

Acidianus manzaensis L-cysteine Ni-Cu sulfide bioleaching surface charge Zeta potential 


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  1. [1]
    FU Jian-hua, QIU Guan-zhou, LIU Jian-she, HU Yue-hua, ZHANG Zai-hai. Study on bacterial leaching of oxide-sulphide copper ores [J]. Mining and Metallurgical Engineering, 2003, 23(4): 30–34.Google Scholar
  2. [2]
    HE Zhi-guo, GAO Feng-ling, ZHONG Hui, HU Yue-hua. Effects of L-cysteine on Ni-Cu sulfide and marmatite bioleaching by Acidithiobacillus caldus [J]. Bioresource Technology, 2009, 100(3): 1383–1387.CrossRefGoogle Scholar
  3. [3]
    HU Yue-hua, HE Zhi-guo, HU Wei-xin, PENG Hong, ZHONG Hui. Effect of two kinds of amino-acids on bioleaching metal sulfide [J]. Transactions of Nonferrous Metals Society of China, 2004, 14(4): 794–797.Google Scholar
  4. [4]
    LI Hong-mei, KE Jia-jun. The progress in bioleaching of nickel-bearing sulfide ores [J]. Multipurpose Utilization of Mineral Resources, 1999, 5(3): 28–33.Google Scholar
  5. [5]
    LIU Jian-she, WANG Zhao-hui, LI Bang-mei, ZHANG Yan-hua. Interaction between pyrite and cysteine [J]. Transactions of Nonferrous Metals Society of China, 2006, 16(4): 943–946.CrossRefGoogle Scholar
  6. [6]
    LIU Jing-ping, LI Jin, GE Xing. Synthesis and free radical inhibition rate of copper(II), iron(II), manganese(II) complexes with cysteine [J]. Chemical World, 2004(5): 235–238. (in Chinese)Google Scholar
  7. [7]
    LIU Zhong, YANG Wen-bo, BAI Gang, TIAN Wang, JIN Yon-jie. Microbial enzyme conversion of L-cysteine and L-cystine [J]. Microbiology, 2003, 30(6): 16–21. (In Chinese)Google Scholar
  8. [8]
    MIN Xiao-bo, CHAI Li-yuan, CHEN Wei-liang, ZHANG Chuan-fu, HUANG Bai-yun, KUANG Zhong. Study on bioleaching of refractory gold ore (I)—Mechanism on bioleaching of pyrite by Thiobacillus ferroxidans [J]. Transactions of Nonferrous Metals Society of China, 2001, 11(5): 784–789.Google Scholar
  9. [9]
    YOSHIDA N, NAKASATO M, OHMURA N, ANDO A, SAIKI H, ISHII M, IGARASHI Y. Acidianus manzaensis sp. nov., a novel thermoacidophilic archaeon growing autotrophically by the oxidation of H2 with the reduction of Fe3+ [J]. Current Microbiology, 2006, 53(5): 406–411.CrossRefGoogle Scholar
  10. [10]
    PARKER A, KLAUBER C, KOUGIANOS A, WATLING H R, VAN BRONSWIJK W. An X-ray photoelectron spectroscopy study of the mechanism of oxidative dissolution of chalcopyrite [J]. Hydrometallurgy, 2003, 71(1/2): 265–276.CrossRefGoogle Scholar
  11. [11]
    QIU Guan-zhou, LI Quan, QIN Wen-qing. Bacterial leaching complex sulfide minerals of lead, antimony, zinc and iron [J]. Mining and Metallurgical Engineering, 2005, 25(3): 30–33.Google Scholar
  12. [12]
    QIU Guan-zhou, LIU Jian-she, HU Yue-hua. Electrochemical behavior of chalcopyrite in presence of Thiobacillus ferroxidans [J]. Transactions of Nonferrous Metals Society of China, 2000, 10(S1): 23–25.Google Scholar
  13. [13]
    ROJAS-CHAPANA J A, BARTELS C C, POHLMANN I, TRIBUTSCH H. Co-operative leaching and chemotaxis of thiobacilli studied with spherical sulphumulphide substrates [J]. Process Biochem, 1998, 33(3): 239–248.CrossRefGoogle Scholar
  14. [14]
    ROJAS-CHAPANA J A, GIERSIG M, TRIBUTSCH H. The path of sulphur during the bio-oxidation of pyrite by Thiobacillus ferrooxidans [J]. Fuel, 1996, 75(8): 923–930.CrossRefGoogle Scholar
  15. [15]
    ROJAS-CHAPANA J A, TRIBUTSCH H. Biochemistry of sulfur extraction in bio-corrosion of pyrite by Thiobacillus ferrooxidans [J]. Hydrometallurgy, 2001, 59(2/3): 291–300.CrossRefGoogle Scholar
  16. [16]
    ROJAS-CHAPANA J A, TRIBUTSCH H. Bio-leaching of pyrite accelerated by cysteine [J]. Process Biochemistry, 2000, 35(8): 815–824.CrossRefGoogle Scholar
  17. [17]
    SANO Y, TAKEDA S. Cytotoxicity and dissolution of metallic biomaterials using dynamic extraction (in vitro) [J]. Shika Igaku, 1992, 55(2): 125–140.Google Scholar
  18. [18]
    SUZUKI I. Microbial leaching of metals from sulphide minerals [J]. Biotechnology Advances, 2001, 19(2): 119–132.CrossRefGoogle Scholar
  19. [19]
    TRIBUTSCH H, BENNET J C. Semiconductor-electrochemical aspects of bacterial leaching: I. Oxidation of metal sulphides with large energy gaps [J]. J Chem Techno1 Biotechnol, 1981, 31(1): 565–511.CrossRefGoogle Scholar
  20. [20]
    TRIBUTSCH H, ROJAS-CHAPANA J A. Metal sulfide semiconductor with the reduction of Fe3+ [J]. Current Microbiology, 2006, 53(3): 406–411.Google Scholar
  21. [21]
    YUE Song. Preparations and structure characterization of the crystals of ferrous cysteine [J]. Chemical Research and Application, 2000, 12(4): 387–390. (in Chinese)Google Scholar

Copyright information

© Central South University Press and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Zhi-guo He (贺治国)
    • 1
    • 2
  • Jian-cun Zhao (赵健存)
    • 1
    • 2
  • Wan-jie Liang (梁万洁)
    • 1
    • 2
  • Yue-hua Hu (胡岳华)
    • 1
    • 2
    Email author
  • Guan-zhou Qiu (邱冠周)
    • 1
    • 2
  1. 1.School of Minerals Processing and BioengineeringCentral South UniversityChangshaChina
  2. 2.Key Laboratory of Biometallurgy of Ministry of EducationCentral South UniversityChangshaChina

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